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F-spondin regulates neuronal survival through activation of disabled-1 in the chicken ciliary ganglion

The extracellular membrane-associated protein F-spondin has been implicated in cell-matrix and cell-cell adhesion and plays an important role in axonal pathfinding. We report here that F-spondin is expressed in non-neuronal cells in the embryonic chicken ciliary ganglion (CG) and robustly promotes s...

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Main Authors: Peterziel, Heike (Author) , Sackmann, T. (Author) , Strelau, Jens (Author) , Kuhn, P. H. (Author) , Lichtenthaler, S. F. (Author) , Marom, K. (Author) , Klar, A. (Author) , Unsicker, Klaus (Author)
Format: Article (Journal)
Language:English
Published: 2011
In: Molecular and cellular neuroscience
Year: 2011, Volume: 46, Issue: 2, Pages: 483-497
ISSN:1095-9327
DOI:10.1016/j.mcn.2010.12.001
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.mcn.2010.12.001
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Author Notes:H. Peterziel, T. Sackmann, J. Strelau, P.H. Kuhn, S.F. Lichtenthaler, K. Marom, A. Klar, K. Unsicker
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Summary:The extracellular membrane-associated protein F-spondin has been implicated in cell-matrix and cell-cell adhesion and plays an important role in axonal pathfinding. We report here that F-spondin is expressed in non-neuronal cells in the embryonic chicken ciliary ganglion (CG) and robustly promotes survival of cultured CG neurons. Using deletion constructs of F-spondin we found that the amino-terminal Reelin/Spondin domain cooperates with thrombospondin type 1 repeat (TSR) 6, a functional TGFβ-activation domain. In ovo treatment with blocking antibodies raised against the Reelin/Spondin domain or the TSR-domains caused increased apoptosis of CG neurons during the phase of programmed cell death and loss of about 30% of the neurons compared to controls. The Reelin/Spondin domain receptor - APP and its downstream signalling molecule disabled-1 are expressed in CG neurons. F-spondin induced rapid phosphorylation of disabled-1. Moreover, both blocking the central APP domain and interference with disabled-1 signalling disrupted the survival promoting effect of F-spondin. Taken together, our data suggest that F-spondin can promote neuron survival by a mechanism involving the Reelin/Spondin and the TSR domains.
Item Description:Available online: 9 December 2010
Gesehen am 29.11.2022
Physical Description:Online Resource
ISSN:1095-9327
DOI:10.1016/j.mcn.2010.12.001

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