Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes
Glutamine synthetase (GS) is a key enzyme that metabolizes glutamate into glutamine. While GS is highly enriched in astrocytes, expression in other glial lineages has been noted. Using a combination of reporter mice and cell type-specific markers, we show that GS is expressed in myelinating oligoden...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 August 2021
|
| In: |
Glia
Year: 2021, Jahrgang: 69, Heft: 12, Pages: 2812-2827 |
| ISSN: | 1098-1136 |
| DOI: | 10.1002/glia.24071 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/glia.24071 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24071 
|
| Verfasserangaben: | Lucile Ben Haim, Lucas Schirmer, Amel Zulji, Khalida Sabeur, Brice Tiret, Matthieu Ribon, Sandra Chang, Wouter H. Lamers, Séverine Boillée, Myriam M. Chaumeil, David H. Rowitch |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1826872604 | ||
| 003 | DE-627 | ||
| 005 | 20230428033638.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221213s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/glia.24071 |2 doi | |
| 035 | |a (DE-627)1826872604 | ||
| 035 | |a (DE-599)KXP1826872604 | ||
| 035 | |a (OCoLC)1361713786 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Ben Haim, Lucile |e VerfasserIn |0 (DE-588)1275432468 |0 (DE-627)1826870423 |4 aut | |
| 245 | 1 | 0 | |a Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes |c Lucile Ben Haim, Lucas Schirmer, Amel Zulji, Khalida Sabeur, Brice Tiret, Matthieu Ribon, Sandra Chang, Wouter H. Lamers, Séverine Boillée, Myriam M. Chaumeil, David H. Rowitch |
| 264 | 1 | |c 15 August 2021 | |
| 300 | |a 16 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a First Published: 15 August 2021 | ||
| 500 | |a Gesehen am 13.12.2022 | ||
| 520 | |a Glutamine synthetase (GS) is a key enzyme that metabolizes glutamate into glutamine. While GS is highly enriched in astrocytes, expression in other glial lineages has been noted. Using a combination of reporter mice and cell type-specific markers, we show that GS is expressed in myelinating oligodendrocytes (OL) but not oligodendrocyte progenitor cells of the mouse and human ventral spinal cord. To investigate the role of GS in mature OL, we used a conditional knockout (cKO) approach to selectively delete GS-encoding gene (Glul) in OL, which caused a significant decrease in glutamine levels on mouse spinal cord extracts. GS cKO mice (CNP-cre+:Glulfl/fl) showed no differences in motor neuron numbers, size or axon density; OL differentiation and myelination in the ventral spinal cord was normal up to 6 months of age. Interestingly, GS cKO mice showed a transient and specific decrease in peak force while locomotion and motor coordination remained unaffected. Last, GS expression in OL was increased in chronic pathological conditions in both mouse and humans. We found a disease-stage dependent increase of OL expressing GS in the ventral spinal cord of SOD1(G93A) mouse model of amyotrophic lateral sclerosis. Moreover, we showed that GLUL transcripts levels were increased in OL in leukocortical tissue from multiple sclerosis but not control patients. These findings provide evidence towards OL-encoded GS function in spinal cord sensorimotor axis, which is dysregulated in chronic neurological diseases. | ||
| 650 | 4 | |a amyotrophic lateral sclerosis | |
| 650 | 4 | |a glutamine synthetase | |
| 650 | 4 | |a motor neurons | |
| 650 | 4 | |a oligodendrocytes | |
| 650 | 4 | |a peak strength | |
| 650 | 4 | |a spinal cord | |
| 700 | 1 | |a Schirmer, Lucas |d 1981- |e VerfasserIn |0 (DE-588)140880631 |0 (DE-627)70381737X |0 (DE-576)321212088 |4 aut | |
| 700 | 1 | |a Zulji, Amel |d 1991- |e VerfasserIn |0 (DE-588)1275433561 |0 (DE-627)182687187X |4 aut | |
| 700 | 1 | |a Sabeur, Khalida |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tiret, Brice |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ribon, Matthieu |e VerfasserIn |4 aut | |
| 700 | 1 | |a Chang, Sandra |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lamers, Wouter H. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Boillée, Séverine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Chaumeil, Myriam M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rowitch, David H. |e VerfasserIn |0 (DE-588)1201055571 |0 (DE-627)1684201721 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Glia |d Bognor Regis [u.a.] : Wiley-Liss, 1988 |g 69(2021), 12 vom: Dez., Seite 2812-2827 |h Online-Ressource |w (DE-627)269532846 |w (DE-600)1474828-9 |w (DE-576)090854446 |x 1098-1136 |7 nnas |a Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes |
| 773 | 1 | 8 | |g volume:69 |g year:2021 |g number:12 |g month:12 |g pages:2812-2827 |g extent:16 |a Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes |
| 856 | 4 | 0 | |u https://doi.org/10.1002/glia.24071 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24071 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20221213 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1275433561 |a Zulji, Amel |m 1275433561:Zulji, Amel |d 60000 |d 62700 |e 60000PZ1275433561 |e 62700PZ1275433561 |k 0/60000/ |k 1/60000/62700/ |p 3 | ||
| 998 | |g 140880631 |a Schirmer, Lucas |m 140880631:Schirmer, Lucas |d 60000 |d 62700 |e 60000PS140880631 |e 62700PS140880631 |k 0/60000/ |k 1/60000/62700/ |p 2 | ||
| 999 | |a KXP-PPN1826872604 |e 4229442297 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"16 S."}],"recId":"1826872604","origin":[{"dateIssuedDisp":"15 August 2021","dateIssuedKey":"2021"}],"person":[{"display":"Ben Haim, Lucile","family":"Ben Haim","given":"Lucile","role":"aut"},{"display":"Schirmer, Lucas","family":"Schirmer","role":"aut","given":"Lucas"},{"family":"Zulji","given":"Amel","role":"aut","display":"Zulji, Amel"},{"role":"aut","given":"Khalida","family":"Sabeur","display":"Sabeur, Khalida"},{"display":"Tiret, Brice","family":"Tiret","role":"aut","given":"Brice"},{"family":"Ribon","given":"Matthieu","role":"aut","display":"Ribon, Matthieu"},{"display":"Chang, Sandra","family":"Chang","role":"aut","given":"Sandra"},{"role":"aut","given":"Wouter H.","family":"Lamers","display":"Lamers, Wouter H."},{"display":"Boillée, Séverine","given":"Séverine","role":"aut","family":"Boillée"},{"display":"Chaumeil, Myriam M.","role":"aut","given":"Myriam M.","family":"Chaumeil"},{"display":"Rowitch, David H.","given":"David H.","role":"aut","family":"Rowitch"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"recId":"269532846","disp":"Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytesGlia","origin":[{"dateIssuedKey":"1988","dateIssuedDisp":"1988-","publisherPlace":"Bognor Regis [u.a.]","publisher":"Wiley-Liss"}],"pubHistory":["1.1988 -"],"language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 04.03.08"],"id":{"doi":["10.1002/(ISSN)1098-1136"],"zdb":["1474828-9"],"issn":["1098-1136"],"eki":["269532846"]},"part":{"pages":"2812-2827","text":"69(2021), 12 vom: Dez., Seite 2812-2827","extent":"16","issue":"12","year":"2021","volume":"69"},"title":[{"title":"Glia","title_sort":"Glia"}]}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"note":["First Published: 15 August 2021","Gesehen am 13.12.2022"],"title":[{"title":"Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes","title_sort":"Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes"}],"name":{"displayForm":["Lucile Ben Haim, Lucas Schirmer, Amel Zulji, Khalida Sabeur, Brice Tiret, Matthieu Ribon, Sandra Chang, Wouter H. Lamers, Séverine Boillée, Myriam M. Chaumeil, David H. Rowitch"]},"id":{"eki":["1826872604"],"doi":["10.1002/glia.24071"]}} | ||
| SRT | |a BENHAIMLUCEVIDENCEFO1520 | ||