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Imaging label-free intracellular structures by localisation microscopy

Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time...

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Hauptverfasser: Kaufmann, Rainer (VerfasserIn) , Müller, Patrick (VerfasserIn) , Hausmann, Michael (VerfasserIn) , Cremer, Christoph (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2011
In: Micron
Year: 2011, Jahrgang: 42, Heft: 4, Pages: 348-352
ISSN:1878-4291
DOI:10.1016/j.micron.2010.03.006
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.micron.2010.03.006
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0968432810000594
Volltext
Verfasserangaben:Rainer Kaufmann, Patrick Müller, Michael Hausmann, Christoph Cremer

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520 |a Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the λ/10 range (∼50nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions. 
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