Far-field optical nanoscopy with reduced number of state transition cycles
We report on a method to reduce the number of state transition cycles that a molecule undergoes in far-field optical nanoscopy of the RESOLFT type, i.e. concepts relying on saturable (fluorescence) state transitions induced by a spatially modulated light pattern. The method is exemplified for stimul...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 Mar 2011
|
| In: |
Optics express
Year: 2011, Jahrgang: 19, Heft: 6, Pages: 5644-5657 |
| ISSN: | 1094-4087 |
| DOI: | 10.1364/OE.19.005644 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1364/OE.19.005644 Verlag, lizenzpflichtig, Volltext: https://opg.optica.org/oe/abstract.cfm?uri=oe-19-6-5644 
|
| Verfasserangaben: | Thorsten Staudt, Andreas Engler, Eva Rittweger, Benjamin Harke, Johann Engelhardt, and Stefan W. Hell |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 181961249X | ||
| 003 | DE-627 | ||
| 005 | 20230710110318.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221021s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1364/OE.19.005644 |2 doi | |
| 035 | |a (DE-627)181961249X | ||
| 035 | |a (DE-599)KXP181961249X | ||
| 035 | |a (OCoLC)1389765703 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 29 |2 sdnb | ||
| 100 | 1 | |a Staudt, Thorsten |d 1980- |e VerfasserIn |0 (DE-588)1024101053 |0 (DE-627)719127548 |0 (DE-576)367928280 |4 aut | |
| 245 | 1 | 0 | |a Far-field optical nanoscopy with reduced number of state transition cycles |c Thorsten Staudt, Andreas Engler, Eva Rittweger, Benjamin Harke, Johann Engelhardt, and Stefan W. Hell |
| 264 | 1 | |c 11 Mar 2011 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 21.10.2022 | ||
| 520 | |a We report on a method to reduce the number of state transition cycles that a molecule undergoes in far-field optical nanoscopy of the RESOLFT type, i.e. concepts relying on saturable (fluorescence) state transitions induced by a spatially modulated light pattern. The method is exemplified for stimulated emission depletion (STED) microscopy which uses stimulated emission to transiently switch off the capability of fluorophores to fluoresce. By switching fluorophores off only if there is an adjacent fluorescent feature to be recorded, the method reduces the number of state transitions as well as the average time a dye is forced to reside in an off-state. Thus, the photobleaching of the sample is reduced, while resolution and recording speed are preserved. The power of the method is exemplified by imaging immunolabeled glial cells with up to 8-fold reduced photobleaching. | ||
| 700 | 1 | |a Engler, Andreas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rittweger, Eva |e VerfasserIn |4 aut | |
| 700 | 1 | |a Harke, Benjamin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Engelhardt, Johann |e VerfasserIn |0 (DE-588)1170115071 |0 (DE-627)1037102371 |0 (DE-576)512392536 |4 aut | |
| 700 | 1 | |a Hell, Stefan |d 1962- |e VerfasserIn |0 (DE-588)139815716 |0 (DE-627)613785681 |0 (DE-576)313195889 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Optics express |d Washington, DC : Optica, 1997 |g 19(2011), 6, Seite 5644-5657 |h Online-Ressource |w (DE-627)302718370 |w (DE-600)1491859-6 |w (DE-576)079876579 |x 1094-4087 |7 nnas |a Far-field optical nanoscopy with reduced number of state transition cycles |
| 773 | 1 | 8 | |g volume:19 |g year:2011 |g number:6 |g pages:5644-5657 |g extent:14 |a Far-field optical nanoscopy with reduced number of state transition cycles |
| 856 | 4 | 0 | |u https://doi.org/10.1364/OE.19.005644 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://opg.optica.org/oe/abstract.cfm?uri=oe-19-6-5644 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221021 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 139815716 |a Hell, Stefan |m 139815716:Hell, Stefan |d 130000 |e 130000PH139815716 |k 0/130000/ |p 6 |y j | ||
| 998 | |g 1170115071 |a Engelhardt, Johann |m 1170115071:Engelhardt, Johann |d 50000 |e 50000PE1170115071 |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN181961249X |e 4200559188 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["302718370"],"zdb":["1491859-6"],"issn":["1094-4087"]},"origin":[{"publisherPlace":"Washington, DC ; Washington, DC","dateIssuedDisp":"1997-","dateIssuedKey":"1997","publisher":"Optica ; Optical Society of America, OSA"}],"name":{"displayForm":["Optica"]},"part":{"extent":"14","volume":"19","text":"19(2011), 6, Seite 5644-5657","pages":"5644-5657","issue":"6","year":"2011"},"pubHistory":["1.1997 -"],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"Optica"},{"roleDisplay":"Herausgebendes Organ","display":"Optical Society of America","role":"isb"}],"language":["eng"],"recId":"302718370","note":["Gesehen am 10.01.2022","Herausgebendes Organ bis 2021: Optical Society of America"],"disp":"Far-field optical nanoscopy with reduced number of state transition cyclesOptics express","type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title_sort":"Optics express","title":"Optics express","subtitle":"the international electronic journal of optics"}]}],"physDesc":[{"extent":"14 S."}],"name":{"displayForm":["Thorsten Staudt, Andreas Engler, Eva Rittweger, Benjamin Harke, Johann Engelhardt, and Stefan W. Hell"]},"id":{"eki":["181961249X"],"doi":["10.1364/OE.19.005644"]},"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"11 Mar 2011"}],"recId":"181961249X","language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 21.10.2022"],"person":[{"roleDisplay":"VerfasserIn","display":"Staudt, Thorsten","role":"aut","family":"Staudt","given":"Thorsten"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Engler, Andreas","given":"Andreas","family":"Engler"},{"family":"Rittweger","given":"Eva","roleDisplay":"VerfasserIn","display":"Rittweger, Eva","role":"aut"},{"given":"Benjamin","family":"Harke","role":"aut","display":"Harke, Benjamin","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Engelhardt, Johann","role":"aut","family":"Engelhardt","given":"Johann"},{"role":"aut","display":"Hell, Stefan","roleDisplay":"VerfasserIn","given":"Stefan","family":"Hell"}],"title":[{"title":"Far-field optical nanoscopy with reduced number of state transition cycles","title_sort":"Far-field optical nanoscopy with reduced number of state transition cycles"}]} | ||
| SRT | |a STAUDTTHORFARFIELDOP1120 | ||