The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation
The steady-state level of each mRNA in a cell is a balance between synthesis and degradation. Here, we use high-throughput RNA sequencing (RNASeq) to determine the relationship between mRNA degradation and mRNA abundance on a transcriptome-wide scale. The model organism used was the bloodstream form...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
September 26, 2011
|
| In: |
RNA
Year: 2011, Jahrgang: 17, Heft: 11, Pages: 2039-2047 |
| ISSN: | 1469-9001 |
| DOI: | 10.1261/rna.2837311 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1261/rna.2837311 Verlag, lizenzpflichtig, Volltext: http://rnajournal.cshlp.org/content/17/11/2039 
|
| Verfasserangaben: | Theresa Manful, Abeer Fadda, and Christine Clayton |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1813801169 | ||
| 003 | DE-627 | ||
| 005 | 20230710123001.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220809s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1261/rna.2837311 |2 doi | |
| 035 | |a (DE-627)1813801169 | ||
| 035 | |a (DE-599)KXP1813801169 | ||
| 035 | |a (OCoLC)1389793270 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Manful Gwira, Theresa |e VerfasserIn |0 (DE-588)1159580146 |0 (DE-627)1022257730 |0 (DE-576)505045974 |4 aut | |
| 245 | 1 | 4 | |a The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation |c Theresa Manful, Abeer Fadda, and Christine Clayton |
| 264 | 1 | |c September 26, 2011 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 09.08.2022 | ||
| 520 | |a The steady-state level of each mRNA in a cell is a balance between synthesis and degradation. Here, we use high-throughput RNA sequencing (RNASeq) to determine the relationship between mRNA degradation and mRNA abundance on a transcriptome-wide scale. The model organism used was the bloodstream form of Trypanosoma brucei, a protist that lacks regulation of RNA polymerase II initiation. The mRNA half-lives varied over two orders of magnitude, with a median half-life of 13 min for total (rRNA-depleted) mRNA. Data for poly(A)+ RNA yielded shorter half-lives than for total RNA, indicating that removal of the poly(A) tail was usually the first step in degradation. Depletion of the major 5′-3′ exoribonuclease, XRNA, resulted in the stabilization of most mRNAs with half-lives under 30 min. Thus, on a transcriptome-wide scale, degradation of most mRNAs is initiated by deadenylation. Trypanosome mRNA levels are strongly influenced by gene copy number and mRNA half-life: Very abundant mRNAs that are required throughout the life-cycle may be encoded by multicopy genes and have intermediate-to-long half-lives; those encoding ribosomal proteins, with one to two gene copies, are exceptionally stable. Developmentally regulated transcripts with a lower abundance in the bloodstream forms than the procyclic forms had half-lives around the median, whereas those with a higher abundance in the bloodstream forms than the procyclic forms, such as those encoding glycolytic enzymes, had longer half-lives. | ||
| 650 | 4 | |a mRNA decay | |
| 650 | 4 | |a polycistronic | |
| 650 | 4 | |a RNASeq | |
| 650 | 4 | |a Trypanosoma | |
| 650 | 4 | |a Xrn1 | |
| 700 | 1 | |a Fadda, Abeer |e VerfasserIn |0 (DE-588)1156723353 |0 (DE-627)1019664959 |0 (DE-576)502396938 |4 aut | |
| 700 | 1 | |a Clayton, Christine |e VerfasserIn |0 (DE-588)1098447158 |0 (DE-627)85763335X |0 (DE-576)469125624 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t RNA |d Cold Spring Harbor, NY : Laboratory Press, 1995 |g 17(2011), 11, Seite 2039-2047 |h Online-Ressource |w (DE-627)269758844 |w (DE-600)1475737-0 |w (DE-576)25220901X |x 1469-9001 |7 nnas |a The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation |
| 773 | 1 | 8 | |g volume:17 |g year:2011 |g number:11 |g pages:2039-2047 |g extent:9 |a The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation |
| 856 | 4 | 0 | |u https://doi.org/10.1261/rna.2837311 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://rnajournal.cshlp.org/content/17/11/2039 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220809 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1098447158 |a Clayton, Christine |m 1098447158:Clayton, Christine |d 700000 |d 706000 |e 700000PC1098447158 |e 706000PC1098447158 |k 0/700000/ |k 1/700000/706000/ |p 3 |y j | ||
| 998 | |g 1156723353 |a Fadda, Abeer |m 1156723353:Fadda, Abeer |d 700000 |d 706000 |e 700000PF1156723353 |e 706000PF1156723353 |k 0/700000/ |k 1/700000/706000/ |p 2 | ||
| 999 | |a KXP-PPN1813801169 |e 4177194522 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1813801169","language":["eng"],"note":["Gesehen am 09.08.2022"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation","title_sort":"role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradation"}],"person":[{"family":"Manful Gwira","given":"Theresa","roleDisplay":"VerfasserIn","display":"Manful Gwira, Theresa","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Fadda, Abeer","given":"Abeer","family":"Fadda"},{"given":"Christine","family":"Clayton","role":"aut","display":"Clayton, Christine","roleDisplay":"VerfasserIn"}],"relHost":[{"language":["eng"],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"RNA Society"}],"recId":"269758844","type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"The role of the 5′-3′ exoribonuclease XRNA in transcriptome-wide mRNA degradationRNA","note":["Gesehen am 12.09.2018"],"part":{"pages":"2039-2047","issue":"11","year":"2011","extent":"9","volume":"17","text":"17(2011), 11, Seite 2039-2047"},"pubHistory":["1.1995 -"],"title":[{"title":"RNA","title_sort":"RNA"}],"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["publ. on behalf of the RNA Society"]},"id":{"zdb":["1475737-0"],"eki":["269758844"],"issn":["1469-9001"]},"origin":[{"dateIssuedDisp":"1995-","publisher":"Laboratory Press ; HighWire Press","dateIssuedKey":"1995","publisherPlace":"Cold Spring Harbor, NY ; Stanford, Calif."}]}],"physDesc":[{"extent":"9 S."}],"id":{"eki":["1813801169"],"doi":["10.1261/rna.2837311"]},"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"September 26, 2011"}],"name":{"displayForm":["Theresa Manful, Abeer Fadda, and Christine Clayton"]}} | ||
| SRT | |a MANFULGWIRROLEOFTHE52620 | ||