Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis
Commonly used colorimetric detection applied to protein microarrays with enzymatic signal amplification leads to non-linear signal production upon increase in analyte concentration, thereby considerably limiting the range and accuracy of quantitative readout interpretation. To extend the detection r...
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| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
08 March 2011
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| In: |
Proteomics
Year: 2011, Jahrgang: 11, Heft: 10, Pages: 2129-2133 |
| ISSN: | 1615-9861 |
| DOI: | 10.1002/pmic.201000690 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/pmic.201000690 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201000690 
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| Verfasserangaben: | Pavlo Holenya, Igor Kitanovic, Florian Heigwer, and Stefan Wölfl |
| Zusammenfassung: | Commonly used colorimetric detection applied to protein microarrays with enzymatic signal amplification leads to non-linear signal production upon increase in analyte concentration, thereby considerably limiting the range and accuracy of quantitative readout interpretation. To extend the detection range, we developed a kinetic colorimetric detection protocol for the analysis of ELISA microarrays designed to measure multiple phosphorylated proteins using the platforms ArrayTube™ and ArrayStrip™. With our novel quantification approach, microarrays were calibrated over a broad concentration range spanning four orders of magnitude of analyte concentration with picomolar threshold. We used this design for the simultaneous quantitative measurement of 15 phosphorylated proteins on a single chip. |
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| Beschreibung: | Gesehen am 29.06.2022 |
| Beschreibung: | Online Resource |
| ISSN: | 1615-9861 |
| DOI: | 10.1002/pmic.201000690 |