Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer
BackgroundExosomes contribute to immunosuppression in head and neck squamous cell carcinoma (HNSCC), a tumor entity which lacks specific tumor biomarkers. Plasma-derived exosomes from HNSCC patients correlate with clinical parameters and have potential as liquid biopsy. Here, we investigate the carg...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 July 2022
|
| In: |
Frontiers in medicine
Year: 2022, Jahrgang: 9, Pages: 1-14 |
| ISSN: | 2296-858X |
| DOI: | 10.3389/fmed.2022.904295 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.3389/fmed.2022.904295 Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/articles/10.3389/fmed.2022.904295 
|
| Verfasserangaben: | Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth, Daphne Engelhardt, Simon Laban, Patrick J. Schuler, Thomas K. Hoffmann, Cornelia Brunner, Edwin K. Jackson and Marie-Nicole Theodoraki |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1815764627 | ||
| 003 | DE-627 | ||
| 005 | 20241015095200.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220905s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fmed.2022.904295 |2 doi | |
| 035 | |a (DE-627)1815764627 | ||
| 035 | |a (DE-599)KXP1815764627 | ||
| 035 | |a (OCoLC)1361697002 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hofmann, Linda |e VerfasserIn |0 (DE-588)1269160273 |0 (DE-627)1817833952 |4 aut | |
| 245 | 1 | 0 | |a Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer |c Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth, Daphne Engelhardt, Simon Laban, Patrick J. Schuler, Thomas K. Hoffmann, Cornelia Brunner, Edwin K. Jackson and Marie-Nicole Theodoraki |
| 264 | 1 | |c 11 July 2022 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.09.2022 | ||
| 520 | |a BackgroundExosomes contribute to immunosuppression in head and neck squamous cell carcinoma (HNSCC), a tumor entity which lacks specific tumor biomarkers. Plasma-derived exosomes from HNSCC patients correlate with clinical parameters and have potential as liquid biopsy. Here, we investigate the cargo and functional profile of saliva-derived exosomes from HNSCC patients and their potential as non-invasive biomarkers for disease detection and immunomodulation.MethodsExosomes were isolated from saliva of HNSCC patients (n = 21) and healthy donors (HD, n = 12) by differential ultracentrifugation. Surface values of immune checkpoints and tumor associated antigens on saliva-derived exosomes were analyzed by bead-based flow cytometry using CD63 capture. Upon co-incubation with saliva-derived exosomes, activity and proliferation of T cells were assessed by flow cytometry (CD69 expression, CFSE assay). Adenosine levels were measured by mass spectrometry after incubation of saliva-derived exosomes with exogenous ATP. miRNA profiling of saliva-derived exosomes was performed using the nCounter® SPRINT system.ResultsSaliva-derived, CD63-captured exosomes from HNSCC patients carried high amounts of CD44v3, PDL1 and CD39. Compared to plasma, saliva was rich in tumor-derived, CD44v3+ exosomes and poor in hematopoietic cell-derived, CD45+ exosomes. CD8+ T cell activity was attenuated by saliva-derived exosomes from HNSCC patients, while proliferation of CD4+ T cells was not affected. Further, saliva-derived exosomes produced high levels of immunosuppressive adenosine. 62 HD- and 31 HNSCC-exclusive miRNAs were identified. Samples were grouped in “Healthy” and “Cancer” based on their saliva-derived exosomal miRNA profile, which was further found to be involved in RAS/MAPK, NF-κB complex, Smad2/3, and IFN-α signaling.ConclusionsSaliva-derived exosomes from HNSCC patients were enriched in tumor-derived exosomes whose cargo and functional profile reflected an immunosuppressive TME. Surface values of CD44v3, PDL1 and CD39 on CD63-captured exosomes, adenosine production and the miRNA cargo of saliva-derived exosomes emerged as discriminators of disease and emphasized their potential as liquid biomarkers specific for HNSCC. | ||
| 700 | 1 | |a Medyany, Valentin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ezić, Jasmin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lotfi, Ramin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Niesler, Beate |e VerfasserIn |0 (DE-588)1066800693 |0 (DE-627)817949615 |0 (DE-576)426148452 |4 aut | |
| 700 | 1 | |a Röth, Ralph |e VerfasserIn |0 (DE-588)1068410221 |0 (DE-627)820235563 |0 (DE-576)427767059 |4 aut | |
| 700 | 1 | |a Engelhardt, Daphne |e VerfasserIn |4 aut | |
| 700 | 1 | |a Laban, Simon Andreas |d 1981- |e VerfasserIn |0 (DE-588)138995524 |0 (DE-627)703174983 |0 (DE-576)309849039 |4 aut | |
| 700 | 1 | |a Schuler, Patrick |d 1978- |e VerfasserIn |0 (DE-588)1057847127 |0 (DE-627)79530580X |0 (DE-576)413859304 |4 aut | |
| 700 | 1 | |a Hoffmann, Thomas K. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Brunner, Cornelia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jackson, Edwin K. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Theodoraki, Marie-Nicole |d 1989- |e VerfasserIn |0 (DE-588)1195460570 |0 (DE-627)1677500638 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in medicine |d Lausanne : Frontiers Media, 2014 |g 9(2022), Artikel-ID 904295, Seite 1-14 |h Online-Ressource |w (DE-627)789482991 |w (DE-600)2775999-4 |w (DE-576)408729597 |x 2296-858X |7 nnas |a Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer |
| 773 | 1 | 8 | |g volume:9 |g year:2022 |g elocationid:904295 |g pages:1-14 |g extent:14 |a Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fmed.2022.904295 |x Resolving-System |x Verlag |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/articles/10.3389/fmed.2022.904295 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220905 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 1057847127 |a Schuler, Patrick |m 1057847127:Schuler, Patrick |p 9 | ||
| 998 | |g 1068410221 |a Röth, Ralph |m 1068410221:Röth, Ralph |d 50000 |e 50000PR1068410221 |k 0/50000/ |p 6 | ||
| 998 | |g 1066800693 |a Niesler, Beate |m 1066800693:Niesler, Beate |d 910000 |d 911500 |d 50000 |e 910000PN1066800693 |e 911500PN1066800693 |e 50000PN1066800693 |k 0/910000/ |k 1/910000/911500/ |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN1815764627 |e 4184645631 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"display":"Hofmann, Linda","family":"Hofmann","role":"aut","given":"Linda"},{"given":"Valentin","role":"aut","family":"Medyany","display":"Medyany, Valentin"},{"family":"Ezić","display":"Ezić, Jasmin","role":"aut","given":"Jasmin"},{"family":"Lotfi","display":"Lotfi, Ramin","given":"Ramin","role":"aut"},{"family":"Niesler","display":"Niesler, Beate","role":"aut","given":"Beate"},{"family":"Röth","display":"Röth, Ralph","role":"aut","given":"Ralph"},{"role":"aut","given":"Daphne","display":"Engelhardt, Daphne","family":"Engelhardt"},{"display":"Laban, Simon Andreas","family":"Laban","role":"aut","given":"Simon Andreas"},{"role":"aut","given":"Patrick","display":"Schuler, Patrick","family":"Schuler"},{"display":"Hoffmann, Thomas K.","family":"Hoffmann","given":"Thomas K.","role":"aut"},{"display":"Brunner, Cornelia","family":"Brunner","role":"aut","given":"Cornelia"},{"display":"Jackson, Edwin K.","family":"Jackson","role":"aut","given":"Edwin K."},{"display":"Theodoraki, Marie-Nicole","family":"Theodoraki","given":"Marie-Nicole","role":"aut"}],"language":["eng"],"note":["Gesehen am 05.09.2022"],"title":[{"title_sort":"Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer","title":"Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancer"}],"origin":[{"dateIssuedKey":"2022","dateIssuedDisp":"11 July 2022"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1815764627","name":{"displayForm":["Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth, Daphne Engelhardt, Simon Laban, Patrick J. Schuler, Thomas K. Hoffmann, Cornelia Brunner, Edwin K. Jackson and Marie-Nicole Theodoraki"]},"physDesc":[{"extent":"14 S."}],"relHost":[{"disp":"Cargo and functional profile of saliva-derived exosomes reveal biomarkers specific for head and neck cancerFrontiers in medicine","part":{"volume":"9","extent":"14","text":"9(2022), Artikel-ID 904295, Seite 1-14","pages":"1-14","year":"2022"},"note":["Gesehen am 09.02.2021"],"titleAlt":[{"title":"FMED"},{"title":"Front. Med."}],"origin":[{"dateIssuedKey":"2014","publisher":"Frontiers Media","publisherPlace":"Lausanne","dateIssuedDisp":"2014-"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title_sort":"Frontiers in medicine","title":"Frontiers in medicine"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"recId":"789482991","pubHistory":["2014 -"],"id":{"eki":["789482991"],"issn":["2296-858X"],"zdb":["2775999-4"]}}],"id":{"doi":["10.3389/fmed.2022.904295"],"eki":["1815764627"]}} | ||
| SRT | |a HOFMANNLINCARGOANDFU1120 | ||