Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation
High risk human papillomaviruses are squamous epitheliotropic viruses that may cause cervical and other cancers. HPV replication depends on squamous epithelial differentiation. Transformation of HPV-infected cells goes along with substantial alteration of the viral gene expression profile and prefer...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
September 7, 2011
|
| In: |
PLOS ONE
Year: 2011, Jahrgang: 6, Heft: 9, Pages: 1-13 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0024451 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0024451 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0024451 
|
| Verfasserangaben: | Svetlana Vinokurova, Magnus von Knebel Doeberitz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1826624392 | ||
| 003 | DE-627 | ||
| 005 | 20230710100235.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221208s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0024451 |2 doi | |
| 035 | |a (DE-627)1826624392 | ||
| 035 | |a (DE-599)KXP1826624392 | ||
| 035 | |a (OCoLC)1389741078 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Vinokurova, Svetlana |e VerfasserIn |0 (DE-588)141462949 |0 (DE-627)627798160 |0 (DE-576)323993605 |4 aut | |
| 245 | 1 | 0 | |a Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation |c Svetlana Vinokurova, Magnus von Knebel Doeberitz |
| 264 | 1 | |c September 7, 2011 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.12.2022 | ||
| 520 | |a High risk human papillomaviruses are squamous epitheliotropic viruses that may cause cervical and other cancers. HPV replication depends on squamous epithelial differentiation. Transformation of HPV-infected cells goes along with substantial alteration of the viral gene expression profile and preferentially occurs at transformation zones usually at the uterine cervix. Methylation of the viral genome may affect regulatory features that control transcription and replication of the viral genome. Therefore, we analyzed the methylation pattern of the HPV16 upstream regulatory region (URR) during squamous epithelial differentiation and neoplastic transformation and analyzed how shifts in the HPV URR methylome may affect viral gene expression and replication. HPV 16 positive biopsy sections encompassing all stages of an HPV infection (latent, permissive and transforming) were micro-dissected and DNA was isolated from cell fractions representing the basal, intermediate, and superficial cell layers, each, as well as from transformed p16INK4a-positive cells. We observed fundamental changes in the methylation profile of transcription factor binding sites in the HPV16 upstream regulatory region linked to the squamous epithelial differentiation stage. Squamous epithelial transformation indicated by p16INK4a overexpression was associated with methylation of the distal E2 binding site 1 leading to hyper-activation of the HPV 16 URR. Adjacent normal but HPV 16-infected epithelial areas retained hyper-methylated HPV DNA suggesting that these viral genomes were inactivated. These data suggest that distinct shifts of the HPV 16 methylome are linked to differentiation dependent transcription and replication control and may trigger neoplastic transformation. | ||
| 650 | 4 | |a Cell differentiation | |
| 650 | 4 | |a DNA isolation | |
| 650 | 4 | |a DNA methylation | |
| 650 | 4 | |a Genomics | |
| 650 | 4 | |a HPV-16 | |
| 650 | 4 | |a Human papillomavirus infection | |
| 650 | 4 | |a Polymerase chain reaction | |
| 650 | 4 | |a Viral genome | |
| 700 | 1 | |a Knebel Doeberitz, Magnus von |e VerfasserIn |0 (DE-588)1022165291 |0 (DE-627)71693289X |0 (DE-576)364934239 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 6(2011), 9 vom: Sept., Artikel-ID e24451, Seite 1-13 |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation |
| 773 | 1 | 8 | |g volume:6 |g year:2011 |g number:9 |g month:09 |g elocationid:e24451 |g pages:1-13 |g extent:13 |a Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pone.0024451 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0024451 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221208 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1022165291 |a Knebel Doeberitz, Magnus von |m 1022165291:Knebel Doeberitz, Magnus von |d 910000 |d 912000 |e 910000PK1022165291 |e 912000PK1022165291 |k 0/910000/ |k 1/910000/912000/ |p 2 |y j | ||
| 998 | |g 141462949 |a Vinokurova, Svetlana |m 141462949:Vinokurova, Svetlana |d 910000 |d 912000 |e 910000PV141462949 |e 912000PV141462949 |k 0/910000/ |k 1/910000/912000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1826624392 |e 4228515282 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"role":"aut","display":"Vinokurova, Svetlana","roleDisplay":"VerfasserIn","given":"Svetlana","family":"Vinokurova"},{"role":"aut","display":"Knebel Doeberitz, Magnus von","roleDisplay":"VerfasserIn","given":"Magnus von","family":"Knebel Doeberitz"}],"title":[{"title":"Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation","title_sort":"Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation"}],"recId":"1826624392","language":["eng"],"note":["Gesehen am 08.12.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Svetlana Vinokurova, Magnus von Knebel Doeberitz"]},"id":{"eki":["1826624392"],"doi":["10.1371/journal.pone.0024451"]},"origin":[{"dateIssuedDisp":"September 7, 2011","dateIssuedKey":"2011"}],"relHost":[{"name":{"displayForm":["Public Library of Science"]},"id":{"issn":["1932-6203"],"eki":["523574592"],"zdb":["2267670-3"]},"origin":[{"publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisher":"PLOS ; PLoS"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}],"language":["eng"],"corporate":[{"role":"isb","display":"Public Library of Science","roleDisplay":"Herausgebendes Organ"}],"recId":"523574592","note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"disp":"Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformationPLOS ONE","type":{"bibl":"periodical","media":"Online-Ressource"},"part":{"volume":"6","text":"6(2011), 9 vom: Sept., Artikel-ID e24451, Seite 1-13","extent":"13","year":"2011","issue":"9","pages":"1-13"},"pubHistory":["1.2006 -"]}],"physDesc":[{"extent":"13 S."}]} | ||
| SRT | |a VINOKUROVADIFFERENTI7201 | ||