Serotonin receptor diversity in the human colon: expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E
Since the first description of 5-HT3 receptors more than 50 years ago, there has been speculation about the molecular basis of their receptor heterogeneity. We have cloned the genes encoding novel 5-HT3 subunits 5-HT3C, 5-HT3D, and 5-HT3E and have shown that these subunits are able to form functiona...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
The journal of comparative neurology
Year: 2011, Volume: 519, Issue: 3, Pages: 420-432 |
| ISSN: | 1096-9861 |
| DOI: | 10.1002/cne.22525 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/cne.22525 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.22525 
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| Author Notes: | Johannes Kapeller, Dorothee Möller, Felix Lasitschka, Frank Autschbach, Ruud Hovius, Gudrun Rappold, Michael Brüss, Michael D. Gershon, and Beate Niesler |
MARC
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| 245 | 1 | 0 | |a Serotonin receptor diversity in the human colon |b expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E |c Johannes Kapeller, Dorothee Möller, Felix Lasitschka, Frank Autschbach, Ruud Hovius, Gudrun Rappold, Michael Brüss, Michael D. Gershon, and Beate Niesler |
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| 520 | |a Since the first description of 5-HT3 receptors more than 50 years ago, there has been speculation about the molecular basis of their receptor heterogeneity. We have cloned the genes encoding novel 5-HT3 subunits 5-HT3C, 5-HT3D, and 5-HT3E and have shown that these subunits are able to form functional heteromeric receptors when coexpressed with the 5-HT3A subunit. However, whether these subunits are actually expressed in human tissue remained to be confirmed. In the current study, we performed immunocytochemistry to locate the 5-HT3A as well as the 5-HT3C, 5-HT3D, and 5-HT3E subunits within the human colon. Western blot analysis was used to confirm subunit expression, and RT-PCR was employed to detect transcripts encoding 5-HT3 receptor subunits in microdissected tissue samples. This investigation revealed, for the first time, that 5-HT3C, 5-HT3D, and 5-HT3E subunits are coexpressed with 5-HT3A in cell bodies of myenteric neurons. Furthermore, 5-HT3A and 5-HT3D were found to be expressed in submucosal plexus of the human large intestine. These data provide a strong basis for future studies of the roles that specific 5-HT3 receptor subtypes play in the function of the enteric and central nervous systems and the contribution that specific 5-HT3 receptors make to the pathophysiology of gastrointestinal disorders such as irritable bowel syndrome and dyspepsia. J. Comp. Neurol. 519:420-432, 2011. © 2010 Wiley-Liss, Inc. | ||
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