Nucleosome mediated crosstalk between transcription factors at eukaryotic enhancers
A recent study of transcription regulation in Drosophila embryonic development revealed a complex non-monotonic dependence of gene expression on the distance between binding sites of repressor and activator proteins at the corresponding enhancer cis-regulatory modules (Fakhouri et al 2010 Mol. Syst....
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
10 June 2011
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| In: |
Physical biology
Year: 2011, Volume: 8, Issue: 4, Pages: 1-4 |
| ISSN: | 1478-3975 |
| DOI: | 10.1088/1478-3975/8/4/044001 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1088/1478-3975/8/4/044001 Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1088/1478-3975/8/4/044001 
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| Author Notes: | Vladimir B Teif and Karsten Rippe |
| Summary: | A recent study of transcription regulation in Drosophila embryonic development revealed a complex non-monotonic dependence of gene expression on the distance between binding sites of repressor and activator proteins at the corresponding enhancer cis-regulatory modules (Fakhouri et al 2010 Mol. Syst. Biol. 6 341). The repressor efficiency was high at small separations, low around 30 bp, reached a maximum at 50-60 bp, and decreased at larger distances to the activator binding sites. Here, we propose a straightforward explanation for the distance dependence of repressor activity by considering the effect of the presence of a nucleosome. Using a method that considers partial unwrapping of nucleosomal DNA from the histone octamer core, we calculated the dependence of activator binding on the repressor-activator distance and found a quantitative agreement with the distance dependence reported for the Drosophila enhancer element. In addition, the proposed model offers explanations for other distance-dependent effects at eukaryotic enhancers. |
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| Item Description: | Gesehen am 26.10.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1478-3975 |
| DOI: | 10.1088/1478-3975/8/4/044001 |