Plant immune memory in systemic tissue does not involve changes in rapid calcium signaling
Upon pathogen recognition, a transient rise in cytoplasmic calcium levels is one of the earliest events in plants and a prerequisite for defense initiation and signal propagation from a local site to systemic plant tissues. However, it is unclear if calcium signaling differs in the context of primin...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
14 December 2021
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| In: |
Frontiers in plant science
Year: 2021, Jahrgang: 12, Pages: 1-14 |
| ISSN: | 1664-462X |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/article/10.3389/fpls.2021.798230
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| Verfasserangaben: | Bernadette Eichstädt, Sarah Lederer, Fabian Trempel, Xiyuan Jiang, Tiziana Guerra, Rainer Waadt, Justin Lee, Anja Liese, Tina Romeis |
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| 245 | 1 | 0 | |a Plant immune memory in systemic tissue does not involve changes in rapid calcium signaling |c Bernadette Eichstädt, Sarah Lederer, Fabian Trempel, Xiyuan Jiang, Tiziana Guerra, Rainer Waadt, Justin Lee, Anja Liese, Tina Romeis |
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| 520 | |a Upon pathogen recognition, a transient rise in cytoplasmic calcium levels is one of the earliest events in plants and a prerequisite for defense initiation and signal propagation from a local site to systemic plant tissues. However, it is unclear if calcium signaling differs in the context of priming: Do plants exposed to a first pathogen stimulus and have consequently established systemic acquired resistance (SAR) display altered calcium responses to a second pathogen stimulus? Several calcium indicator systems including aequorin, YC3.6 or R-GECO1 have been used to document local calcium responses to the bacterial flg22 peptide but systemic calcium imaging within a single plant remains a technical challenge. Here, we report on an experimental approach to monitor flg22-induced calcium responses in systemic leaves of primed plants. The calcium-dependent protein kinase CPK5 is a key calcium sensor and regulator of the NADPH oxidase RBOHD and plays a role in the systemic calcium-ROS signal propagation. We therefore compared flg22-induced cytoplasmic calcium changes in Arabidopsis wild-type, cpk5 mutant and CPK5-overexpressing plants (exhibiting constitutive priming) by introgressing the calcium indicator R-GECO1-mTurquoise that allows internal normalization through mTurquoise fluorescence. Aequorin-based analyses were included for comparison. Based on the R-GECO1-mTurquoise data, CPK5-OE appears to reinforce an “oscillatory-like” Ca2+ signature in flg22-treated local tissues. However, no change was observed in the flg22-induced calcium response in the systemic tissues of plants that had been pre-challenged by a priming stimulus - neither in wild-type nor in cpk5 or CPK5-OE-lines. These data indicate that the mechanistic manifestation of a plant immune memory in distal plant parts required for enhanced pathogen resistance does not include changes in rapid calcium signaling upstream of CPK5 but rather relies on downstream defense responses. | ||
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| 700 | 1 | |a Trempel, Fabian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jiang, Xiyuan |d 1986- |e VerfasserIn |0 (DE-588)1200445414 |0 (DE-627)1683552083 |4 aut | |
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