TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes
The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
19 July 2021
|
| In: |
Scientific reports
Year: 2021, Jahrgang: 11, Pages: 1-12 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-93958-3 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41598-021-93958-3 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-021-93958-3 
|
| Verfasserangaben: | Katherine Sattler, Ibrahim El-Battrawy, Lukas Cyganek, Siegfried Lang, Huan Lan, Xin Li, Zhihan Zhao, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiaobo Zhou and Ibrahim Akin |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 180534854X | ||
| 003 | DE-627 | ||
| 005 | 20240416193551.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220531s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41598-021-93958-3 |2 doi | |
| 035 | |a (DE-627)180534854X | ||
| 035 | |a (DE-599)KXP180534854X | ||
| 035 | |a (OCoLC)1341460645 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Sattler, Katherine |d 1976- |e VerfasserIn |0 (DE-588)129625884 |0 (DE-627)475212509 |0 (DE-576)297755153 |4 aut | |
| 245 | 1 | 0 | |a TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes |c Katherine Sattler, Ibrahim El-Battrawy, Lukas Cyganek, Siegfried Lang, Huan Lan, Xin Li, Zhihan Zhao, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiaobo Zhou and Ibrahim Akin |
| 264 | 1 | |c 19 July 2021 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published: 19 July 2021 | ||
| 500 | |a Gesehen am 31.05.2022 | ||
| 520 | |a The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), TRPV1 agonist capsaicin (CAP, 20 min) or the antagonist capsazepine (CPZ, 20 min). TRPV1 expression was studied by PCR and western blotting. TRPV1 internalization was analyzed by immunofluorescence. Interleukin-6 (IL-6) secretion and phosphorylation of JNK, p38 and ERK were determined by ELISA. TRPV1-associated ion channel current was measured by patch clamp. TRPV1-mRNA and -protein were expressed in hiPSC-CM. TRPV1 was localized in the plasma membrane. LPS significantly increased secretion of IL-6 by 2.3-fold, which was prevented by pre-incubation with CPZ. LPS induced TRPV1 internalization. Phosphorylation levels of ERK, p38 or JNK were not altered by TRPV1 stimulation or inhibition. LPS and IL-6 significantly lowered TRPV1-mediated ion channel current. TRPV1 mediates the LPS-induced inflammation in cardiomyocytes, associated with changes of cellular electrophysiology. LPS-induced inflammation results in TRPV1 internalization. Further studies have to examine the underlying pathways and the clinical relevance of these findings. | ||
| 650 | 4 | |a Cardiovascular biology | |
| 650 | 4 | |a Mechanisms of disease | |
| 700 | 1 | |a El-Battrawy, Ibrahim |d 1987- |e VerfasserIn |0 (DE-588)1033436917 |0 (DE-627)741219549 |0 (DE-576)38084673X |4 aut | |
| 700 | 1 | |a Cyganek, Lukas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lang, Siegfried |d 1955- |e VerfasserIn |0 (DE-588)1026112613 |0 (DE-627)726109290 |0 (DE-576)371365473 |4 aut | |
| 700 | 1 | |a Lan, Huan |e VerfasserIn |0 (DE-588)1138467820 |0 (DE-627)896044157 |0 (DE-576)49256826X |4 aut | |
| 700 | 1 | |a Li, Xin |d 1987- |e VerfasserIn |0 (DE-588)1242674128 |0 (DE-627)1772767840 |4 aut | |
| 700 | 1 | |a Zhao, Zhihan |d 1987- |e VerfasserIn |0 (DE-588)112352680X |0 (DE-627)876729960 |0 (DE-576)48177825X |4 aut | |
| 700 | 1 | |a Utikal, Jochen |d 1974- |e VerfasserIn |0 (DE-588)1026463750 |0 (DE-627)726765015 |0 (DE-576)371816580 |4 aut | |
| 700 | 1 | |a Wieland, Thomas |d 1960-2025 |e VerfasserIn |0 (DE-588)1033445908 |0 (DE-627)741233215 |0 (DE-576)381043339 |4 aut | |
| 700 | 1 | |a Borggrefe, Martin |e VerfasserIn |0 (DE-588)1025920546 |0 (DE-627)725574232 |0 (DE-576)370913426 |4 aut | |
| 700 | 1 | |a Zhou, Xiao-Bo |d 1958- |e VerfasserIn |0 (DE-588)1117113744 |0 (DE-627)871032457 |0 (DE-576)478766742 |4 aut | |
| 700 | 1 | |a Akın, Ibrahim |d 1978- |e VerfasserIn |0 (DE-588)132322293 |0 (DE-627)521039010 |0 (DE-576)299074366 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Springer Nature, 2011 |g 11(2021), Artikel-ID 14689, Seite 1-12 |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnas |a TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes |
| 773 | 1 | 8 | |g volume:11 |g year:2021 |g elocationid:14689 |g pages:1-12 |g extent:12 |a TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41598-021-93958-3 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41598-021-93958-3 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220531 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 132322293 |a Akın, Ibrahim |m 132322293:Akın, Ibrahim |d 60000 |d 61000 |e 60000PA132322293 |e 61000PA132322293 |k 0/60000/ |k 1/60000/61000/ |p 12 |y j | ||
| 998 | |g 1117113744 |a Zhou, Xiao-Bo |m 1117113744:Zhou, Xiao-Bo |d 60000 |d 61000 |e 60000PZ1117113744 |e 61000PZ1117113744 |k 0/60000/ |k 1/60000/61000/ |p 11 | ||
| 998 | |g 1025920546 |a Borggrefe, Martin |m 1025920546:Borggrefe, Martin |d 60000 |d 61000 |e 60000PB1025920546 |e 61000PB1025920546 |k 0/60000/ |k 1/60000/61000/ |p 10 | ||
| 998 | |g 1033445908 |a Wieland, Thomas |m 1033445908:Wieland, Thomas |d 60000 |d 65000 |e 60000PW1033445908 |e 65000PW1033445908 |k 0/60000/ |k 1/60000/65000/ |p 9 | ||
| 998 | |g 1026463750 |a Utikal, Jochen |m 1026463750:Utikal, Jochen |d 60000 |e 60000PU1026463750 |k 0/60000/ |p 8 | ||
| 998 | |g 112352680X |a Zhao, Zhihan |m 112352680X:Zhao, Zhihan |d 60000 |d 61000 |e 60000PZ112352680X |e 61000PZ112352680X |k 0/60000/ |k 1/60000/61000/ |p 7 | ||
| 998 | |g 1242674128 |a Li, Xin |m 1242674128:Li, Xin |d 60000 |d 61000 |e 60000PL1242674128 |e 61000PL1242674128 |k 0/60000/ |k 1/60000/61000/ |p 6 | ||
| 998 | |g 1138467820 |a Lan, Huan |m 1138467820:Lan, Huan |d 60000 |d 61000 |e 60000PL1138467820 |e 61000PL1138467820 |k 0/60000/ |k 1/60000/61000/ |p 5 | ||
| 998 | |g 1026112613 |a Lang, Siegfried |m 1026112613:Lang, Siegfried |d 60000 |d 61000 |e 60000PL1026112613 |e 61000PL1026112613 |k 0/60000/ |k 1/60000/61000/ |p 4 | ||
| 998 | |g 1033436917 |a El-Battrawy, Ibrahim |m 1033436917:El-Battrawy, Ibrahim |d 60000 |d 61000 |e 60000PE1033436917 |e 61000PE1033436917 |k 0/60000/ |k 1/60000/61000/ |p 2 | ||
| 998 | |g 129625884 |a Sattler, Katherine |m 129625884:Sattler, Katherine |d 60000 |d 61000 |e 60000PS129625884 |e 61000PS129625884 |k 0/60000/ |k 1/60000/61000/ |p 1 |x j | ||
| 999 | |a KXP-PPN180534854X |e 4141815780 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Katherine Sattler, Ibrahim El-Battrawy, Lukas Cyganek, Siegfried Lang, Huan Lan, Xin Li, Zhihan Zhao, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiaobo Zhou and Ibrahim Akin"]},"id":{"doi":["10.1038/s41598-021-93958-3"],"eki":["180534854X"]},"physDesc":[{"extent":"12 S."}],"recId":"180534854X","person":[{"display":"Sattler, Katherine","given":"Katherine","role":"aut","family":"Sattler"},{"display":"El-Battrawy, Ibrahim","family":"El-Battrawy","role":"aut","given":"Ibrahim"},{"display":"Cyganek, Lukas","role":"aut","given":"Lukas","family":"Cyganek"},{"display":"Lang, Siegfried","family":"Lang","role":"aut","given":"Siegfried"},{"display":"Lan, Huan","role":"aut","given":"Huan","family":"Lan"},{"given":"Xin","role":"aut","family":"Li","display":"Li, Xin"},{"display":"Zhao, Zhihan","role":"aut","given":"Zhihan","family":"Zhao"},{"family":"Utikal","given":"Jochen","role":"aut","display":"Utikal, Jochen"},{"display":"Wieland, Thomas","role":"aut","given":"Thomas","family":"Wieland"},{"display":"Borggrefe, Martin","role":"aut","given":"Martin","family":"Borggrefe"},{"display":"Zhou, Xiao-Bo","family":"Zhou","given":"Xiao-Bo","role":"aut"},{"family":"Akın","given":"Ibrahim","role":"aut","display":"Akın, Ibrahim"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"recId":"663366712","disp":"TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytesScientific reports","pubHistory":["1, article number 1 (2011)-"],"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"2011-","publisherPlace":"[London] ; London","publisher":"Springer Nature ; Nature Publishing Group"}],"note":["Gesehen am 12.07.24"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"part":{"year":"2021","pages":"1-12","extent":"12","text":"11(2021), Artikel-ID 14689, Seite 1-12","volume":"11"},"title":[{"title":"Scientific reports","title_sort":"Scientific reports"}],"id":{"zdb":["2615211-3"],"eki":["663366712"],"issn":["2045-2322"]}}],"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"19 July 2021"}],"note":["Published: 19 July 2021","Gesehen am 31.05.2022"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"title":[{"title":"TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes","title_sort":"TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes"}]} | ||
| SRT | |a SATTLERKATTRPV1ACTIV1920 | ||