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The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro

The helper component-proteinase (HC-Pro) is a multifunctional protein found among potyviruses. With respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1...

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Hauptverfasser: Jamous, Rana M. (VerfasserIn) , Boonrod, Kajohn (VerfasserIn) , Fuellgrabe, Marc W. (VerfasserIn) , Ali-Shtayeh, Mohammed S. (VerfasserIn) , Krczal, Gabi (VerfasserIn) , Wassenegger, Michael (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 01 September 2011
In: Journal of general virology
Year: 2011, Jahrgang: 92, Heft: 9, Pages: 2222-2226
ISSN:1465-2099
DOI:10.1099/vir.0.031534-0
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1099/vir.0.031534-0
Verlag, lizenzpflichtig, Volltext: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.031534-0
Volltext
Verfasserangaben:Rana M. Jamous, Kajohn Boonrod, Marc W. Fuellgrabe, Mohammed S. Ali-Shtayeh, Gabi Krczal and Michael Wassenegger
Beschreibung
Zusammenfassung:The helper component-proteinase (HC-Pro) is a multifunctional protein found among potyviruses. With respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity. There is indirect evidence showing that either transient or stable expression of HC-Pro in plants results in an increase of non-methylated small RNAs. Here, we demonstrated that recombinant Zucchini yellow mosaic virus (ZYMV) HC-Pro inhibited the methyltransferase activity of HEN1 in vitro. Moreover, we found that the HC-ProFINK mutant, which has lost small RNA-binding activity, inhibited HEN1 activity, while the truncated proteins and total soluble bacterial proteins did not. Using the ELISA-binding assay, we provided evidence that the HC-ProFRNK wild-type and HC-ProFINK both bound to HEN1, with HC-ProFRNK binding stronger than HC-ProFINK. Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of ZYMV HC-Pro were associated with HEN1 interaction.,
Beschreibung:Gesehen am 04.07.2022
Beschreibung:Online Resource
ISSN:1465-2099
DOI:10.1099/vir.0.031534-0

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