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The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption

Fertilization triggers a rise in intracellular Ca2+ concentration ([Ca2+]i) in the egg that initiates a series of events known as egg activation. These events include cortical granule exocytosis that establishes a block to polyspermy, resumption of meiosis, and recruitment of maternal mRNAs into pol...

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Hauptverfasser: Backs, Johannes (VerfasserIn) , Stein, Paula (VerfasserIn) , Backs, Thea (VerfasserIn) , Duncan, Francesca E. (VerfasserIn) , Grueter, Chad E. (VerfasserIn) , McAnally, John (VerfasserIn) , Qi, Xiaoxia (VerfasserIn) , Schultz, Richard M. (VerfasserIn) , Olson, Eric N. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2010
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2010, Jahrgang: 107, Heft: 1, Pages: 81-86
ISSN:1091-6490
DOI:10.1073/pnas.0912658106
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.0912658106
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/doi/full/10.1073/pnas.0912658106
Volltext
Verfasserangaben:Johannes Backs, Paula Stein, Thea Backs, Francesca E. Duncan, Chad E. Grueter, John McAnally, Xiaoxia Qi, Richard M. Schultz, and Eric N. Olson
Beschreibung
Zusammenfassung:Fertilization triggers a rise in intracellular Ca2+ concentration ([Ca2+]i) in the egg that initiates a series of events known as egg activation. These events include cortical granule exocytosis that establishes a block to polyspermy, resumption of meiosis, and recruitment of maternal mRNAs into polysomes for translation. Several calcium-dependent proteins, including calcium/calmodulin-dependent protein kinase II (CaMKII), have been implicated in egg activation. However, the precise role of CaMKII in mediating specific events of egg activation and the identity of the isoform(s) present in mouse eggs have not been unequivocally established. Through targeted deletion of the γ isoform of CaMKII, we find that CaMKIIγ is the predominant CaMKII isoform in mouse eggs and that it is essential for egg activation. Although CaMKIIγ−/− eggs exhibit a normal pattern of Ca2+ oscillations after insemination and undergo cortical granule exocytosis, they fail to resume meiosis or to recruit maternal mRNAs. Surprisingly, we find that the recruitment of maternal mRNAs does not directly depend on CaMKII, but requires elevated [Ca2+]i and metaphase II exit. We conclude that CaMKIIγ specifically controls mouse egg activation by regulating cell cycle resumption.
Beschreibung:veröffentlicht Dezember 29, 2009
Gesehen am 29.11.2022
Beschreibung:Online Resource
ISSN:1091-6490
DOI:10.1073/pnas.0912658106

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