A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage
Hydroperoxide detoxification in African trypanosomes is achieved by 2-Cys-peroxiredoxin (TXNPx)- and non-selenium glutathione peroxidase (Px)-type enzymes which both obtain their reducing equivalents from the unique trypanothione/tryparedoxin system. Previous RNA interference approaches revealed tha...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2011
|
| In: |
Free radical biology and medicine
Year: 2011, Jahrgang: 51, Heft: 4, Pages: 856-868 |
| ISSN: | 1873-4596 |
| DOI: | 10.1016/j.freeradbiomed.2011.05.014 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.freeradbiomed.2011.05.014 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0891584911003169 
|
| Verfasserangaben: | Michael Diechtierow, R. Luise Krauth-Siegel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1801311110 | ||
| 003 | DE-627 | ||
| 005 | 20230427140309.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220510s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.freeradbiomed.2011.05.014 |2 doi | |
| 035 | |a (DE-627)1801311110 | ||
| 035 | |a (DE-599)KXP1801311110 | ||
| 035 | |a (OCoLC)1341459489 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Diechtierow, Michael |e VerfasserIn |0 (DE-588)1017814287 |0 (DE-627)690431988 |0 (DE-576)354370448 |4 aut | |
| 245 | 1 | 2 | |a A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage |c Michael Diechtierow, R. Luise Krauth-Siegel |
| 264 | 1 | |c 2011 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 10.05.2022 | ||
| 520 | |a Hydroperoxide detoxification in African trypanosomes is achieved by 2-Cys-peroxiredoxin (TXNPx)- and non-selenium glutathione peroxidase (Px)-type enzymes which both obtain their reducing equivalents from the unique trypanothione/tryparedoxin system. Previous RNA interference approaches revealed that the cytosolic TXNPx and the Px-type enzymes are essential for Trypanosoma brucei. Because of partially overlapping in vitro substrate specificities and subcellular localisation the physiological function of the individual enzymes was not yet clear. As shown here, TXNPx and Px are expressed at comparable levels and in their active reduced state. Px-overexpressing parasites were less sensitive toward linoleic acid hydroperoxide but not hydrogen peroxide. Kinetic studies confirmed that Px—but not TXNPx—reduces lipophilic hydroperoxides including phospholipids with high efficiency. Most interestingly, the severe proliferation defect of Px-depleted bloodstream cells could be rescued by Trolox, but not by hydrophilic antioxidants, in the medium. This allowed us to knock-out the three Px genes individually and thus to distinguish their in vivo role. Deletion of the cytosolic Px I and II resulted in extremely fast membrane peroxidation followed by cell lysis. Cells lacking specifically the mitochondrial Px III showed a transient growth retardation and cardiolipin peroxidation but adapted within 24h to normal proliferation. | ||
| 650 | 4 | |a Gene knock-out | |
| 650 | 4 | |a Glutathione peroxidase | |
| 650 | 4 | |a Lipid peroxidation | |
| 650 | 4 | |a Membrane damage | |
| 650 | 4 | |a TBAR | |
| 650 | 4 | |a Trolox | |
| 650 | 4 | |a Trypanothione | |
| 650 | 4 | |a Tryparedoxin peroxidase | |
| 700 | 1 | |a Krauth-Siegel, Renate |e VerfasserIn |0 (DE-588)1050022335 |0 (DE-627)783217390 |0 (DE-576)404300200 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Free radical biology and medicine |d New York, NY [u.a.] : Elsevier, 1987 |g 51(2011), 4, Seite 856-868 |h Online-Ressource |w (DE-627)300898568 |w (DE-600)1483653-1 |w (DE-576)098330233 |x 1873-4596 |7 nnas |a A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage |
| 773 | 1 | 8 | |g volume:51 |g year:2011 |g number:4 |g pages:856-868 |g extent:13 |a A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.freeradbiomed.2011.05.014 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0891584911003169 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220510 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1050022335 |a Krauth-Siegel, Renate |m 1050022335:Krauth-Siegel, Renate |d 700000 |d 711000 |e 700000PK1050022335 |e 711000PK1050022335 |k 0/700000/ |k 1/700000/711000/ |p 2 |y j | ||
| 998 | |g 1017814287 |a Diechtierow, Michael |m 1017814287:Diechtierow, Michael |d 140000 |e 140000PD1017814287 |k 0/140000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1801311110 |e 413164021X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1801311110"],"doi":["10.1016/j.freeradbiomed.2011.05.014"]},"origin":[{"dateIssuedDisp":"2011","dateIssuedKey":"2011"}],"name":{"displayForm":["Michael Diechtierow, R. Luise Krauth-Siegel"]},"relHost":[{"language":["eng"],"recId":"300898568","note":["Ungezählte Beil.: Supplement"],"disp":"A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damageFree radical biology and medicine","type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"extent":"13","volume":"51","text":"51(2011), 4, Seite 856-868","pages":"856-868","issue":"4","year":"2011"},"titleAlt":[{"title":"Free radical biology & medicine"}],"pubHistory":["3.1987 -"],"title":[{"title":"Free radical biology and medicine","subtitle":"the official journal of the Oxygen Society, a constituent member of the International Society for Free Radical Research","title_sort":"Free radical biology and medicine"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["1483653-1"],"eki":["300898568"],"issn":["1873-4596"]},"origin":[{"dateIssuedDisp":"1987-","publisher":"Elsevier","dateIssuedKey":"1987","publisherPlace":"New York, NY [u.a.]"}]}],"physDesc":[{"extent":"13 S."}],"title":[{"title_sort":"tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage","title":"A tryparedoxin-dependent peroxidase protects African trypanosomes from membrane damage"}],"person":[{"family":"Diechtierow","given":"Michael","roleDisplay":"VerfasserIn","display":"Diechtierow, Michael","role":"aut"},{"display":"Krauth-Siegel, Renate","roleDisplay":"VerfasserIn","role":"aut","family":"Krauth-Siegel","given":"Renate"}],"recId":"1801311110","language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 10.05.2022"]} | ||
| SRT | |a DIECHTIEROTRYPAREDOX2011 | ||