PDGF-BB induces expression of LTBP-1 but not TGF-β1 in a rat cirrhotic fat storing cell line
TGF-β, a profibrogenic cytokine is predominantly secreted as a latent molecule complexed with one of the latent TGF-β binding proteins (LTBP). Due to the proposed functions of LTBP-1 and -3 in regulating TGF-β-bioavailability and -activity, we investigated the effects of PDGF-BB and TGF-β1 on their...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2003
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| In: |
Growth factors
Year: 2003, Jahrgang: 21, Heft: 3/4, Pages: 121-130 |
| ISSN: | 1029-2292 |
| DOI: | 10.1080/08977190310001637224 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1080/08977190310001637224
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| Verfasserangaben: | Jens H. Westhoff, Iris Sawitza, Jorma Keski-Oja, Axel M. Gressner & Katja Breitkopf |
| Zusammenfassung: | TGF-β, a profibrogenic cytokine is predominantly secreted as a latent molecule complexed with one of the latent TGF-β binding proteins (LTBP). Due to the proposed functions of LTBP-1 and -3 in regulating TGF-β-bioavailability and -activity, we investigated the effects of PDGF-BB and TGF-β1 on their expression levels in Cirrhotic fat storing cells (CFSC). CFSC basally express LTBP-1 and -3 and TGF-β1. LTBP-1 colocalizes with LAP and the cells secrete some active TGF-β1. Promoter studies showed no strong induction of the LTBP-1 promoters after stimulation, although mRNA and protein levels were increased by PDGF-BB treatment without affecting TGF-β1 expression. Vice versa, TGF-β1 treatment did not alter LTBP-1 expression while an autocrine induction was found. Our data indicate that LTBP-1 but not TGF-β1 is induced by PDGF-BB and that TGF-β1 autoinduction does not affect the expression of LTBP-1. This divergent regulation may represent an important mechanism for modulation of TGF-β bioavailability. |
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| Beschreibung: | Elektronische Reproduktion der Druck-Ausgabe Published online: 07 Aug 2009 Gesehen am 23.06.2022 |
| Beschreibung: | Online Resource |
| ISSN: | 1029-2292 |
| DOI: | 10.1080/08977190310001637224 |