Buchumschlag

First experiences with the Spectrum Compact CE System

The newly released Spectrum Compact CE System by Promega is a capillary electrophoresis instrument developed for DNA-fragment separation and sequencing. In this study, its compatibility to 8 commercial short tandem repeat (STR) kits from 4 different manufacturers, reproducibility (sizing precision,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Burgardt, Nastasja (VerfasserIn) , Weissenberger, Melanie (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2022
In: International journal of legal medicine
Year: 2022, Jahrgang: 136, Heft: 1, Pages: 55-72
ISSN:1437-1596
DOI:10.1007/s00414-021-02673-1
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1007/s00414-021-02673-1
Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1007/s00414-021-02673-1
Volltext
Verfasserangaben:Nastasja Burgardt, Melanie Weissenberger

MARC

LEADER 00000caa a2200000 c 4500
001 1827025557
003 DE-627
005 20230428033648.0
007 cr uuu---uuuuu
008 221214s2022 xx |||||o 00| ||eng c
024 7 |a 10.1007/s00414-021-02673-1  |2 doi 
035 |a (DE-627)1827025557 
035 |a (DE-599)KXP1827025557 
035 |a (OCoLC)1360436629 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Burgardt, Nastasja  |e VerfasserIn  |0 (DE-588)1275511740  |0 (DE-627)1827026065  |4 aut 
245 1 0 |a First experiences with the Spectrum Compact CE System  |c Nastasja Burgardt, Melanie Weissenberger 
264 1 |c 2022 
300 |a 17 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Online veröffentlicht am 19. Oktober 2021 
500 |a Gesehen am 14.12.2022 
520 |a The newly released Spectrum Compact CE System by Promega is a capillary electrophoresis instrument developed for DNA-fragment separation and sequencing. In this study, its compatibility to 8 commercial short tandem repeat (STR) kits from 4 different manufacturers, reproducibility (sizing precision, accuracy and concordance) and robustness (sensitivity and mixture resolution) were tested and compared to the ABI PRISM® 310 Genetic Analyzer. The instrument was able to successfully analyse amplicons of all tested kits, proved to be as precise as claimed by manufacturer specifications and was shown to be more robust than the ABI PRISM® 310 Genetic Analyzer in some aspects. Analyses on the Spectrum Compact CE System were able to resolve peaks with length differences of 1-basepair in the short and long fragment range and mixtures of mixture ratios down to 1:30. We describe the advantages and limitations we have observed so far working with this instrument in our forensic genetics laboratory. 
650 4 |a DNA 
650 4 |a Forensic genetics 
650 4 |a Promega 
650 4 |a Spectrum Compact CE System 
650 4 |a STR 
700 1 |a Weissenberger, Melanie  |d 1982-  |e VerfasserIn  |0 (DE-588)141981334  |0 (DE-627)632855576  |0 (DE-576)327157747  |4 aut 
773 0 8 |i Enthalten in  |t International journal of legal medicine  |d Berlin : Springer, 1990  |g 136(2022), 1, Seite 55-72  |h Online-Ressource  |w (DE-627)253724295  |w (DE-600)1459222-8  |w (DE-576)072373148  |x 1437-1596  |7 nnas  |a First experiences with the Spectrum Compact CE System 
773 1 8 |g volume:136  |g year:2022  |g number:1  |g pages:55-72  |g extent:17  |a First experiences with the Spectrum Compact CE System 
856 4 0 |u https://doi.org/10.1007/s00414-021-02673-1  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u https://link.springer.com/article/10.1007/s00414-021-02673-1  |x Verlag  |z kostenfrei  |3 Volltext 
951 |a AR 
992 |a 20221214 
993 |a Article 
994 |a 2022 
998 |g 141981334  |a Weissenberger, Melanie  |m 141981334:Weissenberger, Melanie  |d 910000  |d 912100  |e 910000PW141981334  |e 912100PW141981334  |k 0/910000/  |k 1/910000/912100/  |p 2  |y j 
998 |g 1275511740  |a Burgardt, Nastasja  |m 1275511740:Burgardt, Nastasja  |d 910000  |d 912100  |e 910000PB1275511740  |e 912100PB1275511740  |k 0/910000/  |k 1/910000/912100/  |p 1  |x j 
999 |a KXP-PPN1827025557  |e 423100241X 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"First experiences with the Spectrum Compact CE System","title":"First experiences with the Spectrum Compact CE System"}],"person":[{"given":"Nastasja","family":"Burgardt","role":"aut","display":"Burgardt, Nastasja","roleDisplay":"VerfasserIn"},{"family":"Weissenberger","given":"Melanie","roleDisplay":"VerfasserIn","display":"Weissenberger, Melanie","role":"aut"}],"language":["eng"],"recId":"1827025557","type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Online veröffentlicht am 19. Oktober 2021","Gesehen am 14.12.2022"],"id":{"doi":["10.1007/s00414-021-02673-1"],"eki":["1827025557"]},"origin":[{"dateIssuedDisp":"2022","dateIssuedKey":"2022"}],"name":{"displayForm":["Nastasja Burgardt, Melanie Weissenberger"]},"relHost":[{"origin":[{"publisherPlace":"Berlin ; Heidelberg ; Getzville, NY ; Berlin ; Heidelberg ; New York, NY","publisher":"Springer ; HeinOnline ; Springer","dateIssuedKey":"1990","dateIssuedDisp":"1990-"}],"id":{"issn":["1437-1596"],"eki":["253724295"],"zdb":["1459222-8"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"International journal of legal medicine","title":"International journal of legal medicine","subtitle":"official publication of the International Academy of Legal Medicine"}],"note":["Gesehen am 06.03.25","Volltext auch als Teil einer Datenbank verfügbar"],"disp":"First experiences with the Spectrum Compact CE SystemInternational journal of legal medicine","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng","ger"],"recId":"253724295","pubHistory":["Volume 104, issue 1 (January 1990)-"],"titleAlt":[{"title":"Legal medicine"}],"part":{"volume":"136","text":"136(2022), 1, Seite 55-72","extent":"17","year":"2022","pages":"55-72","issue":"1"}}],"physDesc":[{"extent":"17 S."}]} 
SRT |a BURGARDTNAFIRSTEXPER2022 

Ähnliche Einträge