Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells
T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy f...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
[30 April 2011]
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| In: |
Immunology letters
Year: 2011, Jahrgang: 136, Heft: 1, Pages: 13-20 |
| ISSN: | 1879-0542 |
| DOI: | 10.1016/j.imlet.2010.11.005 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.imlet.2010.11.005 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0165247810002944 
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| Verfasserangaben: | Sabrina Hoffmann, Babak H. Hosseini, Markus Hecker, Ilia Louban, Nadja Bulbuc, Natalio Garbi, Guido H. Wabnitz, Yvonne Samstag, Joachim P. Spatz, Günter J. Hämmerling |
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| 245 | 1 | 0 | |a Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells |c Sabrina Hoffmann, Babak H. Hosseini, Markus Hecker, Ilia Louban, Nadja Bulbuc, Natalio Garbi, Guido H. Wabnitz, Yvonne Samstag, Joachim P. Spatz, Günter J. Hämmerling |
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| 520 | |a T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune synapse formation, as determined by LFA-1 and TCR enrichment at the interface of T-cell/APC conjugates using high throughput multispectral imaging flow cytometry. Together, these results suggest that ICAM-1/LFA-1 redistribution to the contact area is mainly responsible for development of strong interaction forces. High forces will keep T-cells and APCs in tight contact, thereby providing a platform for optimal interaction between TCRs and peptide-MHC complexes. | ||
| 650 | 4 | |a Antigen presentation | |
| 650 | 4 | |a Atomic force microscopy | |
| 650 | 4 | |a Cellular interaction forces | |
| 650 | 4 | |a Intercellular adhesion molecule-1 (ICAM-1) | |
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