Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells?
Objective - To assess the effects of metformin on insulin-, IGF I-, and IGF II-receptor gene expression and Akt phosphorylation in decidualized human endometrial stromal cells (ESC) after stimulation with insulin, IGF I and II. - Study design - ESC were isolated from healthy, regularly cycling women...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
12 June 2011
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| In: |
European journal of obstetrics & gynecology and reproductive biology
Year: 2011, Jahrgang: 158, Heft: 2, Pages: 248-253 |
| ISSN: | 1872-7654 |
| DOI: | 10.1016/j.ejogrb.2011.05.012 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ejogrb.2011.05.012 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0301211511003046 
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| Verfasserangaben: | Edison Capp, Julia Jauckus, Helena von Eye Corleta, Bettina Toth, Thomas Strowitzki, Ariane Germeyer |
MARC
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| 245 | 1 | 0 | |a Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells? |c Edison Capp, Julia Jauckus, Helena von Eye Corleta, Bettina Toth, Thomas Strowitzki, Ariane Germeyer |
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| 520 | |a Objective - To assess the effects of metformin on insulin-, IGF I-, and IGF II-receptor gene expression and Akt phosphorylation in decidualized human endometrial stromal cells (ESC) after stimulation with insulin, IGF I and II. - Study design - ESC were isolated from healthy, regularly cycling women and after two passages decidualized with estrogen/progesterone±metformin. Cells were incubated with insulin, IGF I or IGF II for 1, 5, and 10min to assess Akt phosphorylation by Western blot. To investigate the insulin-, IGF I- and IGF II-receptor gene expression ESC were incubated with insulin, IGF I or IGF II for 6 and 24h. - Results - Insulin- and IGF I-receptor gene expression in ESC changed significantly after incubation with insulin, IGF I or IGF II. This was further augmented in metformin pretreated cells, while IGF II-receptor gene expression changed particularly after pretreatment with metformin. Akt phosphorylation peaked after 5min insulin, IGF I and IGF II stimulation in ESC in both control (control 0.08±0.03 vs. insulin 0.74±0.19, IGF I 0.68±0.22, IGF II 0.53±0.13, p<0.05) and metformin pretreated cells (control 0.03±0.01 vs. insulin 0.75±0.11, IGF I 0.74±0.15, IGF II 0.67±0.09, p<0.005). However, there was no significant difference between the control and metformin pretreated group. - Conclusion - Insulin, IGF I and IGF II lead to changes in their receptor gene expression and induced Akt phosphorylation in ESC. These effects were further highlighted in the presence of metformin. | ||
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| 650 | 4 | |a Endometrium | |
| 650 | 4 | |a IGF receptor | |
| 650 | 4 | |a Insulin receptor | |
| 650 | 4 | |a Metformin | |
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