Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities
Hox proteins have similar binding specificities in vitro, yet they control different morphologies in vivo. This paradox has been partially solved with the identification of Hox low-affinity binding sites. However, anterior Hox proteins are more promiscuous than posterior Hox proteins, raising the qu...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
26 August 2022
|
| In: |
Nature Communications
Year: 2022, Jahrgang: 13, Pages: 1-17 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-022-32408-8 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-022-32408-8 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-022-32408-8 
|
| Verfasserangaben: | Pedro B. Pinto, Katrin Domsch, Xuefan Gao, Michaela Wölk, Julie Carnesecchi & Ingrid Lohmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1820223388 | ||
| 003 | DE-627 | ||
| 005 | 20240110133740.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221027s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-022-32408-8 |2 doi | |
| 035 | |a (DE-627)1820223388 | ||
| 035 | |a (DE-599)KXP1820223388 | ||
| 035 | |a (OCoLC)1361693696 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Pinto, Pedro |e VerfasserIn |0 (DE-588)1182242197 |0 (DE-627)1662578725 |4 aut | |
| 245 | 1 | 0 | |a Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities |c Pedro B. Pinto, Katrin Domsch, Xuefan Gao, Michaela Wölk, Julie Carnesecchi & Ingrid Lohmann |
| 264 | 1 | |c 26 August 2022 | |
| 300 | |b Illustrationen | ||
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.10.2022 | ||
| 520 | |a Hox proteins have similar binding specificities in vitro, yet they control different morphologies in vivo. This paradox has been partially solved with the identification of Hox low-affinity binding sites. However, anterior Hox proteins are more promiscuous than posterior Hox proteins, raising the question how anterior Hox proteins achieve specificity. We use the AP2x enhancer, which is activated in the maxillary head segment by the Hox TF Deformed (Dfd). This enhancer lacks canonical Dfd-Exd sites but contains several predicted low-affinity sites. Unexpectedly, these sites are strongly bound by Dfd-Exd complexes and their conversion into optimal Dfd-Exd sites results only in a modest increase in binding strength. These small variations in affinity change the sensitivity of the enhancer to different Dfd levels, resulting in perturbed AP-2 expression and maxillary morphogenesis. Thus, Hox-regulated morphogenesis seems to result from the co-evolution of Hox binding affinity and Hox dosage for precise target gene regulation. | ||
| 650 | 4 | |a Morphogenesis | |
| 650 | 4 | |a Pattern formation | |
| 650 | 4 | |a Transcriptional regulatory elements | |
| 700 | 1 | |a Domsch, Katrin |e VerfasserIn |0 (DE-588)1135800960 |0 (DE-627)890881103 |0 (DE-576)490137989 |4 aut | |
| 700 | 1 | |a Gao, Xuefan |d 1983- |e VerfasserIn |0 (DE-588)1074719115 |0 (DE-627)832381918 |0 (DE-576)442799942 |4 aut | |
| 700 | 1 | |a Wölk, Michaela |e VerfasserIn |4 aut | |
| 700 | 1 | |a Carnesecchi, Julie |e VerfasserIn |0 (DE-588)1156614937 |0 (DE-627)1019406356 |0 (DE-576)502288450 |4 aut | |
| 700 | 1 | |a Lohmann, Ingrid |e VerfasserIn |0 (DE-588)1047761831 |0 (DE-627)779177215 |0 (DE-576)401571688 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 13(2022), Artikel-ID 5037, Seite 1-17 |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities |
| 773 | 1 | 8 | |g volume:13 |g year:2022 |g elocationid:5037 |g pages:1-17 |g extent:17 |a Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-022-32408-8 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-022-32408-8 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221027 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 1047761831 |a Lohmann, Ingrid |m 1047761831:Lohmann, Ingrid |d 700000 |d 721000 |e 700000PL1047761831 |e 721000PL1047761831 |k 0/700000/ |k 1/700000/721000/ |p 6 |y j | ||
| 998 | |g 1156614937 |a Carnesecchi, Julie |m 1156614937:Carnesecchi, Julie |p 5 | ||
| 998 | |g 1074719115 |a Gao, Xuefan |m 1074719115:Gao, Xuefan |d 700000 |d 721000 |e 700000PG1074719115 |e 721000PG1074719115 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 998 | |g 1135800960 |a Domsch, Katrin |m 1135800960:Domsch, Katrin |d 700000 |d 721000 |e 700000PD1135800960 |e 721000PD1135800960 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 1182242197 |a Pinto, Pedro |m 1182242197:Pinto, Pedro |p 1 |x j | ||
| 999 | |a KXP-PPN1820223388 |e 4202862503 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1820223388","id":{"eki":["1820223388"],"doi":["10.1038/s41467-022-32408-8"]},"note":["Gesehen am 27.10.2022"],"physDesc":[{"extent":"17 S.","noteIll":"Illustrationen"}],"person":[{"roleDisplay":"VerfasserIn","role":"aut","display":"Pinto, Pedro","given":"Pedro","family":"Pinto"},{"display":"Domsch, Katrin","role":"aut","roleDisplay":"VerfasserIn","given":"Katrin","family":"Domsch"},{"given":"Xuefan","family":"Gao","roleDisplay":"VerfasserIn","role":"aut","display":"Gao, Xuefan"},{"family":"Wölk","given":"Michaela","roleDisplay":"VerfasserIn","display":"Wölk, Michaela","role":"aut"},{"role":"aut","display":"Carnesecchi, Julie","roleDisplay":"VerfasserIn","given":"Julie","family":"Carnesecchi"},{"family":"Lohmann","given":"Ingrid","roleDisplay":"VerfasserIn","role":"aut","display":"Lohmann, Ingrid"}],"name":{"displayForm":["Pedro B. Pinto, Katrin Domsch, Xuefan Gao, Michaela Wölk, Julie Carnesecchi & Ingrid Lohmann"]},"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"publisherPlace":"[London] ; [London]","dateIssuedDisp":"[2010]-","publisher":"Springer Nature ; Nature Publishing Group UK"}],"title":[{"title":"Nature Communications","title_sort":"Nature Communications"}],"language":["eng"],"disp":"Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinitiesNature Communications","part":{"volume":"13","extent":"17","pages":"1-17","text":"13(2022), Artikel-ID 5037, Seite 1-17","year":"2022"},"note":["Gesehen am 13.06.24"],"pubHistory":["2010-"],"physDesc":[{"extent":"Online-Ressource"}],"recId":"626457688","id":{"eki":["626457688"],"zdb":["2553671-0"],"issn":["2041-1723"]}}],"origin":[{"dateIssuedKey":"2022","dateIssuedDisp":"26 August 2022"}],"title":[{"title_sort":"Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities","title":"Specificity of the Hox member Deformed is determined by transcription factor levels and binding site affinities"}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a PINTOPEDROSPECIFICIT2620 | ||