Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation
Stimulation of CD95/Fas/APO-1 results in the induction of both apoptotic and non-apoptotic signaling pathways. The processes regulating these two opposing pathways have not been thoroughly elucidated to date. In this study, using quantitative immunoblots, imaging, and mathematical modeling, we addre...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
6 October 2011
|
| In: |
Cell death & disease
Year: 2011, Jahrgang: 2, Heft: 10, Pages: 1-10 |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/cddis.2011.93 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/cddis.2011.93 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/cddis201193 
|
| Verfasserangaben: | A.M.M. Kober, S. Legewie, C. Pforr, N. Fricker, R. Eils, P.H. Krammer and I.N. Lavrik |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1819005054 | ||
| 003 | DE-627 | ||
| 005 | 20230710110753.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221017s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/cddis.2011.93 |2 doi | |
| 035 | |a (DE-627)1819005054 | ||
| 035 | |a (DE-599)KXP1819005054 | ||
| 035 | |a (OCoLC)1389766713 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kober, A. M. M. |e VerfasserIn |4 aut | |
| 245 | 1 | 0 | |a Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation |c A.M.M. Kober, S. Legewie, C. Pforr, N. Fricker, R. Eils, P.H. Krammer and I.N. Lavrik |
| 264 | 1 | |c 6 October 2011 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.10.2022 | ||
| 520 | |a Stimulation of CD95/Fas/APO-1 results in the induction of both apoptotic and non-apoptotic signaling pathways. The processes regulating these two opposing pathways have not been thoroughly elucidated to date. In this study, using quantitative immunoblots, imaging, and mathematical modeling, we addressed the dynamics of the DED proteins of the death-inducing signaling complex (DISC), procaspase-8, and cellular FLICE inhibitory proteins (c-FLIPs) to the onset of CD95-mediated ERK1/2 and p38 mitogen-activated protein kinase (MAPK) activation. We found that CD95 DISC-induced caspase-8 activity is important for the initiation of ERK1/2 and p38 MAPK activation. The long c-FLIP isoform, c-FLIPL, and the short c-FLIP isoform, c-FLIPR, inhibited MAPK induction by blocking caspase-8 processing at the DISC. Furthermore, we built a mathematical model describing CD95 DISC-mediated MAPK activation and apoptosis. The model quantitatively defined the dynamics of DED proteins, procaspase-8, and c-FLIP, which lead to caspase-8 activation and induction of apoptotic and non-apoptotic signaling pathways. In conclusion, the combination of biochemical analysis with mathematical modeling provides evidence for an important role of caspase-8 in CD95-mediated activation of MAPKs, while c-FLIP exerts a regulatory function in this process. | ||
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Applied mathematics | |
| 650 | 4 | |a Biochemical assays | |
| 650 | 4 | |a Cell signalling | |
| 700 | 1 | |a Legewie, Stefan |d 1977- |e VerfasserIn |0 (DE-588)140387471 |0 (DE-627)618263772 |0 (DE-576)316676799 |4 aut | |
| 700 | 1 | |a Pforr, Carina |e VerfasserIn |0 (DE-588)141251727 |0 (DE-627)626163714 |0 (DE-576)32299702X |4 aut | |
| 700 | 1 | |a Fricker, Nicolai Benjamin |e VerfasserIn |0 (DE-588)1020321245 |0 (DE-627)691179573 |0 (DE-576)360146163 |4 aut | |
| 700 | 1 | |a Eils, Roland |d 1965- |e VerfasserIn |0 (DE-588)1020648287 |0 (DE-627)691291705 |0 (DE-576)361718195 |4 aut | |
| 700 | 1 | |a Krammer, Peter H. |d 1946- |e VerfasserIn |0 (DE-588)121766373 |0 (DE-627)081520255 |0 (DE-576)292873417 |4 aut | |
| 700 | 1 | |a Lavrik, Inna N. |e VerfasserIn |0 (DE-588)107529312X |0 (DE-627)833293869 |0 (DE-576)443375232 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell death & disease |d London [u.a.] : Nature Publishing Group, 2010 |g 2(2011), 10, Artikel-ID e212, Seite 1-10 |h Online-Ressource |w (DE-627)620145250 |w (DE-600)2541626-1 |w (DE-576)319429059 |x 2041-4889 |7 nnas |a Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation |
| 773 | 1 | 8 | |g volume:2 |g year:2011 |g number:10 |g elocationid:e212 |g pages:1-10 |g extent:10 |a Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation |
| 856 | 4 | 0 | |u https://doi.org/10.1038/cddis.2011.93 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/cddis201193 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221017 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 107529312X |a Lavrik, Inna N. |m 107529312X:Lavrik, Inna N. |d 50000 |d 700000 |d 716000 |e 50000PL107529312X |e 700000PL107529312X |e 716000PL107529312X |k 0/50000/ |k 0/700000/ |k 1/700000/716000/ |p 7 |y j | ||
| 998 | |g 121766373 |a Krammer, Peter H. |m 121766373:Krammer, Peter H. |d 50000 |e 50000PK121766373 |k 0/50000/ |p 6 | ||
| 998 | |g 1020648287 |a Eils, Roland |m 1020648287:Eils, Roland |d 160000 |d 160100 |d 700000 |d 716000 |e 160000PE1020648287 |e 160100PE1020648287 |e 700000PE1020648287 |e 716000PE1020648287 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |p 5 | ||
| 998 | |g 1020321245 |a Fricker, Nicolai Benjamin |m 1020321245:Fricker, Nicolai Benjamin |d 700000 |d 716000 |e 700000PF1020321245 |e 716000PF1020321245 |k 0/700000/ |k 1/700000/716000/ |p 4 | ||
| 998 | |g 140387471 |a Legewie, Stefan |m 140387471:Legewie, Stefan |d 700000 |d 716000 |e 700000PL140387471 |e 716000PL140387471 |k 0/700000/ |k 1/700000/716000/ |p 2 | ||
| 999 | |a KXP-PPN1819005054 |e 4197905343 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 17.10.2022"],"relHost":[{"recId":"620145250","disp":"Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activationCell death & disease","id":{"eki":["620145250"],"issn":["2041-4889"],"zdb":["2541626-1"]},"origin":[{"dateIssuedDisp":"2010-","dateIssuedKey":"2010","publisher":"Nature Publishing Group","publisherPlace":"London [u.a.]"}],"part":{"issue":"10","year":"2011","volume":"2","pages":"1-10","extent":"10","text":"2(2011), 10, Artikel-ID e212, Seite 1-10"},"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 26.02.10"],"titleAlt":[{"title":"Cell death and disease"}],"language":["eng"],"pubHistory":["1.2010 -"],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Cell death & disease","title_sort":"Cell death & disease"}]}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"physDesc":[{"extent":"10 S."}],"title":[{"title":"Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation","title_sort":"Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation"}],"person":[{"display":"Kober, A. M. M.","given":"A. M. M.","role":"aut","family":"Kober"},{"role":"aut","given":"Stefan","family":"Legewie","display":"Legewie, Stefan"},{"display":"Pforr, Carina","role":"aut","given":"Carina","family":"Pforr"},{"display":"Fricker, Nicolai Benjamin","given":"Nicolai Benjamin","role":"aut","family":"Fricker"},{"family":"Eils","given":"Roland","role":"aut","display":"Eils, Roland"},{"display":"Krammer, Peter H.","given":"Peter H.","role":"aut","family":"Krammer"},{"display":"Lavrik, Inna N.","family":"Lavrik","role":"aut","given":"Inna N."}],"recId":"1819005054","name":{"displayForm":["A.M.M. Kober, S. Legewie, C. Pforr, N. Fricker, R. Eils, P.H. Krammer and I.N. Lavrik"]},"origin":[{"dateIssuedDisp":"6 October 2011","dateIssuedKey":"2011"}],"id":{"eki":["1819005054"],"doi":["10.1038/cddis.2011.93"]}} | ||
| SRT | |a KOBERAMMLECASPASE8AC6201 | ||