Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2
BackgroundStudies to verify correlations between phenotypes and genotypes of herpes simplex virus (HSV) are an important tool to establish a database of resistance-associated mutations.MethodsIn this study, 32 acyclovir (ACV)-resistant clinical HSV-1 and 4 ACV-resistant clinical HSV-2 isolates were...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 1, 2011
|
| In: |
Antiviral therapy
Year: 2011, Volume: 16, Issue: 8, Pages: 1297-1308 |
| ISSN: | 2040-2058 |
| DOI: | 10.3851/IMP1870 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3851/IMP1870
|
| Author Notes: | Andreas Sauerbrei, Kathrin Bohn, Albert Heim, Jörg Hofmann, Benedikt Weißbrich, Paul Schnitzler, Dieter Hoffmann, Roland Zell, Gerhard Jahn, Peter Wutzler, Klaus Hamprecht |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1818141884 | ||
| 003 | DE-627 | ||
| 005 | 20230710112246.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221006s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3851/IMP1870 |2 doi | |
| 035 | |a (DE-627)1818141884 | ||
| 035 | |a (DE-599)KXP1818141884 | ||
| 035 | |a (OCoLC)1389779535 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Sauerbrei, Andreas |e VerfasserIn |0 (DE-588)109783409 |0 (DE-627)592607100 |0 (DE-576)303538066 |4 aut | |
| 245 | 1 | 0 | |a Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2 |c Andreas Sauerbrei, Kathrin Bohn, Albert Heim, Jörg Hofmann, Benedikt Weißbrich, Paul Schnitzler, Dieter Hoffmann, Roland Zell, Gerhard Jahn, Peter Wutzler, Klaus Hamprecht |
| 246 | 3 | 0 | |a one two |
| 264 | 1 | |c November 1, 2011 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.10.2022 | ||
| 520 | |a BackgroundStudies to verify correlations between phenotypes and genotypes of herpes simplex virus (HSV) are an important tool to establish a database of resistance-associated mutations.MethodsIn this study, 32 acyclovir (ACV)-resistant clinical HSV-1 and 4 ACV-resistant clinical HSV-2 isolates were examined in parallel by both phenotypic and genotypic resistance testing. Additionally, five non-viable HSV-1 strains and two non-viable HSV-2 strains with clinical resistance were included in genotypic resistance analysis.ResultsAll ACV-resistant HSV isolates showed cross-resistance to brivudin and penciclovir, and were sensitive to foscarnet and cidofovir. Acyclovir resistance was assigned to frameshift and single non-synonymous mutations of the thymidine kinase (TK) gene in 32 out of 37 HSV-1 strains and in 4 out of 6 HSV-2 strains. In three HSV-1 isolates, there were resistance-associated amino acid substitutions of the DNA polymerase (pol). Six substitutions in the TK and two in the DNA pol gene could not be attributed without doubt to either ACV resistance or natural gene polymorphism. Altogether, 10 resistance-related mutations in the TK and 1 in the DNA pol gene have not been reported previously.ConclusionsThe novel non-synonymous mutations found in this study enrich the knowledge about the genetic alterations of TK and DNA pol genes in ACV-resistant clinical HSV strains. Together with data from the literature, the findings justify the generation of a HSV database that contains resistance mutations associated with ACV resistance phenotype. | ||
| 700 | 1 | |a Bohn, Kathrin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Heim, Albert |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hofmann, Jörg |e VerfasserIn |4 aut | |
| 700 | 1 | |a Weißbrich, Benedikt |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schnitzler, Paul |d 1959- |e VerfasserIn |0 (DE-588)1022905643 |0 (DE-627)717329798 |0 (DE-576)366155695 |4 aut | |
| 700 | 1 | |a Hoffmann, Dieter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Zell, Roland |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jahn, Gerhard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wutzler, Peter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hamprecht, Klaus |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Antiviral therapy |d London : Sage, 1998 |g 16(2011), 8, Seite 1297-1308 |h Online-Ressource |w (DE-627)369083024 |w (DE-600)2118396-X |w (DE-576)347267513 |x 2040-2058 |7 nnas |a Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2 |
| 773 | 1 | 8 | |g volume:16 |g year:2011 |g number:8 |g pages:1297-1308 |g extent:12 |a Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2 |
| 856 | 4 | 0 | |u https://doi.org/10.3851/IMP1870 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221006 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1022905643 |a Schnitzler, Paul |m 1022905643:Schnitzler, Paul |d 910000 |d 911700 |d 50000 |e 910000PS1022905643 |e 911700PS1022905643 |e 50000PS1022905643 |k 0/910000/ |k 1/910000/911700/ |k 0/50000/ |p 6 | ||
| 999 | |a KXP-PPN1818141884 |e 4194817001 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1818141884","relHost":[{"disp":"Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2Antiviral therapy","physDesc":[{"extent":"Online-Ressource"}],"part":{"text":"16(2011), 8, Seite 1297-1308","year":"2011","pages":"1297-1308","issue":"8","volume":"16","extent":"12"},"title":[{"title_sort":"Antiviral therapy","subtitle":"an official publication of the International Society for Antiviral Research","title":"Antiviral therapy"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"issn":["2040-2058"],"zdb":["2118396-X"],"eki":["369083024"]},"language":["eng"],"recId":"369083024","pubHistory":["Nachgewiesen 3.1998 -"],"origin":[{"publisherPlace":"London ; London","dateIssuedKey":"1998","dateIssuedDisp":"1998-","publisher":"Sage ; International Medical Press"}],"note":["Gesehen am 16.03.2022"]}],"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"November 1, 2011"}],"note":["Gesehen am 06.10.2022"],"person":[{"role":"aut","display":"Sauerbrei, Andreas","given":"Andreas","family":"Sauerbrei"},{"family":"Bohn","given":"Kathrin","display":"Bohn, Kathrin","role":"aut"},{"role":"aut","given":"Albert","display":"Heim, Albert","family":"Heim"},{"family":"Hofmann","given":"Jörg","display":"Hofmann, Jörg","role":"aut"},{"family":"Weißbrich","role":"aut","given":"Benedikt","display":"Weißbrich, Benedikt"},{"display":"Schnitzler, Paul","given":"Paul","role":"aut","family":"Schnitzler"},{"display":"Hoffmann, Dieter","given":"Dieter","role":"aut","family":"Hoffmann"},{"family":"Zell","role":"aut","display":"Zell, Roland","given":"Roland"},{"family":"Jahn","display":"Jahn, Gerhard","given":"Gerhard","role":"aut"},{"role":"aut","given":"Peter","display":"Wutzler, Peter","family":"Wutzler"},{"family":"Hamprecht","given":"Klaus","display":"Hamprecht, Klaus","role":"aut"}],"title":[{"title_sort":"Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2","title":"Novel resistance-associated mutations of thymidine kinase and dna polymerase genes of herpes simplex virus type 1 and type 2"}],"physDesc":[{"extent":"12 S."}],"id":{"doi":["10.3851/IMP1870"],"eki":["1818141884"]},"language":["eng"],"name":{"displayForm":["Andreas Sauerbrei, Kathrin Bohn, Albert Heim, Jörg Hofmann, Benedikt Weißbrich, Paul Schnitzler, Dieter Hoffmann, Roland Zell, Gerhard Jahn, Peter Wutzler, Klaus Hamprecht"]},"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a SAUERBREIANOVELRESIS1201 | ||