STED nanoscopy with mass-produced laser diodes

We show that far-field fluorescence nanoscopy by stimulated emission depletion (STED) can be realized with compact off-the-shelf laser diodes, such as those used in laser pointers and DVDs. A spatial resolution of 40-50 nm is attained by pulsing a 660 nm DVD-diode. The efficacy of these low-cost STE...

Full description

Saved in:
Bibliographic Details
Main Authors: Schrof, Susanne (Author) , Staudt, Thorsten M. (Author) , Rittweger, Eva (Author) , Wittenmayer, Nina (Author) , Dresbach, Thomas (Author) , Engelhardt, Johann (Author) , Hell, Stefan (Author)
Format: Article (Journal)
Language:English
Published: [2011]
In: Optics express
Year: 2011, Volume: 19, Issue: 9, Pages: 8066-8072
ISSN:1094-4087
DOI:10.1364/OE.19.008066
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1364/OE.19.008066
Verlag, lizenzpflichtig, Volltext: https://opg.optica.org/oe/abstract.cfm?uri=oe-19-9-8066
Get full text
Author Notes:Susanne Schrof, Thorsten Staudt, Eva Rittweger, Nina Wittenmayer, Thomas Dresbach, Johann Engelhardt, and Stefan W. Hell

MARC

LEADER 00000caa a2200000 c 4500
001 1820165701
003 DE-627
005 20230710104706.0
007 cr uuu---uuuuu
008 221027s2011 xx |||||o 00| ||eng c
024 7 |a 10.1364/OE.19.008066  |2 doi 
035 |a (DE-627)1820165701 
035 |a (DE-599)KXP1820165701 
035 |a (OCoLC)1389761372 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 29  |2 sdnb 
100 1 |a Schrof, Susanne  |e VerfasserIn  |0 (DE-588)1133575757  |0 (DE-627)889493715  |0 (DE-576)489508154  |4 aut 
245 1 0 |a STED nanoscopy with mass-produced laser diodes  |c Susanne Schrof, Thorsten Staudt, Eva Rittweger, Nina Wittenmayer, Thomas Dresbach, Johann Engelhardt, and Stefan W. Hell 
264 1 |c [2011] 
300 |b Illustrationen 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 27.10.2022 
520 |a We show that far-field fluorescence nanoscopy by stimulated emission depletion (STED) can be realized with compact off-the-shelf laser diodes, such as those used in laser pointers and DVDs. A spatial resolution of 40-50 nm is attained by pulsing a 660 nm DVD-diode. The efficacy of these low-cost STED microscopes in biological imaging is demonstrated by differentiating between clusters of the synaptic protein bassoon and transport vesicles in hippocampal neurons, based on the feature diameter. Our results facilitate the implementation of this all-molecular-transition based superresolution method in many applications ranging from nanoscale fluorescence imaging to nanoscale fluorescence sensing. 
700 1 |a Staudt, Thorsten M.  |e VerfasserIn  |0 (DE-588)139348115  |0 (DE-627)610248758  |0 (DE-576)311304966  |4 aut 
700 1 |a Rittweger, Eva  |e VerfasserIn  |0 (DE-588)14049247X  |0 (DE-627)703692968  |0 (DE-576)318504146  |4 aut 
700 1 |a Wittenmayer, Nina  |e VerfasserIn  |4 aut 
700 1 |a Dresbach, Thomas  |e VerfasserIn  |4 aut 
700 1 |a Engelhardt, Johann  |e VerfasserIn  |0 (DE-588)1170115071  |0 (DE-627)1037102371  |0 (DE-576)512392536  |4 aut 
700 1 |a Hell, Stefan  |d 1962-  |e VerfasserIn  |0 (DE-588)139815716  |0 (DE-627)613785681  |0 (DE-576)313195889  |4 aut 
773 0 8 |i Enthalten in  |t Optics express  |d Washington, DC : Optica, 1997  |g 19(2011), 9, Seite 8066-8072  |h Online-Ressource  |w (DE-627)302718370  |w (DE-600)1491859-6  |w (DE-576)079876579  |x 1094-4087  |7 nnas  |a STED nanoscopy with mass-produced laser diodes 
773 1 8 |g volume:19  |g year:2011  |g number:9  |g pages:8066-8072  |g extent:7  |a STED nanoscopy with mass-produced laser diodes 
856 4 0 |u https://doi.org/10.1364/OE.19.008066  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://opg.optica.org/oe/abstract.cfm?uri=oe-19-9-8066  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20221027 
993 |a Article 
994 |a 2011 
998 |g 139815716  |a Hell, Stefan  |m 139815716:Hell, Stefan  |d 130000  |e 130000PH139815716  |k 0/130000/  |p 7  |y j 
998 |g 1170115071  |a Engelhardt, Johann  |m 1170115071:Engelhardt, Johann  |d 50000  |e 50000PE1170115071  |k 0/50000/  |p 6 
999 |a KXP-PPN1820165701  |e 4202722183 
BIB |a Y 
SER |a journal 
JSO |a {"relHost":[{"title":[{"title_sort":"Optics express","title":"Optics express","subtitle":"the international electronic journal of optics"}],"pubHistory":["1.1997 -"],"part":{"text":"19(2011), 9, Seite 8066-8072","volume":"19","extent":"7","year":"2011","pages":"8066-8072","issue":"9"},"disp":"STED nanoscopy with mass-produced laser diodesOptics express","note":["Gesehen am 10.01.2022","Herausgebendes Organ bis 2021: Optical Society of America"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"302718370","corporate":[{"role":"isb","display":"Optica","roleDisplay":"Herausgebendes Organ"},{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"Optical Society of America"}],"language":["eng"],"origin":[{"publisherPlace":"Washington, DC ; Washington, DC","dateIssuedDisp":"1997-","dateIssuedKey":"1997","publisher":"Optica ; Optical Society of America, OSA"}],"id":{"eki":["302718370"],"zdb":["1491859-6"],"issn":["1094-4087"]},"name":{"displayForm":["Optica"]},"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"7 S.","noteIll":"Illustrationen"}],"name":{"displayForm":["Susanne Schrof, Thorsten Staudt, Eva Rittweger, Nina Wittenmayer, Thomas Dresbach, Johann Engelhardt, and Stefan W. Hell"]},"id":{"eki":["1820165701"],"doi":["10.1364/OE.19.008066"]},"origin":[{"dateIssuedDisp":"[2011]","dateIssuedKey":"2011"}],"language":["eng"],"recId":"1820165701","note":["Gesehen am 27.10.2022"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"role":"aut","display":"Schrof, Susanne","roleDisplay":"VerfasserIn","given":"Susanne","family":"Schrof"},{"family":"Staudt","given":"Thorsten M.","display":"Staudt, Thorsten M.","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Rittweger, Eva","role":"aut","family":"Rittweger","given":"Eva"},{"family":"Wittenmayer","given":"Nina","display":"Wittenmayer, Nina","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Dresbach, Thomas","role":"aut","family":"Dresbach","given":"Thomas"},{"family":"Engelhardt","given":"Johann","display":"Engelhardt, Johann","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Hell, Stefan","roleDisplay":"VerfasserIn","given":"Stefan","family":"Hell"}],"title":[{"title_sort":"STED nanoscopy with mass-produced laser diodes","title":"STED nanoscopy with mass-produced laser diodes"}]} 
SRT |a SCHROFSUSASTEDNANOSC2011