Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking
The viral polyprotein Gag plays a central role for HIV-1 assembly, release and maturation. Proteolytic processing of Gag by the viral protease is essential for the structural rearrangements that mark the transition from immature to mature, infectious viruses. The timing and kinetics of Gag processin...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 February 2022
|
| In: |
Viruses
Year: 2022, Jahrgang: 14, Heft: 2, Pages: 1-13 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v14020340 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/v14020340 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1999-4915/14/2/340 
|
| Verfasserangaben: | Chen Qian, Annica Flemming, Barbara Müller and Don C. Lamb |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1801818460 | ||
| 003 | DE-627 | ||
| 005 | 20230426073531.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220514s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/v14020340 |2 doi | |
| 035 | |a (DE-627)1801818460 | ||
| 035 | |a (DE-599)KXP1801818460 | ||
| 035 | |a (OCoLC)1341459868 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Qian, Chen |e VerfasserIn |0 (DE-588)1257539736 |0 (DE-627)1801818398 |4 aut | |
| 245 | 1 | 0 | |a Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking |c Chen Qian, Annica Flemming, Barbara Müller and Don C. Lamb |
| 264 | 1 | |c 8 February 2022 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.05.2022 | ||
| 520 | |a The viral polyprotein Gag plays a central role for HIV-1 assembly, release and maturation. Proteolytic processing of Gag by the viral protease is essential for the structural rearrangements that mark the transition from immature to mature, infectious viruses. The timing and kinetics of Gag processing are not fully understood. Here, fluorescence lifetime imaging microscopy and single virus tracking are used to follow Gag processing in nascent HIV-1 particles in situ. Using a Gag polyprotein labelled internally with eCFP, we show that proteolytic release of the fluorophore from Gag is accompanied by an increase in its fluorescence lifetime. By tracking nascent virus particles in situ and analyzing the intensity and fluorescence lifetime of individual traces, we detect proteolytic cleavage of eCFP from Gag in a subset (6.5%) of viral particles. This suggests that for the majority of VLPs, Gag processing occurs with a delay after particle assembly. | ||
| 650 | 4 | |a fluorescence lifetime | |
| 650 | 4 | |a gag processing | |
| 650 | 4 | |a HIV | |
| 650 | 4 | |a maturation | |
| 650 | 4 | |a single virus tracking | |
| 700 | 1 | |a Flemming, Annica |e VerfasserIn |0 (DE-588)1187788791 |0 (DE-627)1666708658 |4 aut | |
| 700 | 1 | |a Müller, Barbara |d 1961- |e VerfasserIn |0 (DE-588)1028058667 |0 (DE-627)730238385 |0 (DE-576)375540024 |4 aut | |
| 700 | 1 | |a Lamb, Don C. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Viruses |d Basel : MDPI, 2009 |g 14(2022), 2 vom: Feb., Artikel-ID 340, Seite 1-13 |h Online-Ressource |w (DE-627)609775871 |w (DE-600)2516098-9 |w (DE-576)311326080 |x 1999-4915 |7 nnas |a Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking |
| 773 | 1 | 8 | |g volume:14 |g year:2022 |g number:2 |g month:02 |g elocationid:340 |g pages:1-13 |g extent:13 |a Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking |
| 856 | 4 | 0 | |u https://doi.org/10.3390/v14020340 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/1999-4915/14/2/340 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220514 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 1028058667 |a Müller, Barbara |m 1028058667:Müller, Barbara |d 910000 |d 911700 |d 50000 |e 910000PM1028058667 |e 911700PM1028058667 |e 50000PM1028058667 |k 0/910000/ |k 1/910000/911700/ |k 0/50000/ |p 3 | ||
| 998 | |g 1187788791 |a Flemming, Annica |m 1187788791:Flemming, Annica |p 2 | ||
| 999 | |a KXP-PPN1801818460 |e 4134228166 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 14.05.2022"],"origin":[{"dateIssuedDisp":"8 February 2022","dateIssuedKey":"2022"}],"title":[{"title":"Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking","title_sort":"Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus tracking"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1801818460","relHost":[{"language":["eng"],"part":{"extent":"13","volume":"14","pages":"1-13","year":"2022","text":"14(2022), 2 vom: Feb., Artikel-ID 340, Seite 1-13","issue":"2"},"recId":"609775871","name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["609775871"],"zdb":["2516098-9"],"issn":["1999-4915"]},"pubHistory":["1.2009 -"],"origin":[{"dateIssuedDisp":"2009-","dateIssuedKey":"2009","publisher":"MDPI","publisherPlace":"Basel"}],"title":[{"title_sort":"Viruses","title":"Viruses"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Dynamics of HIV-1 gag processing as revealed by fluorescence lifetime imaging microscopy and single virus trackingViruses"}],"name":{"displayForm":["Chen Qian, Annica Flemming, Barbara Müller and Don C. Lamb"]},"physDesc":[{"extent":"13 S."}],"id":{"eki":["1801818460"],"doi":["10.3390/v14020340"]},"person":[{"family":"Qian","display":"Qian, Chen","given":"Chen","role":"aut"},{"family":"Flemming","display":"Flemming, Annica","given":"Annica","role":"aut"},{"given":"Barbara","role":"aut","family":"Müller","display":"Müller, Barbara"},{"family":"Lamb","display":"Lamb, Don C.","given":"Don C.","role":"aut"}],"language":["eng"]} | ||
| SRT | |a QIANCHENFLDYNAMICSOF8202 | ||