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Expanding the chemical scope of RNA: methyltransferases to site-specific alkynylation of RNA for click labeling

This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S -adenosyl- l -methionine was employed to enzymatically transfer a fi...

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Main Authors: Motorin, Yuri (Author) , Burhenne, Jürgen (Author) , Teimer, Roman (Author) , Koynov, Kaloian (Author) , Willnow, Sophie (Author) , Weinhold, Elmar (Author) , Helm, Mark (Author)
Format: Article (Journal)
Language:English
Published: 2011
In: Nucleic acids research
Year: 2011, Volume: 39, Issue: 5, Pages: 1943-1952
ISSN:1362-4962
DOI:10.1093/nar/gkq825
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gkq825
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Author Notes:Yuri Motorin, Jürgen Burhenne, Roman Teimer, Kaloian Koynov, Sophie Willnow, Elmar Weinhold and Mark Helm

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