Preanalytical stability of HIV-1 and HCV RNA: impact of storage and plasma separation from cells on blood donation testing by NAT
Background: The aim of this study was to evaluate the optimal preanalytical conditions prior to nucleic acid amplification technology (NAT) for human immunodeficiency virus-1 (HIV-1) or Hepatitis C virus (HCV) RNA in pools of 96 plasma specimens with regard to storage temperature, time and plasma se...
Gespeichert in:
| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
[April 2011]
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| In: |
Transfusion medicine
Year: 2011, Jahrgang: 21, Heft: 2, Pages: 99-106 |
| ISSN: | 1365-3148 |
| DOI: | 10.1111/j.1365-3148.2010.01051.x |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1365-3148.2010.01051.x Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-3148.2010.01051.x 
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| Verfasserangaben: | T.J. Schulze, C. Weiß, J. Luhm, C. Brockmann, S. Görg, & H. Hennig |
MARC
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| 245 | 1 | 0 | |a Preanalytical stability of HIV-1 and HCV RNA |b impact of storage and plasma separation from cells on blood donation testing by NAT |c T.J. Schulze, C. Weiß, J. Luhm, C. Brockmann, S. Görg, & H. Hennig |
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| 520 | |a Background: The aim of this study was to evaluate the optimal preanalytical conditions prior to nucleic acid amplification technology (NAT) for human immunodeficiency virus-1 (HIV-1) or Hepatitis C virus (HCV) RNA in pools of 96 plasma specimens with regard to storage temperature, time and plasma separation in a blood donation environment. Study design and methods: Changes in viral nucleic acid concentration of HIV-1 and HCV were observed for 5 days according to the Paul-Ehrlich-Institute's (PEI) guidelines that demand 95%-detection limit of at least 10 000 IU mL−1 for HIV-1 RNA and 5000 IU mL−1 for HCV RNA within a single donor blood specimen. Ninety-five per cent detection limits of HIV-1 RNA over 3 days after storage at either 5 or 21 °C were evaluated by using standardised HIV-1 RNA-positive plasma. Results: HCV RNA in whole blood samples proved to be more stable than HIV-1 RNA. Whole blood storage at 21 °C was shown to decrease the detectability of HIV-1 RNA even after only 18 h. Plasma samples once used for NAT at time 18 h did not alter viral stability up to 48 h after donation. Ninety-five per cent detection limits of HIV-1 RNA were securely below 10 000 IU mL−1 for 24 h after whole blood storage at 5 °C. Conclusions: These results may lead to a discussion around the most suitable preanalytical conditions in blood donation environments. Contrary to the current PEI guidelines that allow storage of whole blood specimens up to 18 h at 21 °C, these results suggest that immediate storage in a 5 °C container after blood donation is more suitable and would permit storage of whole blood up to 24 h prior to the separation of plasma from cells. | ||
| 650 | 4 | |a HCV | |
| 650 | 4 | |a HIV-1 | |
| 650 | 4 | |a preanalytical stability of HIV-1 and HCV RNA | |
| 650 | 4 | |a RNA viruses | |
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| 700 | 1 | |a Görg, S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hennig, H. |e VerfasserIn |4 aut | |
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