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Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including variant analysis by mass spectrometry in placental tissue

Among neonates, tested positive for SARS-CoV-2, the majority of infections occur through postpartum transmission. Only few reports describe intrauterine or intrapartum SARS-CoV-2 infections in newborns. To understand the route of transmission, detection of the virus or virus nucleic acid in the plac...

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Main Authors: Wierz, Marina (Author) , Sauerbrei, Beate (Author) , Wandernoth, Petra Melitta (Author) , Kriegsmann, Mark (Author) , Casadonte, Rita (Author) , Kriegsmann, Katharina (Author) , Kriegsmann, Jörg (Author)
Format: Article (Journal)
Language:English
Published: 14 March 2022
In: Viruses
Year: 2022, Volume: 14, Issue: 3, Pages: 1-11
ISSN:1999-4915
DOI:10.3390/v14030604
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/v14030604
Verlag, kostenfrei, Volltext: https://www.mdpi.com/1999-4915/14/3/604
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Author Notes:Marina Wierz, Beate Sauerbrei, Petra Wandernoth, Mark Kriegsmann, Rita Casadonte, Katharina Kriegsmann and Jörg Kriegsmann

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520 |a Among neonates, tested positive for SARS-CoV-2, the majority of infections occur through postpartum transmission. Only few reports describe intrauterine or intrapartum SARS-CoV-2 infections in newborns. To understand the route of transmission, detection of the virus or virus nucleic acid in the placenta and amniotic tissue are of special interest. Current methods to detect SARS-CoV-2 in placental tissue are immunohistochemistry, electron microscopy, in-situ hybridization, polymerase chain reaction (PCR) and next-generation sequencing. Recently, we described an alternative method for the detection of viral ribonucleic acid (RNA), by combination of reverse transcriptase-PCR and mass spectrometry (MS) in oropharyngeal and oral swabs. In this report, we could detect SARS-CoV-2 in formal-fixed and paraffin-embedded (FFPE) placental and amniotic tissue by multiplex RT-PCR MS. Additionally, we could identify the British variant (B.1.1.7) of the virus in this tissue by the same methodology. Combination of RT-PCR with MS is a fast and easy method to detect SARS-CoV-2 viral RNA, including specific variants in FFPE tissue. 
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