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Immunohistochemical analyses of paraffin-embedded sections after primary surgery or trimodality treatment in esophageal carcinoma

Background - The microscopic tumor extension before, during or after radiochemotherapy (RCHT) and its correlation with the tumor microenvironment (TME) are presently unknown. This information is, however, crucial in the era of image-guided, adaptive high-precision photon or particle therapy. - Mater...

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Main Authors: Igbo, Benjamin Terfa (Author) , Linge, Annett (Author) , Frosch, Susanne (Author) , Suckert, Theresa (Author) , Stolz-Kieslich, Liane (Author) , Löck, Steffen (Author) , Kumaravadivel, Mani Sankari (Author) , Welsch, Thilo (Author) , Weitz, Jürgen (Author) , Sommer, Ulrich (Author) , Aust, Daniela E. (Author) , Troost, Esther Gera Cornelia (Author)
Format: Article (Journal)
Language:English
Published: 3 August 2022
In: Clinical and translational radiation oncology
Year: 2022, Volume: 36, Pages: 106-112
ISSN:2405-6308
DOI:10.1016/j.ctro.2022.08.001
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ctro.2022.08.001
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2405630822000635
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Author Notes:Benjamin Terfa Igbo, Annett Linge, Susanne Frosch, Theresa Suckert, Liane Stolz-Kieslich, Steffen Löck, Mani Sankari Kumaravadivel, Thilo Welsch, Jürgen Weitz, Ulrich Sommer, Daniela Aust, Esther G.C. Troost
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Summary:Background - The microscopic tumor extension before, during or after radiochemotherapy (RCHT) and its correlation with the tumor microenvironment (TME) are presently unknown. This information is, however, crucial in the era of image-guided, adaptive high-precision photon or particle therapy. - Materials and methods - In this pilot study, we analyzed formalin-fixed paraffin-embedded (FFPE) tumor resection specimen from patients with histologically confirmed squamous cell carcinoma (SCC; n = 10) or adenocarcinoma (A; n = 10) of the esophagus, having undergone neoadjuvant radiochemotherapy followed by resection (NRCHT + R) or resection (R)]. FFPE tissue sections were analyzed by immunohistochemistry regarding tumor hypoxia (HIF-1α), proliferation (Ki67), immune status (PD1), cancer cell stemness (CXCR4), and p53 mutation status. Marker expression in HIF-1α subvolumes was part of a sub-analysis. Statistical analyses were performed using one-sided Mann-Whitney tests and Bland-Altman analysis. - Results - In both SCC and AC patients, the overall percentages of positive tumor cells among the five TME markers, namely HIF-1α, Ki67, p53, CXCR4 and PD1 after NRCHT were lower than in the R cohort. However, only PD1 in SCC and Ki67 in AC showed significant association (Ki67: p = 0.03, PD1: p = 0.02). In the sub-analysis of hypoxic subvolumes among the AC patients, the percentage of positive tumor cells within hypoxic regions were statistically significantly lower in the NRCHT than in the R cohort across all the markers except for PD1. - Conclusion - In this pilot study, we showed changes in the TME induced by NRCHT in both SCC and AC. These findings will be correlated with microscopic tumor extension measurements in a subsequent cohort of patients.
Item Description:Gesehen am 31.11.2022
Physical Description:Online Resource
ISSN:2405-6308
DOI:10.1016/j.ctro.2022.08.001

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