Development and validation of an immunoluminometric assay (ilma) for fetuin-a-glycoprotein
Background: To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed. Methods: For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester. Results: The lower de...
Gespeichert in:
| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
30 Dec 2009
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| In: |
Journal of immunoassay & immunochemistry
Year: 2009, Jahrgang: 31, Heft: 1, Pages: 10-23 |
| ISSN: | 1532-4230 |
| DOI: | 10.1080/15321810903404830 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1080/15321810903404830
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| Verfasserangaben: | Amelie Bäumer, Heinrich Schmidt-Gayk, Michael Aulmann, Ti-Sun Kim |
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| 245 | 1 | 0 | |a Development and validation of an immunoluminometric assay (ilma) for fetuin-a-glycoprotein |c Amelie Bäumer, Heinrich Schmidt-Gayk, Michael Aulmann, Ti-Sun Kim |
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| 520 | |a Background: To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed. Methods: For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester. Results: The lower detection threshold was 0.013 g/L, with the normal range 0.20-0.87 g/L (arithmetic mean 0.437 ± 0.118 g/L). Serum fetuin-A levels in the dialysis patients were significantly lower (arithmetic mean: 0.352 ± 0.099 g/L, p < 0.0001). Conclusion: This ILMA has been proved to be a reliable method for the determination of serum fetuin-A concentrations in dialysis patients. | ||
| 650 | 4 | |a cardiovascular diseases | |
| 650 | 4 | |a dialysis patients | |
| 650 | 4 | |a fetuin-A/α2-Heremans-Schmid-glycoprotein | |
| 650 | 4 | |a ILMA | |
| 650 | 4 | |a Vascular calcification | |
| 700 | 1 | |a Schmidt-Gayk, Heinrich |e VerfasserIn |4 aut | |
| 700 | 1 | |a Aulmann, Michael |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kim, Ti-Sun |e VerfasserIn |0 (DE-588)107028842X |0 (DE-627)82440596X |0 (DE-576)430000936 |4 aut | |
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