Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque
Despite the wide-spread use of antiseptics in dental practice and oral care products, there is little public awareness of potential risks associated with antiseptic resistance and potentially concomitant cross-resistance. Therefore, the aim of this study was to investigate potential phenotypic adapt...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
19 May 2022
|
| In: |
Antibiotics
Year: 2022, Volume: 11, Issue: 5, Pages: 1-18 |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics11050688 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/antibiotics11050688 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2079-6382/11/5/688 
|
| Author Notes: | David L. Auer, Xiaojun Mao, Annette Carola Anderson, Denise Muehler, Annette Wittmer, Christiane von Ohle, Diana Wolff, Cornelia Frese, Karl-Anton Hiller, Tim Maisch, Wolfgang Buchalla, Elmar Hellwig, Ali Al-Ahmad and Fabian Cieplik |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1810181577 | ||
| 003 | DE-627 | ||
| 005 | 20250109050536.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220714s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/antibiotics11050688 |2 doi | |
| 035 | |a (DE-627)1810181577 | ||
| 035 | |a (DE-599)KXP1810181577 | ||
| 035 | |a (OCoLC)1341463983 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Auer, David L. |e VerfasserIn |0 (DE-588)126249219X |0 (DE-627)1810182611 |4 aut | |
| 245 | 1 | 0 | |a Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque |c David L. Auer, Xiaojun Mao, Annette Carola Anderson, Denise Muehler, Annette Wittmer, Christiane von Ohle, Diana Wolff, Cornelia Frese, Karl-Anton Hiller, Tim Maisch, Wolfgang Buchalla, Elmar Hellwig, Ali Al-Ahmad and Fabian Cieplik |
| 264 | 1 | |c 19 May 2022 | |
| 300 | |a 18 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.07.2022 | ||
| 500 | |a This article belongs to the special issue "Antimicrobial strategies against oral pathogenic bacteria and biofilm" | ||
| 520 | |a Despite the wide-spread use of antiseptics in dental practice and oral care products, there is little public awareness of potential risks associated with antiseptic resistance and potentially concomitant cross-resistance. Therefore, the aim of this study was to investigate potential phenotypic adaptation in 177 clinical isolates of early colonizers of dental plaque (Streptococcus, Actinomyces, Rothia and Veillonella spp.) upon repeated exposure to subinhibitory concentrations of chlorhexidine digluconate (CHX) or cetylpyridinium chloride (CPC) over 10 passages using a modified microdilution method. Stability of phenotypic adaptation was re-evaluated after culture in antiseptic-free nutrient broth for 24 or 72 h. Strains showing 8-fold minimal inhibitory concentration (MIC)-increase were further examined regarding their biofilm formation capacity, phenotypic antibiotic resistance and presence of antibiotic resistance genes (ARGs). Eight-fold MIC-increases to CHX were detected in four Streptococcus isolates. These strains mostly exhibited significantly increased biofilm formation capacity compared to their respective wild-type strains. Phenotypic antibiotic resistance was detected to tetracycline and erythromycin, consistent with the detected ARGs. In conclusion, this study shows that clinical isolates of early colonizers of dental plaque can phenotypically adapt toward antiseptics such as CHX upon repeated exposure. The underlying mechanisms at genomic and transcriptomic levels need to be investigated in future studies. | ||
| 650 | 4 | |a adaptation | |
| 650 | 4 | |a antibiotic | |
| 650 | 4 | |a antiseptic | |
| 650 | 4 | |a biocide | |
| 650 | 4 | |a cetylpyridinium chloride | |
| 650 | 4 | |a chlorhexidine | |
| 650 | 4 | |a oral biofilm | |
| 650 | 4 | |a resistance | |
| 700 | 1 | |a Mao, Xiaojun |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Anderson, Annette C. |d 1972- |e VerfasserIn |0 (DE-588)1127259199 |0 (DE-627)881713120 |0 (DE-576)485162997 |4 aut | |
| 700 | 1 | |a Muehler, Denise |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wittmer, Annette |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ohle, Christiane von |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wolff, Diana |d 1976- |e VerfasserIn |0 (DE-588)128783176 |0 (DE-627)379683377 |0 (DE-576)302560351 |4 aut | |
| 700 | 1 | |a Frese, Cornelia |e VerfasserIn |0 (DE-588)1050990811 |0 (DE-627)785478027 |0 (DE-576)405442262 |4 aut | |
| 700 | 1 | |a Hiller, Karl-Anton |e VerfasserIn |4 aut | |
| 700 | 1 | |8 2\p |a Maisch, Tim |d 1970- |e VerfasserIn |0 (DE-588)123479606 |0 (DE-627)706314026 |0 (DE-576)185316166 |4 aut | |
| 700 | 1 | |a Buchalla, Wolfgang |e VerfasserIn |4 aut | |
| 700 | 1 | |8 3\p |a Hellwig, Elmar |d 1954- |e VerfasserIn |0 (DE-588)109961234 |0 (DE-627)58779237X |0 (DE-576)169338908 |4 aut | |
| 700 | 1 | |8 4\p |a Al-Ahmad, Ali |d 1966- |e VerfasserIn |0 (DE-588)120468557 |0 (DE-627)080693318 |0 (DE-576)292234392 |4 aut | |
| 700 | 1 | |8 5\p |a Cieplik, Fabian Alfred Aloi |d 1989- |e VerfasserIn |0 (DE-588)1051981352 |0 (DE-627)78732860X |0 (DE-576)407643494 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Antibiotics |d Basel : MDPI, 2012 |g 11(2022), 5, special issue, Artikel-ID 688, Seite 1-18 |h Online-Ressource |w (DE-627)726120596 |w (DE-600)2681345-2 |w (DE-576)371374081 |x 2079-6382 |7 nnas |a Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque |
| 773 | 1 | 8 | |g volume:11 |g year:2022 |g number:5 |g supplement:special issue |g elocationid:688 |g pages:1-18 |g extent:18 |a Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque |
| 856 | 4 | 0 | |u https://doi.org/10.3390/antibiotics11050688 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2079-6382/11/5/688 |x Verlag |z lizenzpflichtig |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 2\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 3\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 4\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 5\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20220714 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 1050990811 |a Frese, Cornelia |m 1050990811:Frese, Cornelia |d 910000 |d 910800 |e 910000PF1050990811 |e 910800PF1050990811 |k 0/910000/ |k 1/910000/910800/ |p 8 | ||
| 998 | |g 128783176 |a Wolff, Diana |m 128783176:Wolff, Diana |d 910000 |d 910800 |e 910000PW128783176 |e 910800PW128783176 |k 0/910000/ |k 1/910000/910800/ |p 7 | ||
| 999 | |a KXP-PPN1810181577 |e 4165546550 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Auer","roleDisplay":"VerfasserIn","role":"aut","given":"David L.","display":"Auer, David L."},{"family":"Mao","roleDisplay":"VerfasserIn","role":"aut","display":"Mao, Xiaojun","given":"Xiaojun"},{"given":"Annette C.","display":"Anderson, Annette C.","role":"aut","roleDisplay":"VerfasserIn","family":"Anderson"},{"display":"Muehler, Denise","given":"Denise","family":"Muehler","role":"aut","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Wittmer","display":"Wittmer, Annette","given":"Annette"},{"given":"Christiane von","display":"Ohle, Christiane von","family":"Ohle","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Wolff, Diana","given":"Diana","family":"Wolff","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","family":"Frese","display":"Frese, Cornelia","given":"Cornelia"},{"given":"Karl-Anton","display":"Hiller, Karl-Anton","family":"Hiller","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Tim","display":"Maisch, Tim","role":"aut","roleDisplay":"VerfasserIn","family":"Maisch"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Buchalla","display":"Buchalla, Wolfgang","given":"Wolfgang"},{"given":"Elmar","display":"Hellwig, Elmar","role":"aut","roleDisplay":"VerfasserIn","family":"Hellwig"},{"display":"Al-Ahmad, Ali","given":"Ali","family":"Al-Ahmad","role":"aut","roleDisplay":"VerfasserIn"},{"display":"Cieplik, Fabian Alfred Aloi","given":"Fabian Alfred Aloi","family":"Cieplik","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque","title_sort":"Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaque"}],"name":{"displayForm":["David L. Auer, Xiaojun Mao, Annette Carola Anderson, Denise Muehler, Annette Wittmer, Christiane von Ohle, Diana Wolff, Cornelia Frese, Karl-Anton Hiller, Tim Maisch, Wolfgang Buchalla, Elmar Hellwig, Ali Al-Ahmad and Fabian Cieplik"]},"origin":[{"dateIssuedDisp":"19 May 2022","dateIssuedKey":"2022"}],"note":["Gesehen am 14.07.2022","This article belongs to the special issue \"Antimicrobial strategies against oral pathogenic bacteria and biofilm\""],"type":{"media":"Online-Ressource","bibl":"article-journal"},"relHost":[{"title":[{"title":"Antibiotics","subtitle":"open access journal","title_sort":"Antibiotics"}],"disp":"Phenotypic adaptation to antiseptics and effects on biofilm formation capacity and antibiotic resistance in clinical isolates of early colonizers in dental plaqueAntibiotics","id":{"zdb":["2681345-2"],"eki":["726120596"],"issn":["2079-6382"]},"recId":"726120596","language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 12.09.12"],"type":{"bibl":"periodical","media":"Online-Ressource"},"part":{"extent":"18","pages":"1-18","volume":"11","issue":"5","year":"2022","text":"11(2022), 5, special issue, Artikel-ID 688, Seite 1-18"},"pubHistory":["1.2012 -"],"origin":[{"dateIssuedDisp":"2012-","dateIssuedKey":"2012","publisherPlace":"Basel","publisher":"MDPI"}]}],"language":["eng"],"physDesc":[{"extent":"18 S."}],"id":{"eki":["1810181577"],"doi":["10.3390/antibiotics11050688"]},"recId":"1810181577"} | ||
| SRT | |a AUERDAVIDLPHENOTYPIC1920 | ||