Cover Image

Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay

We have developed a highly sensitive, universal assay that allows detection as well as identification of all known retroviral reverse transcriptase (RT)-related nucleic acids in a biological sample by a single two-step experiment. The assay combines polymerase chain reaction (PCR) and reverse dot-bl...

Full description

Saved in:
Bibliographic Details
Main Authors: Seifarth, Wolfgang (Author) , Krause, Ulf (Author) , Hohenadl, Christine (Author) , Baust, Corinna (Author) , Hehlmann, Rüdiger (Author) , Leib-Mösch, Christine (Author)
Format: Article (Journal)
Language:English
Published: 2000
In: Aids research and human retroviruses
Year: 2000, Volume: 16, Issue: 8, Pages: 721-729
ISSN:1931-8405
DOI:10.1089/088922200308729
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1089/088922200308729
Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/088922200308729
Get full text
Author Notes:Wolfgang Seifarth, Ulf Krause, Christine Hohenadl, Corinna Baust, Rüdiger Hehlmann, and Christine Leib-Mösch

MARC

LEADER 00000caa a2200000 c 4500
001 1802743545
003 DE-627
005 20220820185246.0
007 cr uuu---uuuuu
008 220519s2000 xx |||||o 00| ||eng c
024 7 |a 10.1089/088922200308729  |2 doi 
035 |a (DE-627)1802743545 
035 |a (DE-599)KXP1802743545 
035 |a (OCoLC)1341460041 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Seifarth, Wolfgang  |d 1960-  |e VerfasserIn  |0 (DE-588)1042491887  |0 (DE-627)769066453  |0 (DE-576)393988899  |4 aut 
245 1 0 |a Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay  |c Wolfgang Seifarth, Ulf Krause, Christine Hohenadl, Corinna Baust, Rüdiger Hehlmann, and Christine Leib-Mösch 
264 1 |c 2000 
300 |a 9 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Elektronische Reproduktion der Druckausgabe 
500 |a Gesehen am 19.05.2022 
520 |a We have developed a highly sensitive, universal assay that allows detection as well as identification of all known retroviral reverse transcriptase (RT)-related nucleic acids in a biological sample by a single two-step experiment. The assay combines polymerase chain reaction (PCR) and reverse dot-blot hybridization (RDBH), using an array of immobilized synthetic retrovirus-specific oligonucleotides and two sets of mixed oligo primers (MOPs). These primers were derived from highly conserved motifs found in all known reverse transcriptase genes. The PCR/RDBH assay was used for qualitative analyses of human endogenous retrovirus (HERV) transcription in peripheral blood mononuclear cells (PBMCs) and in particles released by the human mammary carcinoma-derived cell line T47D. Sensitivity was further demonstrated by detection of down to 10 copies of pig endogenous retrovirus (PERV) DNA in human cDNA samples. Therefore, this assay is particularly useful for the identification of retroviral sequences in xenografts as well as in recipients of xenografted tissues and organs. Moreover, it is a valuable tool to detect retroviral transcripts and particles in cell cultures used for production of therapeutic polypeptides. The assay is further suitable for monitoring vector preparation used in human gene therapy to exclude transfer of copackaged endogenous retroviruses into target cells. 
700 1 |a Krause, Ulf  |d 1973-  |e VerfasserIn  |0 (DE-588)130051985  |0 (DE-627)488792991  |0 (DE-576)297970569  |4 aut 
700 1 |a Hohenadl, Christine  |e VerfasserIn  |4 aut 
700 1 |a Baust, Corinna  |e VerfasserIn  |0 (DE-588)1089079265  |0 (DE-627)850981751  |0 (DE-576)175130981  |4 aut 
700 1 |a Hehlmann, Rüdiger  |d 1941-  |e VerfasserIn  |0 (DE-588)1037003489  |0 (DE-627)751737879  |0 (DE-576)390939463  |4 aut 
700 1 |a Leib-Mösch, Christine  |d 1950-  |e VerfasserIn  |0 (DE-588)1050970357  |0 (DE-627)785784403  |0 (DE-576)405425457  |4 aut 
773 0 8 |i Enthalten in  |t Aids research and human retroviruses  |d Larchmont, NY : Liebert, 1987  |g 16(2000), 8, Seite 721-729  |h Online-Ressource  |w (DE-627)269759085  |w (DE-600)1475767-9  |w (DE-576)095925546  |x 1931-8405  |7 nnas  |a Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay 
773 1 8 |g volume:16  |g year:2000  |g number:8  |g pages:721-729  |g extent:9  |a Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay 
856 4 0 |u https://doi.org/10.1089/088922200308729  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.liebertpub.com/doi/10.1089/088922200308729  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20220519 
993 |a Article 
994 |a 2000 
998 |g 1050970357  |a Leib-Mösch, Christine  |m 1050970357:Leib-Mösch, Christine  |d 60000  |d 61200  |e 60000PL1050970357  |e 61200PL1050970357  |k 0/60000/  |k 1/60000/61200/  |p 6  |y j 
998 |g 1037003489  |a Hehlmann, Rüdiger  |m 1037003489:Hehlmann, Rüdiger  |d 60000  |d 61200  |e 60000PH1037003489  |e 61200PH1037003489  |k 0/60000/  |k 1/60000/61200/  |p 5 
998 |g 1089079265  |a Baust, Corinna  |m 1089079265:Baust, Corinna  |d 60000  |d 61200  |e 60000PB1089079265  |e 61200PB1089079265  |k 0/60000/  |k 1/60000/61200/  |p 4 
998 |g 130051985  |a Krause, Ulf  |m 130051985:Krause, Ulf  |d 60000  |d 61200  |e 60000PK130051985  |e 61200PK130051985  |k 0/60000/  |k 1/60000/61200/  |p 2 
998 |g 1042491887  |a Seifarth, Wolfgang  |m 1042491887:Seifarth, Wolfgang  |d 60000  |d 61200  |e 60000PS1042491887  |e 61200PS1042491887  |k 0/60000/  |k 1/60000/61200/  |p 1  |x j 
999 |a KXP-PPN1802743545  |e 4136268904 
BIB |a Y 
SER |a journal 
JSO |a {"note":["Elektronische Reproduktion der Druckausgabe","Gesehen am 19.05.2022"],"id":{"doi":["10.1089/088922200308729"],"eki":["1802743545"]},"title":[{"title_sort":"Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay","title":"Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assay"}],"recId":"1802743545","language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2000","dateIssuedDisp":"2000"}],"relHost":[{"recId":"269759085","pubHistory":["3.1987 -"],"note":["Gesehen am 30.10.2018"],"disp":"Rapid identification of all known retroviral reverse transcriptase sequences with a novel versatile detection assayAids research and human retroviruses","id":{"issn":["1931-8405"],"zdb":["1475767-9"],"eki":["269759085"]},"title":[{"title_sort":"Aids research and human retroviruses","title":"Aids research and human retroviruses"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"dateIssuedDisp":"1987-","dateIssuedKey":"1987","publisherPlace":"Larchmont, NY","publisher":"Liebert"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"part":{"pages":"721-729","year":"2000","extent":"9","volume":"16","issue":"8","text":"16(2000), 8, Seite 721-729"}}],"physDesc":[{"extent":"9 S."}],"person":[{"given":"Wolfgang","role":"aut","display":"Seifarth, Wolfgang","roleDisplay":"VerfasserIn","family":"Seifarth"},{"display":"Krause, Ulf","role":"aut","given":"Ulf","roleDisplay":"VerfasserIn","family":"Krause"},{"display":"Hohenadl, Christine","role":"aut","given":"Christine","roleDisplay":"VerfasserIn","family":"Hohenadl"},{"roleDisplay":"VerfasserIn","family":"Baust","role":"aut","display":"Baust, Corinna","given":"Corinna"},{"roleDisplay":"VerfasserIn","family":"Hehlmann","role":"aut","display":"Hehlmann, Rüdiger","given":"Rüdiger"},{"roleDisplay":"VerfasserIn","family":"Leib-Mösch","role":"aut","display":"Leib-Mösch, Christine","given":"Christine"}],"name":{"displayForm":["Wolfgang Seifarth, Ulf Krause, Christine Hohenadl, Corinna Baust, Rüdiger Hehlmann, and Christine Leib-Mösch"]}} 
SRT |a SEIFARTHWORAPIDIDENT2000 

Similar Items