Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord
The spinal cord is the main pathway connecting brain and peripheral nervous system. Its functionality relies on the orchestrated activity of both neurons and glial cells. To date, most advancement in understanding the spinal cord inner mechanisms has been made either by in vivo exposure of its dorsa...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
30 March 2022
|
| In: |
Frontiers in molecular neuroscience
Year: 2022, Jahrgang: 15, Pages: 1-16 |
| ISSN: | 1662-5099 |
| DOI: | 10.3389/fnmol.2022.840948 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3389/fnmol.2022.840948 Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/article/10.3389/fnmol.2022.840948 
|
| Verfasserangaben: | Phillip Rieder, Davide Gobbo, Gebhard Stopper, Anna Welle, Elisa Damo, Frank Kirchhoff and Anja Scheller |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1802595422 | ||
| 003 | DE-627 | ||
| 005 | 20250107202637.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220519s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fnmol.2022.840948 |2 doi | |
| 035 | |a (DE-627)1802595422 | ||
| 035 | |a (DE-599)KXP1802595422 | ||
| 035 | |a (OCoLC)1341460298 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Rieder, Phillip |d 1990- |e VerfasserIn |0 (DE-588)1257912828 |0 (DE-627)1802596771 |4 aut | |
| 245 | 1 | 0 | |a Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord |c Phillip Rieder, Davide Gobbo, Gebhard Stopper, Anna Welle, Elisa Damo, Frank Kirchhoff and Anja Scheller |
| 246 | 3 | 0 | |a two plus |
| 246 | 3 | 3 | |a Astrocytes and microglia exhibit cell-specific Ca 2+ signaling dynamics in the murine spinal cord |
| 264 | 1 | |c 30 March 2022 | |
| 300 | |a 16 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Text ist "2+" hochgestellt | ||
| 500 | |a Gesehen am 19.05.2022 | ||
| 520 | |a The spinal cord is the main pathway connecting brain and peripheral nervous system. Its functionality relies on the orchestrated activity of both neurons and glial cells. To date, most advancement in understanding the spinal cord inner mechanisms has been made either by in vivo exposure of its dorsal surface through laminectomy or by acute ex vivo slice preparation, likely affecting spinal cord physiology in virtue of the necessary extensive manipulation of the spinal cord tissue. This is especially true of cells immediately responding to alterations of the surrounding environment, such as microglia and astrocytes, reacting within seconds or minutes and for up to several days after the original insult. Ca2+ signaling is considered one of the most immediate, versatile, and yet elusive cellular responses of glia. Here, we induced the cell-specific expression of the genetically encoded Ca2+ indicator GCaMP3 to evaluate spontaneous intracellular Ca2+ signaling in astrocytes and microglia. Ca2+ signals were then characterized in acute ex vivo (both gray and white matter) as well as in chronic in vivo (white matter) preparations using MSparkles, a MATLAB-based software for automatic detection and analysis of fluorescence events. As a result, we were able to segregate distinct astroglial and microglial Ca2+ signaling patterns along with method-specific Ca2+ signaling alterations, which must be taken into consideration in the reliable evaluation of any result obtained in physiological as well as pathological conditions. Our study revealed a high degree of Ca2+ signaling diversity in glial cells of the murine spinal cord, thus adding to the current knowledge of the astonishing glial heterogeneity and cell-specific Ca2+ dynamics in non-neuronal networks. | ||
| 700 | 1 | |a Gobbo, Davide |e VerfasserIn |4 aut | |
| 700 | 1 | |a Stopper, Gebhard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Welle, Anna |e VerfasserIn |4 aut | |
| 700 | 1 | |a Damo, Elisa |d 1995- |e VerfasserIn |0 (DE-588)1257913336 |0 (DE-627)1802597883 |4 aut | |
| 700 | 1 | |8 1\p |a Kirchhoff, Frank |d 1960- |e VerfasserIn |0 (DE-588)132256857X |0 (DE-627)1882474473 |4 aut | |
| 700 | 1 | |a Scheller, Anja |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in molecular neuroscience |d Lausanne : Frontiers Research Foundation, 2008 |g 15(2022), Artikel-ID 840948, Seite 1-16 |h Online-Ressource |w (DE-627)579826449 |w (DE-600)2452967-9 |w (DE-576)286502267 |x 1662-5099 |7 nnas |a Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord |
| 773 | 1 | 8 | |g volume:15 |g year:2022 |g elocationid:840948 |g pages:1-16 |g extent:16 |a Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fnmol.2022.840948 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/article/10.3389/fnmol.2022.840948 |x Verlag |z lizenzpflichtig |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20220519 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 1257913336 |a Damo, Elisa |m 1257913336:Damo, Elisa |d 50000 |d 54600 |e 50000PD1257913336 |e 54600PD1257913336 |k 0/50000/ |k 1/50000/54600/ |p 5 | ||
| 999 | |a KXP-PPN1802595422 |e 4136178824 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2022","dateIssuedDisp":"30 March 2022"}],"id":{"doi":["10.3389/fnmol.2022.840948"],"eki":["1802595422"]},"name":{"displayForm":["Phillip Rieder, Davide Gobbo, Gebhard Stopper, Anna Welle, Elisa Damo, Frank Kirchhoff and Anja Scheller"]},"physDesc":[{"extent":"16 S."}],"relHost":[{"part":{"extent":"16","volume":"15","text":"15(2022), Artikel-ID 840948, Seite 1-16","pages":"1-16","year":"2022"},"pubHistory":["2008 -"],"recId":"579826449","language":["eng"],"disp":"Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cordFrontiers in molecular neuroscience","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 04.06.20"],"title":[{"title":"Frontiers in molecular neuroscience","title_sort":"Frontiers in molecular neuroscience"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["579826449"],"zdb":["2452967-9"],"issn":["1662-5099"]},"origin":[{"publisherPlace":"Lausanne","publisher":"Frontiers Research Foundation","dateIssuedKey":"2008","dateIssuedDisp":"2008-"}],"name":{"displayForm":["Frontiers Research Foundation"]}}],"title":[{"title_sort":"Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord","title":"Astrocytes and microglia exhibit cell-specific Ca2+ signaling dynamics in the murine spinal cord"}],"person":[{"display":"Rieder, Phillip","roleDisplay":"VerfasserIn","role":"aut","family":"Rieder","given":"Phillip"},{"roleDisplay":"VerfasserIn","display":"Gobbo, Davide","role":"aut","family":"Gobbo","given":"Davide"},{"given":"Gebhard","family":"Stopper","role":"aut","roleDisplay":"VerfasserIn","display":"Stopper, Gebhard"},{"given":"Anna","family":"Welle","role":"aut","roleDisplay":"VerfasserIn","display":"Welle, Anna"},{"role":"aut","display":"Damo, Elisa","roleDisplay":"VerfasserIn","given":"Elisa","family":"Damo"},{"roleDisplay":"VerfasserIn","display":"Kirchhoff, Frank","role":"aut","family":"Kirchhoff","given":"Frank"},{"role":"aut","display":"Scheller, Anja","roleDisplay":"VerfasserIn","given":"Anja","family":"Scheller"}],"titleAlt":[{"title":"Astrocytes and microglia exhibit cell-specific Ca 2+ signaling dynamics in the murine spinal cord"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Im Text ist \"2+\" hochgestellt","Gesehen am 19.05.2022"],"language":["eng"],"recId":"1802595422"} | ||
| SRT | |a RIEDERPHILASTROCYTES3020 | ||