Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo
As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting, and folding factors. Here, we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Es...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 December 2011
|
| In: |
Cell
Year: 2011, Jahrgang: 147, Heft: 6, Pages: 1295-1308 |
| ISSN: | 1097-4172 |
| DOI: | 10.1016/j.cell.2011.10.044 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cell.2011.10.044 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0092867411013481 
|
| Verfasserangaben: | Eugene Oh, Annemarie H. Becker, Arzu Sandikci, Damon Huber, Rachna Chaba, Felix Gloge, Robert J. Nichols, Athanasios Typas, Carol A. Gross, Günter Kramer, Jonathan S. Weissman, and Bernd Bukau |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1818764849 | ||
| 003 | DE-627 | ||
| 005 | 20240326080204.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221012s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.cell.2011.10.044 |2 doi | |
| 035 | |a (DE-627)1818764849 | ||
| 035 | |a (DE-599)KXP1818764849 | ||
| 035 | |a (OCoLC)1389768580 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Oh, Eugene |e VerfasserIn |0 (DE-588)1270109804 |0 (DE-627)1818816806 |4 aut | |
| 245 | 1 | 0 | |a Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo |c Eugene Oh, Annemarie H. Becker, Arzu Sandikci, Damon Huber, Rachna Chaba, Felix Gloge, Robert J. Nichols, Athanasios Typas, Carol A. Gross, Günter Kramer, Jonathan S. Weissman, and Bernd Bukau |
| 264 | 1 | |c 8 December 2011 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.10.2022 | ||
| 520 | |a As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting, and folding factors. Here, we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Escherichia coli chaperone trigger factor (TF) reveal that, though TF can interact with many polypeptides, β-barrel outer-membrane proteins are the most prominent substrates. Loss of TF leads to broad outer-membrane defects and premature, cotranslational protein translocation. Whereas in vitro studies suggested that TF is prebound to ribosomes waiting for polypeptides to emerge from the exit channel, we find that in vivo TF engages ribosomes only after ∼100 amino acids are translated. Moreover, excess TF interferes with cotranslational removal of the N-terminal formyl methionine. Our studies support a triaging model in which proper protein biogenesis relies on the fine-tuned, sequential engagement of processing, targeting, and folding factors. - PaperClip | ||
| 700 | 1 | |a Becker, Annemarie |e VerfasserIn |0 (DE-588)1029442436 |0 (DE-627)733557635 |0 (DE-576)377295647 |4 aut | |
| 700 | 1 | |a Sandıkcı, Arzu |e VerfasserIn |0 (DE-588)1019510323 |0 (DE-627)690927940 |0 (DE-576)359510604 |4 aut | |
| 700 | 1 | |a Huber, Damon |e VerfasserIn |0 (DE-588)1166888002 |0 (DE-627)1030763380 |0 (DE-576)510944647 |4 aut | |
| 700 | 1 | |a Chaba, Rachna |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gloge, Felix |e VerfasserIn |0 (DE-588)1044930896 |0 (DE-627)773124101 |0 (DE-576)398222711 |4 aut | |
| 700 | 1 | |a Nichols, Robert J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Typas, Athanasios |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gross, Carol A. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kramer, Günter |e VerfasserIn |0 (DE-588)1060239582 |0 (DE-627)799340294 |0 (DE-576)416341136 |4 aut | |
| 700 | 1 | |a Weissman, Jonathan S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bukau, Bernd |d 1954- |e VerfasserIn |0 (DE-588)143209019 |0 (DE-627)643892575 |0 (DE-576)335649270 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell |d [Cambridge, Mass.] : Cell Press, 1974 |g 147(2011), 6, Seite 1295-1308 |h Online-Ressource |w (DE-627)320416127 |w (DE-600)2001951-8 |w (DE-576)090881370 |x 1097-4172 |7 nnas |a Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo |
| 773 | 1 | 8 | |g volume:147 |g year:2011 |g number:6 |g pages:1295-1308 |g extent:14 |a Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.cell.2011.10.044 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0092867411013481 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221012 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 143209019 |a Bukau, Bernd |m 143209019:Bukau, Bernd |d 700000 |d 706000 |e 700000PB143209019 |e 706000PB143209019 |k 0/700000/ |k 1/700000/706000/ |p 12 |y j | ||
| 998 | |g 1060239582 |a Kramer, Günter |m 1060239582:Kramer, Günter |d 700000 |d 706000 |e 700000PK1060239582 |e 706000PK1060239582 |k 0/700000/ |k 1/700000/706000/ |p 10 | ||
| 998 | |g 1044930896 |a Gloge, Felix |m 1044930896:Gloge, Felix |d 140000 |e 140000PG1044930896 |k 0/140000/ |p 6 | ||
| 998 | |g 1166888002 |a Huber, Damon |m 1166888002:Huber, Damon |d 700000 |d 706000 |e 700000PH1166888002 |e 706000PH1166888002 |k 0/700000/ |k 1/700000/706000/ |p 4 | ||
| 998 | |g 1019510323 |a Sandıkcı, Arzu |m 1019510323:Sandıkcı, Arzu |d 140000 |e 140000PS1019510323 |k 0/140000/ |p 3 | ||
| 998 | |g 1029442436 |a Becker, Annemarie |m 1029442436:Becker, Annemarie |d 140000 |e 140000PB1029442436 |k 0/140000/ |p 2 | ||
| 999 | |a KXP-PPN1818764849 |e 4196392709 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"note":["Gesehen am 12.10.2022"],"person":[{"family":"Oh","role":"aut","display":"Oh, Eugene","given":"Eugene"},{"given":"Annemarie","role":"aut","family":"Becker","display":"Becker, Annemarie"},{"role":"aut","family":"Sandıkcı","display":"Sandıkcı, Arzu","given":"Arzu"},{"family":"Huber","role":"aut","display":"Huber, Damon","given":"Damon"},{"given":"Rachna","role":"aut","family":"Chaba","display":"Chaba, Rachna"},{"given":"Felix","family":"Gloge","role":"aut","display":"Gloge, Felix"},{"role":"aut","family":"Nichols","display":"Nichols, Robert J.","given":"Robert J."},{"given":"Athanasios","role":"aut","family":"Typas","display":"Typas, Athanasios"},{"given":"Carol A.","family":"Gross","role":"aut","display":"Gross, Carol A."},{"given":"Günter","display":"Kramer, Günter","role":"aut","family":"Kramer"},{"family":"Weissman","role":"aut","display":"Weissman, Jonathan S.","given":"Jonathan S."},{"given":"Bernd","family":"Bukau","role":"aut","display":"Bukau, Bernd"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1818764849","id":{"doi":["10.1016/j.cell.2011.10.044"],"eki":["1818764849"]},"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"8 December 2011"}],"name":{"displayForm":["Eugene Oh, Annemarie H. Becker, Arzu Sandikci, Damon Huber, Rachna Chaba, Felix Gloge, Robert J. Nichols, Athanasios Typas, Carol A. Gross, Günter Kramer, Jonathan S. Weissman, and Bernd Bukau"]},"relHost":[{"title":[{"title":"Cell","title_sort":"Cell"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"14","issue":"6","year":"2011","pages":"1295-1308","volume":"147","text":"147(2011), 6, Seite 1295-1308"},"language":["eng"],"note":["Gesehen am 11.03.24"],"pubHistory":["1.1974 -"],"origin":[{"publisher":"Cell Press ; Elsevier","dateIssuedDisp":"1974-","publisherPlace":"[Cambridge, Mass.] ; New York, NY","dateIssuedKey":"1974"}],"disp":"Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivoCell","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"320416127","id":{"issn":["1097-4172"],"eki":["320416127"],"zdb":["2001951-8"]}}],"title":[{"title_sort":"Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo","title":"Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo"}],"physDesc":[{"extent":"14 S."}]} | ||
| SRT | |a OHEUGENEBESELECTIVER8201 | ||