Cover Image

Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages

Inflammation and hypoxia impair alveolar barrier tightness, inhibit Na- and fluid reabsorption, and cause edema. We tested whether stimulated alveolar macrophages affect alveolar Na-transport and whether hypoxia aggravates the effects of inflammation, and tested for involved signaling pathways. Prim...

Full description

Saved in:
Bibliographic Details
Main Authors: Baloğlu, Emel (Author) , Velineni, Kalpana (Author) , Ermis-Kaya, Ezgi (Author) , Mairbäurl, Heimo (Author)
Format: Article (Journal)
Language:English
Published: 27 July 2022
In: International journal of molecular sciences
Year: 2022, Volume: 23, Issue: 15, Pages: 1-16
ISSN:1422-0067
DOI:10.3390/ijms23158315
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/ijms23158315
Verlag, kostenfrei, Volltext: https://www.mdpi.com/1422-0067/23/15/8315
Get full text
Author Notes:Emel Baloglu, Kalpana Velineni, Ezgi Ermis-Kaya, Heimo Mairbäurl

MARC

LEADER 00000caa a2200000 c 4500
001 1820683451
003 DE-627
005 20230118133936.0
007 cr uuu---uuuuu
008 221103s2022 xx |||||o 00| ||eng c
024 7 |a 10.3390/ijms23158315  |2 doi 
035 |a (DE-627)1820683451 
035 |a (DE-599)KXP1820683451 
035 |a (OCoLC)1361693295 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Baloğlu, Emel  |e VerfasserIn  |0 (DE-588)1122318499  |0 (DE-627)875645194  |0 (DE-576)481248757  |4 aut 
245 1 0 |a Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages  |c Emel Baloglu, Kalpana Velineni, Ezgi Ermis-Kaya, Heimo Mairbäurl 
264 1 |c 27 July 2022 
300 |a 16 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 03.11.2022 
520 |a Inflammation and hypoxia impair alveolar barrier tightness, inhibit Na- and fluid reabsorption, and cause edema. We tested whether stimulated alveolar macrophages affect alveolar Na-transport and whether hypoxia aggravates the effects of inflammation, and tested for involved signaling pathways. Primary rat alveolar type II cells (rA2) were co-cultured with rat alveolar macrophages (NR8383) or treated with NR8383-conditioned media after stimulation with lipopolysaccharide (LPS; 1 µg/mL) and exposed to normoxia and hypoxia (1.5% O2). LPS caused a fast, transient increase in TNFα and IL-6 mRNA in macrophages and a sustained increase in inducible nitric oxide synthase (NOS2) mRNA in macrophages and in rA2 cells resulting in elevated nitrite levels and secretion of TNF-α and IL-6 into culture media. In normoxia, 24 h of LPS treated NR8383 decreased the transepithelial electrical resistance (TEER) of co-cultures, of amiloride-sensitive short circuit current (ISCΔamil); whereas Na/K-ATPase activity was not affected. Inhibition was also seen with conditioned media from LPS-stimulated NR8383 on rA2, but was less pronounced after dialysis to remove small molecules and nitrite. The effect of LPS-stimulated macrophages on TEER and Na-transport was fully prevented by the iNOS-inhibitor L-NMMA applied to co-cultures and to rA2 mono-cultures. Hypoxia in combination with LPS-stimulated NR8383 totally abolished TEER and ISCΔamil. These results indicate that the LPS-stimulation of alveolar macrophages impairs alveolar epithelial Na-transport by NO-dependent mechanisms, where part of the NO is produced by rA2 induced by signals from LPS stimulated alveolar macrophages. 
650 4 |a alveolar epithelial Na-channels 
650 4 |a alveolar macrophages 
650 4 |a hypoxia 
650 4 |a inflammation 
650 4 |a iNOS 
650 4 |a Na/K-ATPase 
650 4 |a pulmonary edema 
700 1 |a Velineni, Kalpana  |e VerfasserIn  |4 aut 
700 1 |a Ermis-Kaya, Ezgi  |e VerfasserIn  |0 (DE-588)127192742X  |0 (DE-627)1820703118  |4 aut 
700 1 |a Mairbäurl, Heimo  |e VerfasserIn  |0 (DE-588)107812146X  |0 (DE-627)838003184  |0 (DE-576)44895110X  |4 aut 
773 0 8 |i Enthalten in  |t International journal of molecular sciences  |d Basel : Molecular Diversity Preservation International, 2000  |g 23(2022), 15, Artikel-ID 8315, Seite 1-16  |h Online-Ressource  |w (DE-627)316340715  |w (DE-600)2019364-6  |w (DE-576)281194653  |x 1422-0067  |7 nnas  |a Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages 
773 1 8 |g volume:23  |g year:2022  |g number:15  |g elocationid:8315  |g pages:1-16  |g extent:16  |a Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages 
856 4 0 |u https://doi.org/10.3390/ijms23158315  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u https://www.mdpi.com/1422-0067/23/15/8315  |x Verlag  |z kostenfrei  |3 Volltext 
951 |a AR 
992 |a 20221103 
993 |a Article 
994 |a 2022 
998 |g 107812146X  |a Mairbäurl, Heimo  |m 107812146X:Mairbäurl, Heimo  |d 910000  |d 910100  |d 50000  |e 910000PM107812146X  |e 910100PM107812146X  |e 50000PM107812146X  |k 0/910000/  |k 1/910000/910100/  |k 0/50000/  |p 4  |y j 
998 |g 127192742X  |a Ermis-Kaya, Ezgi  |m 127192742X:Ermis-Kaya, Ezgi  |p 3 
998 |g 1122318499  |a Baloğlu, Emel  |m 1122318499:Baloğlu, Emel  |p 1  |x j 
999 |a KXP-PPN1820683451  |e 4206034192 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"given":"Emel","family":"Baloğlu","role":"aut","display":"Baloğlu, Emel","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Velineni, Kalpana","role":"aut","family":"Velineni","given":"Kalpana"},{"display":"Ermis-Kaya, Ezgi","roleDisplay":"VerfasserIn","role":"aut","family":"Ermis-Kaya","given":"Ezgi"},{"family":"Mairbäurl","given":"Heimo","roleDisplay":"VerfasserIn","display":"Mairbäurl, Heimo","role":"aut"}],"title":[{"title":"Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages","title_sort":"Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophages"}],"note":["Gesehen am 03.11.2022"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1820683451","name":{"displayForm":["Emel Baloglu, Kalpana Velineni, Ezgi Ermis-Kaya, Heimo Mairbäurl"]},"origin":[{"dateIssuedKey":"2022","dateIssuedDisp":"27 July 2022"}],"id":{"eki":["1820683451"],"doi":["10.3390/ijms23158315"]},"physDesc":[{"extent":"16 S."}],"relHost":[{"pubHistory":["1.2000 -"],"part":{"year":"2022","issue":"15","pages":"1-16","text":"23(2022), 15, Artikel-ID 8315, Seite 1-16","volume":"23","extent":"16"},"titleAlt":[{"title":"IJMS"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Hypoxia aggravates inhibition of alveolar epithelial Na-transport by lipopolysaccharide-stimulation of alveolar macrophagesInternational journal of molecular sciences","note":["Gesehen am 17.09.20"],"language":["eng"],"recId":"316340715","title":[{"title":"International journal of molecular sciences","title_sort":"International journal of molecular sciences"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedDisp":"2000-","dateIssuedKey":"2000","publisher":"Molecular Diversity Preservation International","publisherPlace":"Basel"}],"id":{"zdb":["2019364-6"],"eki":["316340715"],"issn":["1422-0067","1661-6596"]}}]} 
SRT |a BALOGLUEMEHYPOXIAAGG2720 

Similar Items