ADCK2 knockdown affects the migration of melanoma cells via MYL6
Background: ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
20 February 2022
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| In: |
Cancers
Year: 2022, Jahrgang: 14, Heft: 4, Pages: 1-18 |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers14041071 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.3390/cancers14041071 Verlag, kostenfrei, Volltext: https://www.mdpi.com/2072-6694/14/4/1071 
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| Verfasserangaben: | Marlene Vierthaler, Qian Sun, Yiman Wang, Tamara Steinfass, Juliane Poelchen, Thomas Hielscher, Daniel Novak, Viktor Umansky, Jochen Utikal |
| Zusammenfassung: | Background: ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK2 in melanoma. Methods: The effect of ADCK2 on melanoma cell motility was evaluated by a scratch assay and a transwell invasion assay upon siRNA-mediated knockdown or stable overexpression of ADCK2. Results: We found that high levels of intratumoral ADCK2 and MYL6 are associated with a higher survival rate in melanoma patients. Knocking down ADCK2 resulted in enhanced cell migration of melanoma cells. Moreover, ADCK2-knockdown cells adopted a more dedifferentiated phenotype. A gene expression array revealed that the expression of ADCK2 correlated with the expressions of MYL6 and RAB2A. Knocking down MYL6 in ADCK2-overexpressing cells could abrogate the effect of ADCK2 overexpression and thus confirm the functional connection between ADCK2 and MYL6. Conclusion: ADCK2 affects melanoma cell motility, most probably via MYL6. Our results allow the conclusion that ADCK2 could act as a tumor suppressor in melanoma. |
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| Beschreibung: | This article belongs to the Special Issue Epidemiology and Biological Features of Melanoma Gesehen am 09.06.2022 |
| Beschreibung: | Online Resource |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers14041071 |