Interfering with interferon-γ signalling in intestinal epithelial cells: selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein
The intestinal epithelial barrier represents an important component in the pathogenesis of inflammatory bowel diseases. Interferon (IFN)-γ, a T helper type 1 (Th1) cytokine, regulated by the interleukin (IL)-18/IL-18 binding protein (bp) system, modulates the integrity of this barrier. The aim of th...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
[1 January 2011]
|
| In: |
Clinical & experimental immunology
Year: 2011, Jahrgang: 163, Heft: 1, Pages: 65-76 |
| ISSN: | 1365-2249 |
| DOI: | 10.1111/j.1365-2249.2010.04250.x |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1365-2249.2010.04250.x
|
| Verfasserangaben: | D. Schuhmann, P. Godoy, C. Weiß, A. Gerloff, M.V. Singer, S. Dooley and U. Böcker |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1820172317 | ||
| 003 | DE-627 | ||
| 005 | 20230710104632.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221027s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/j.1365-2249.2010.04250.x |2 doi | |
| 035 | |a (DE-627)1820172317 | ||
| 035 | |a (DE-599)KXP1820172317 | ||
| 035 | |a (OCoLC)1389761737 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Schuhmann, Daniel Hermann |d 1980- |e VerfasserIn |0 (DE-588)140580360 |0 (DE-627)619812745 |0 (DE-576)318957892 |4 aut | |
| 245 | 1 | 0 | |a Interfering with interferon-γ signalling in intestinal epithelial cells |b selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein |c D. Schuhmann, P. Godoy, C. Weiß, A. Gerloff, M.V. Singer, S. Dooley and U. Böcker |
| 246 | 3 | 3 | |a Interfering with interferon-gamma signalling in intestinal epithelial cells |
| 264 | 1 | |c [1 January 2011] | |
| 300 | |b Illustrationen | ||
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published: 16 November 2010 | ||
| 500 | |a Gesehen am 27.10.2022 | ||
| 520 | |a The intestinal epithelial barrier represents an important component in the pathogenesis of inflammatory bowel diseases. Interferon (IFN)-γ, a T helper type 1 (Th1) cytokine, regulated by the interleukin (IL)-18/IL-18 binding protein (bp) system, modulates the integrity of this barrier. The aim of this work was to study functionally the consequences of IFN-γ on intestinal epithelial cells (IEC) and to interfere selectively with identified adverse IFN-γ effects. IEC lines were stimulated with IFN-γ. IL-18 and IL-18bp were assessed by enzyme-linked immunosorbent assay. Staining of phosphatidylserine, DNA laddering, lactate dehydrogenase (LDH) release, cleavage of poly-adenosine diphosphate-ribose-polymerase (PARP) and activation of caspase-3 were analysed to determine cell death. Inhibitors of tyrosine kinase, caspase-3 or p38 mitogen-activated kinase ((MAP) activity were used. Cytokines were measured in supernatants of colonic biopsies of healthy controls and inflammatory bowel disease (IBD) patients. In IEC lines, IFN-γ up-regulated IL-18bp selectively. Ex vivo, IFN-γ was present in supernatants from cultured biopsies and up-regulated with inflammation. Contrary to previous reports, IFN-γ alone induced apoptosis in IEC lines, as demonstrated by phosphatidylserin staining, DNA cleavage and LDH release. Further, activation of caspase-3, PARP cleavage and expression of pro-apoptotic Bad were induced. Partial inhibition of caspase-3 and of p38 but not JAK tyrosine kinase, preserved up-regulation of IL-18bp expression. Selective inhibition of IFN-γ mediated apoptosis, while preserving its beneficial consequences on the ratio of IL-18/IL-18bp, could contribute to the integrity of the mucosal barrier in intestinal inflammation. | ||
| 700 | 1 | |a Godoy, Patricio |e VerfasserIn |0 (DE-588)141226242 |0 (DE-627)70391751X |0 (DE-576)322836514 |4 aut | |
| 700 | 1 | |a Weiß, Christel |d 1958- |e VerfasserIn |0 (DE-588)123287707 |0 (DE-627)082463417 |0 (DE-576)168356104 |4 aut | |
| 700 | 1 | |a Gerloff, Andreas |d 1980- |e VerfasserIn |0 (DE-588)140557342 |0 (DE-627)703714813 |0 (DE-576)317983717 |4 aut | |
| 700 | 1 | |a Singer, Manfred V. |d 1945- |e VerfasserIn |0 (DE-588)121081958 |0 (DE-627)081074069 |0 (DE-576)169905330 |4 aut | |
| 700 | 1 | |a Dooley, Steven |d 1960- |e VerfasserIn |0 (DE-588)1028589190 |0 (DE-627)73128951X |0 (DE-576)376074116 |4 aut | |
| 700 | 1 | |a Böcker, Ulrich |d 1965- |e VerfasserIn |0 (DE-588)1059893029 |0 (DE-627)798968990 |0 (DE-576)416025889 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Clinical & experimental immunology |d Oxford : Wiley-Blackwell, 1966 |g 163(2011), 1, Seite 65-76 |h Online-Ressource |w (DE-627)320599809 |w (DE-600)2020024-9 |w (DE-576)091140900 |x 1365-2249 |7 nnas |a Interfering with interferon-γ signalling in intestinal epithelial cells selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein |
| 773 | 1 | 8 | |g volume:163 |g year:2011 |g number:1 |g pages:65-76 |g extent:12 |a Interfering with interferon-γ signalling in intestinal epithelial cells selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein |
| 856 | 4 | 0 | |u https://doi.org/10.1111/j.1365-2249.2010.04250.x |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221027 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1059893029 |a Böcker, Ulrich |m 1059893029:Böcker, Ulrich |d 60000 |d 61100 |e 60000PB1059893029 |e 61100PB1059893029 |k 0/60000/ |k 1/60000/61100/ |p 7 |y j | ||
| 998 | |g 1028589190 |a Dooley, Steven |m 1028589190:Dooley, Steven |d 60000 |d 61100 |e 60000PD1028589190 |e 61100PD1028589190 |k 0/60000/ |k 1/60000/61100/ |p 6 | ||
| 998 | |g 121081958 |a Singer, Manfred V. |m 121081958:Singer, Manfred V. |d 60000 |d 61100 |e 60000PS121081958 |e 61100PS121081958 |k 0/60000/ |k 1/60000/61100/ |p 5 | ||
| 998 | |g 140557342 |a Gerloff, Andreas |m 140557342:Gerloff, Andreas |d 60000 |d 61100 |e 60000PG140557342 |e 61100PG140557342 |k 0/60000/ |k 1/60000/61100/ |p 4 | ||
| 998 | |g 123287707 |a Weiß, Christel |m 123287707:Weiß, Christel |d 60000 |d 60200 |d 60250 |e 60000PW123287707 |e 60200PW123287707 |e 60250PW123287707 |k 0/60000/ |k 1/60000/60200/ |k 2/60000/60200/60250/ |p 3 | ||
| 998 | |g 140580360 |a Schuhmann, Daniel Hermann |m 140580360:Schuhmann, Daniel Hermann |d 60000 |d 62300 |d 60000 |d 61100 |e 60000PS140580360 |e 62300PS140580360 |e 60000PS140580360 |e 61100PS140580360 |k 0/60000/ |k 1/60000/62300/ |k 0/60000/ |k 1/60000/61100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1820172317 |e 4202739817 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"eki":["1820172317"],"doi":["10.1111/j.1365-2249.2010.04250.x"]},"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"[1 January 2011]"}],"titleAlt":[{"title":"Interfering with interferon-gamma signalling in intestinal epithelial cells"}],"recId":"1820172317","physDesc":[{"noteIll":"Illustrationen","extent":"12 S."}],"relHost":[{"id":{"issn":["1365-2249"],"zdb":["2020024-9"],"eki":["320599809"],"doi":["10.1111/(ISSN)1365-2249"]},"type":{"bibl":"periodical","media":"Online-Ressource"},"part":{"text":"163(2011), 1, Seite 65-76","issue":"1","pages":"65-76","extent":"12","year":"2011","volume":"163"},"pubHistory":["1.1966 -"],"titleAlt":[{"title":"an official journal of the British Society for Immunology"},{"title":"Clinical and experimental immunology"}],"recId":"320599809","origin":[{"dateIssuedDisp":"1966-","publisher":"Wiley-Blackwell ; Blackwell Science ; Blackwell","publisherPlace":"Oxford ; Oxford ; Oxford [u.a.]","dateIssuedKey":"1966"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Clinical & experimental immunology","subtitle":"the journal of translational immunology","title":"Clinical & experimental immunology"}],"name":{"displayForm":["British Society for Immunology"]},"disp":"Interfering with interferon-γ signalling in intestinal epithelial cells selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding proteinClinical & experimental immunology","note":["Gesehen am 28.03.22"]}],"title":[{"title_sort":"Interfering with interferon-γ signalling in intestinal epithelial cells","subtitle":"selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein","title":"Interfering with interferon-γ signalling in intestinal epithelial cells"}],"language":["eng"],"person":[{"role":"aut","display":"Schuhmann, Daniel Hermann","given":"Daniel Hermann","family":"Schuhmann"},{"given":"Patricio","family":"Godoy","role":"aut","display":"Godoy, Patricio"},{"family":"Weiß","given":"Christel","display":"Weiß, Christel","role":"aut"},{"display":"Gerloff, Andreas","role":"aut","family":"Gerloff","given":"Andreas"},{"role":"aut","display":"Singer, Manfred V.","given":"Manfred V.","family":"Singer"},{"display":"Dooley, Steven","role":"aut","family":"Dooley","given":"Steven"},{"given":"Ulrich","family":"Böcker","role":"aut","display":"Böcker, Ulrich"}],"name":{"displayForm":["D. Schuhmann, P. Godoy, C. Weiß, A. Gerloff, M.V. Singer, S. Dooley and U. Böcker"]},"note":["Published: 16 November 2010","Gesehen am 27.10.2022"]} | ||
| SRT | |a SCHUHMANNDINTERFERIN1201 | ||