Blockade of leukocyte haptokinesis and haptotaxis by ketoprofen, diclofenac and SC-560
BACKGROUND: Nonsteroidal anti-inflammatory drugs (NSAID) represent a one of the most widely used anti-inflammatory substances. Their anti-inflammatory effects are mainly based on inhibition of cyclooxygenase. The potential direct effect of NSAID on leukocyte migration was poorly investigated. Using...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
12 November 2011
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| In: |
BMC immunology
Year: 2011, Volume: 12, Pages: 1-9 |
| ISSN: | 1471-2172 |
| DOI: | 10.1186/1471-2172-12-64 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1186/1471-2172-12-64
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| Author Notes: | Saulius Paskauskas, Audrius Parseliunas, Vachtang Kerkadze, Rainer Nobiling, Jan Schmidt and Eduard Ryschich |
| Summary: | BACKGROUND: Nonsteroidal anti-inflammatory drugs (NSAID) represent a one of the most widely used anti-inflammatory substances. Their anti-inflammatory effects are mainly based on inhibition of cyclooxygenase. The potential direct effect of NSAID on leukocyte migration was poorly investigated. Using time-lapse microscopy and 96-well fluorescence-based assay, we studied the effect of three different NSAID, ketoprofen, diclofenac and SC-560, on leukocyte haptokinesis and haptotaxis in vivo and in vitro. - RESULTS: NSAID induced an immediate inhibiting effect on leukocyte migration both in vitro and in vivo. This effect was dose-dependent and was not restricted to a specific type of leukocytes. The inhibition of leukocyte migration by NSAID was partially re-stored after removal of inhibiting agent. Only complete blockade of leukocyte migration was accompanied by a strong reduction of [Ca(2+)]i. - CONCLUSIONS: NSAID strongly supress leukocyte migration. The results of the present study may have important clinical implications since blockade of leukocyte migration can be achieved after topical application of NSAID. |
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| Item Description: | Gesehen am 22.09.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1471-2172 |
| DOI: | 10.1186/1471-2172-12-64 |