Buchumschlag

Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA)

A fluorescence-linked immunofiltration assay (FLIFA) was developed for the determination of heparin-induced IgG in heparin-induced thrombocytopenia (HIT) type II patients. Protein A was immobilized on a nitrocellulose membrane to bind heparin-induced IgG of HIT type II patients. Fluorescein-5-isothi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Wang, Lianchun (VerfasserIn) , Huhle, Günter (VerfasserIn) , Malsch, Reinhard (VerfasserIn) , Hoffmann, Ursula (VerfasserIn) , Song, Xuehong (VerfasserIn) , Harenberg, Job (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1999
In: Journal of immunological methods
Year: 1999, Jahrgang: 222, Heft: 1, Pages: 93-99
ISSN:1872-7905
DOI:10.1016/S0022-1759(98)00186-0
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0022-1759(98)00186-0
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022175998001860
Volltext
Verfasserangaben:Lianchun Wang, Guenter Huhle, Reinhard Malsch, Ursula Hoffmann, Xuehong Song, Job Harenberg

MARC

LEADER 00000caa a2200000 c 4500
001 1804249459
003 DE-627
005 20220820190256.0
007 cr uuu---uuuuu
008 220524s1999 xx |||||o 00| ||eng c
024 7 |a 10.1016/S0022-1759(98)00186-0  |2 doi 
035 |a (DE-627)1804249459 
035 |a (DE-599)KXP1804249459 
035 |a (OCoLC)1341460445 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Wang, Lianchun  |d 1968-  |e VerfasserIn  |0 (DE-588)120909707  |0 (DE-627)705082946  |0 (DE-576)292442653  |4 aut 
245 1 0 |a Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA)  |c Lianchun Wang, Guenter Huhle, Reinhard Malsch, Ursula Hoffmann, Xuehong Song, Job Harenberg 
264 1 |c 1999 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Available online 22 December 1998 
500 |a Gesehen am 24.05.2022 
520 |a A fluorescence-linked immunofiltration assay (FLIFA) was developed for the determination of heparin-induced IgG in heparin-induced thrombocytopenia (HIT) type II patients. Protein A was immobilized on a nitrocellulose membrane to bind heparin-induced IgG of HIT type II patients. Fluorescein-5-isothiocynate (FITC)-heparin was added to platelet factor 4 present in normal serum to form the neo-antigen which was captured by heparin-induced IgG. The heparin-induced IgG was quantified by the relative fluorescence intensity (RFI) of bound FITC-heparin. Values were expressed as a RFI ratio (RFI patient / RFI normal) and were 1.965±0.413 in HIT type II patients (n=36) and 1.064±0.162 in healthy controls (n=50, p<0.0001). The intra- and inter-assay coefficients of variation were 4.9 and 10.4%, respectively. The heparin-induced IgG FLIFA will be useful in individual and epidemiological studies in patients during treatment with heparin. The FLIFA technique offers an alternative, rapid and sensitive methodological approach for studies on the interaction between antigen-antibody or ligand-receptor. 
650 4 |a Antibody 
650 4 |a Fluorescence linked immunofiltration assay 
650 4 |a Heparin-induced thrombocytopenia 
650 4 |a Relative fluorescence intensity 
700 1 |a Huhle, Günter  |e VerfasserIn  |0 (DE-588)1077938500  |0 (DE-627)837763940  |0 (DE-576)173334431  |4 aut 
700 1 |a Malsch, Reinhard  |e VerfasserIn  |0 (DE-588)1258269597  |0 (DE-627)1804249149  |4 aut 
700 1 |a Hoffmann, Ursula  |d 1962-  |e VerfasserIn  |0 (DE-588)115587098  |0 (DE-627)691432406  |0 (DE-576)289964113  |4 aut 
700 1 |a Song, Xuehong  |d 1966-  |e VerfasserIn  |0 (DE-588)122397908  |0 (DE-627)705893464  |0 (DE-576)293252955  |4 aut 
700 1 |a Harenberg, Job  |d 1950-  |e VerfasserIn  |0 (DE-588)108825507  |0 (DE-627)655891498  |0 (DE-576)339563745  |4 aut 
773 0 8 |i Enthalten in  |t Journal of immunological methods  |d Amsterdam [u.a.] : Elsevier Science, 1971  |g 222(1999), 1, Seite 93-99  |h Online-Ressource  |w (DE-627)306659751  |w (DE-600)1500495-8  |w (DE-576)081986386  |x 1872-7905  |7 nnas  |a Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA) 
773 1 8 |g volume:222  |g year:1999  |g number:1  |g pages:93-99  |g extent:7  |a Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA) 
856 4 0 |u https://doi.org/10.1016/S0022-1759(98)00186-0  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.sciencedirect.com/science/article/pii/S0022175998001860  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20220524 
994 |a 1999 
998 |g 108825507  |a Harenberg, Job  |m 108825507:Harenberg, Job  |d 60000  |d 61000  |d 60000  |e 60000PH108825507  |e 61000PH108825507  |e 60000PH108825507  |k 0/60000/  |k 1/60000/61000/  |k 0/60000/  |p 6  |y j 
998 |g 122397908  |a Song, Xuehong  |m 122397908:Song, Xuehong  |d 60000  |d 61000  |e 60000PS122397908  |e 61000PS122397908  |k 0/60000/  |k 1/60000/61000/  |p 5 
998 |g 115587098  |a Hoffmann, Ursula  |m 115587098:Hoffmann, Ursula  |d 60000  |d 61000  |d 60000  |e 60000PH115587098  |e 61000PH115587098  |e 60000PH115587098  |k 0/60000/  |k 1/60000/61000/  |k 0/60000/  |p 4 
998 |g 1258269597  |a Malsch, Reinhard  |m 1258269597:Malsch, Reinhard  |d 60000  |d 61000  |e 60000PM1258269597  |e 61000PM1258269597  |k 0/60000/  |k 1/60000/61000/  |p 3 
998 |g 1077938500  |a Huhle, Günter  |m 1077938500:Huhle, Günter  |d 60000  |d 61000  |e 60000PH1077938500  |e 61000PH1077938500  |k 0/60000/  |k 1/60000/61000/  |p 2 
998 |g 120909707  |a Wang, Lianchun  |m 120909707:Wang, Lianchun  |d 60000  |d 61000  |e 60000PW120909707  |e 61000PW120909707  |k 0/60000/  |k 1/60000/61000/  |p 1  |x j 
999 |a KXP-PPN1804249459  |e 4139252227 
BIB |a Y 
SER |a journal 
JSO |a {"language":["eng"],"recId":"1804249459","note":["Available online 22 December 1998","Gesehen am 24.05.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"roleDisplay":"VerfasserIn","display":"Wang, Lianchun","role":"aut","family":"Wang","given":"Lianchun"},{"given":"Günter","family":"Huhle","role":"aut","roleDisplay":"VerfasserIn","display":"Huhle, Günter"},{"family":"Malsch","given":"Reinhard","display":"Malsch, Reinhard","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Hoffmann","given":"Ursula","display":"Hoffmann, Ursula","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Xuehong","family":"Song","role":"aut","roleDisplay":"VerfasserIn","display":"Song, Xuehong"},{"given":"Job","family":"Harenberg","role":"aut","display":"Harenberg, Job","roleDisplay":"VerfasserIn"}],"title":[{"title_sort":"Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA)","title":"Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA)"}],"relHost":[{"origin":[{"publisherPlace":"Amsterdam [u.a.]","dateIssuedDisp":"1971-","publisher":"Elsevier Science","dateIssuedKey":"1971"}],"id":{"eki":["306659751"],"zdb":["1500495-8"],"issn":["1872-7905"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Journal of immunological methods","subtitle":"JIM","title":"Journal of immunological methods"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA)Journal of immunological methods","note":["Gesehen am 30.01.20"],"recId":"306659751","language":["eng"],"pubHistory":["1.1971/72 -"],"part":{"year":"1999","pages":"93-99","issue":"1","text":"222(1999), 1, Seite 93-99","volume":"222","extent":"7"},"titleAlt":[{"title":"JIM"}]}],"physDesc":[{"extent":"7 S."}],"name":{"displayForm":["Lianchun Wang, Guenter Huhle, Reinhard Malsch, Ursula Hoffmann, Xuehong Song, Job Harenberg"]},"id":{"doi":["10.1016/S0022-1759(98)00186-0"],"eki":["1804249459"]},"origin":[{"dateIssuedKey":"1999","dateIssuedDisp":"1999"}]} 
SRT |a WANGLIANCHDETERMINAT1999 

Ähnliche Einträge