Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils: role of supercoiling
Amyloids are highly organized insoluble protein aggregates that are associated with a large variety of degenerative diseases. In this work, we investigated the anisotropic architecture of isolated human amyloid samples stained with Congo Red. This was performed by fluorescence detected linear dichro...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
|
| In: |
Journal of fluorescence
Year: 2011, Volume: 21, Issue: 3, Pages: 983-989 |
| ISSN: | 1573-4994 |
| DOI: | 10.1007/s10895-010-0684-3 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s10895-010-0684-3
|
| Author Notes: | Gábor Steinbach, István Pomozi, Dávid Péter Jánosa, Josef Makovitzky, Győző Garab |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1819615057 | ||
| 003 | DE-627 | ||
| 005 | 20230710110303.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221021s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10895-010-0684-3 |2 doi | |
| 035 | |a (DE-627)1819615057 | ||
| 035 | |a (DE-599)KXP1819615057 | ||
| 035 | |a (OCoLC)1389764837 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Steinbach, Gábor |e VerfasserIn |0 (DE-588)1270823248 |0 (DE-627)1819615510 |4 aut | |
| 245 | 1 | 0 | |a Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils |b role of supercoiling |c Gábor Steinbach, István Pomozi, Dávid Péter Jánosa, Josef Makovitzky, Győző Garab |
| 264 | 1 | |c 2011 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht: 17 June 2010 | ||
| 500 | |a Gesehen am 21.10.2022 | ||
| 520 | |a Amyloids are highly organized insoluble protein aggregates that are associated with a large variety of degenerative diseases. In this work, we investigated the anisotropic architecture of isolated human amyloid samples stained with Congo Red. This was performed by fluorescence detected linear dichroism (FDLD) imaging in a laser scanning confocal microscope that was equipped with a differential polarization attachment using high frequency modulation of the polarization state of the laser beam and a demodulation circuit. Two- and three-dimensional FDLD images of amyloids provided information on the orientation of the electric transition dipoles of the intercalated Congo Red molecules with unprecedented precision and spatial resolution. We show that, in accordance with linear dichroism imaging (Jin et al. Proc Natl Acad Sci USA 100:15294, 2003), amyloids exhibit strong anisotropy with preferential orientation of the dye molecules along the fibrils; estimations on the orientation angle, of around 45°, are given using a model calculation which takes into account the helical organization of the filaments and fibrils. Our data also show that FDLD images display large inhomogeneities, high local values with alternating signs and, in some regions, well identifiable µm-sized periodicities. These features of the anisotropic architecture are accounted for by supercoiling of helically organized amyloid fibrils. | ||
| 650 | 4 | |a Amyloid | |
| 650 | 4 | |a Anisotropy | |
| 650 | 4 | |a Fluorescence detected linear dichroism | |
| 650 | 4 | |a Laser scanning confocal microscopy | |
| 650 | 4 | |a Supercoiling | |
| 700 | 1 | |a Pomozi, István |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jánosa, Dávid Péter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Makovitzky, József |d 1942- |e VerfasserIn |0 (DE-588)1030495130 |0 (DE-627)735332622 |0 (DE-576)378344285 |4 aut | |
| 700 | 1 | |a Garab, Győző |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of fluorescence |d New York, NY : Springer Science + Business Media B.V., 1991 |g 21(2011), 3, Seite 983-989 |h Online-Ressource |w (DE-627)32057461X |w (DE-600)2016892-5 |w (DE-576)117648302 |x 1573-4994 |7 nnas |a Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils role of supercoiling |
| 773 | 1 | 8 | |g volume:21 |g year:2011 |g number:3 |g pages:983-989 |g extent:7 |a Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils role of supercoiling |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10895-010-0684-3 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221021 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1030495130 |a Makovitzky, József |m 1030495130:Makovitzky, József |p 4 | ||
| 999 | |a KXP-PPN1819615057 |e 420056467X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"7 S."}],"relHost":[{"pubHistory":["1.1991 -"],"part":{"pages":"983-989","issue":"3","year":"2011","extent":"7","text":"21(2011), 3, Seite 983-989","volume":"21"},"disp":"Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils role of supercoilingJournal of fluorescence","note":["Gesehen am 17.10.05"],"type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"recId":"32057461X","title":[{"title":"Journal of fluorescence","title_sort":"Journal of fluorescence"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedDisp":"1991-","publisher":"Springer Science + Business Media B.V. ; Kluwer","dateIssuedKey":"1991","publisherPlace":"New York, NY ; London"}],"id":{"issn":["1573-4994"],"eki":["32057461X"],"zdb":["2016892-5"]}}],"name":{"displayForm":["Gábor Steinbach, István Pomozi, Dávid Péter Jánosa, Josef Makovitzky, Győző Garab"]},"origin":[{"dateIssuedDisp":"2011","dateIssuedKey":"2011"}],"id":{"eki":["1819615057"],"doi":["10.1007/s10895-010-0684-3"]},"note":["Online veröffentlicht: 17 June 2010","Gesehen am 21.10.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1819615057","language":["eng"],"person":[{"roleDisplay":"VerfasserIn","display":"Steinbach, Gábor","role":"aut","family":"Steinbach","given":"Gábor"},{"role":"aut","display":"Pomozi, István","roleDisplay":"VerfasserIn","given":"István","family":"Pomozi"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Jánosa, Dávid Péter","given":"Dávid Péter","family":"Jánosa"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Makovitzky, József","given":"József","family":"Makovitzky"},{"family":"Garab","given":"Győző","display":"Garab, Győző","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils","subtitle":"role of supercoiling","title_sort":"Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils"}]} | ||
| SRT | |a STEINBACHGCONFOCALFL2011 | ||