Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis
Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular cargos. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains controversial when and how it is remodeled into a spherical vesicle. - Here, we use 3D superresolution microscopy to d...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) Chapter/Article |
| Language: | English |
| Published: |
October 13, 2021
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| In: |
bioRxiv beta
Year: 2021, Pages: 1-29 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://www.biorxiv.org/content/10.1101/2021.10.12.463947v1
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| Author Notes: | Markus Mund, Aline Tschanz, Yu-Le Wu, Felix Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, Jonas Ries |
MARC
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| 520 | |a Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular cargos. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains controversial when and how it is remodeled into a spherical vesicle. - Here, we use 3D superresolution microscopy to determine the precise geometry of the clathrin coat at endocytic sites. Through pseudo-temporal sorting, we determine the average trajectory of clathrin remodeling during endocytosis and find that clathrin coats assemble first on flat membranes to ≈50% of the coat area, before they become rapidly and continuously bent. We introduce a mathematical model that assumes a positive feedback for curvature generation of the clathrin coat. The cooperative curvature model agrees excellently with experimental data in three cell lines, and likely describes a general pathway for clathrin coat remodeling during endocytosis. | ||
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