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Inducible gene expression in GFAP+ progenitor cells of the SGZ and the dorsal wall of the SVZ: a novel tool to manipulate and trace adult neurogenesis

In the adult mammalian brain, neurogenesis originates from astrocyte-like stem cells. We generated a transgenic mouse line in which the tetracycline dependent transactivator (tTA) is expressed under the control of the murine GFAP promoter. In this mouse line, inducible gene expression targets virtua...

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Main Authors: Weber, Tillmann (Author) , Baier, Vera (Author) , Pauly, Rahel (Author) , Sahay, Amar (Author) , Baur, Max (Author) , Herrmann, Elke (Author) , Ciccolini, Francesca (Author) , Hen, René (Author) , Kronenberg, Golo (Author) , Bartsch, Dusan (Author)
Format: Article (Journal)
Language:English
Published: 3 February 2011
In: Glia
Year: 2011, Volume: 59, Issue: 4, Pages: 615-626
ISSN:1098-1136
DOI:10.1002/glia.21129
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/glia.21129
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.21129
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Author Notes:Tillmann Weber, Vera Baier, Rahel Pauly, Amar Sahay, Max Baur, Elke Herrmann, Francesca Ciccolini, René Hen, Golo Kronenberg, and Dusan Bartsch
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Summary:In the adult mammalian brain, neurogenesis originates from astrocyte-like stem cells. We generated a transgenic mouse line in which the tetracycline dependent transactivator (tTA) is expressed under the control of the murine GFAP promoter. In this mouse line, inducible gene expression targets virtually all GFAP-expressing stem-like cells in the dentate gyrus and a subset of GFAP-expressing progenitors located primarily in the dorsal wall/dorsolateral corner of the subventricular zone. Outside the neurogenic zones, astrocytes are infrequently targeted. We introduce a panel of transgenic mice which allow both inducible expression of candidate genes under control of the murine GFAP promoter and, at the same time, lineage tracing of all cells descendant from the original GFAP-positive cell. This new mouse line represents a versatile tool for functional analysis of neurogenesis and lineage tracing. © 2011 Wiley-Liss, Inc.
Item Description:Gesehen am 11.11.2022
Physical Description:Online Resource
ISSN:1098-1136
DOI:10.1002/glia.21129

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