Cover Image

Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion

The Ras oncogene is known to activate three major MAPK pathways, ERK, JNK, p38 and exert distinct cellular phenotypes, that is, apoptosis and invasion through the Ras-MKK3-p38-signaling cascade. We attempted to identify the molecular targets of this pathway that selectively govern the invasive pheno...

Full description

Saved in:
Bibliographic Details
Main Authors: Behren, Andreas (Author) , Mühlen, Sabrina (Author) , Acuña Sanhueza, Gustavo A. (Author) , Schwager, Christian (Author) , Plinkert, Peter K. (Author) , Huber, Peter E. (Author) , Abdollahi, Amir (Author) , Simon, Christian (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: Oncogene
Year: 2010, Volume: 29, Issue: 10, Pages: 1519-1530
ISSN:1476-5594
DOI:10.1038/onc.2009.436
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/onc.2009.436
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/onc2009436
Get full text
Author Notes:A. Behren, S. Mühlen, G.A. Acuna Sanhueza, C. Schwager, P.K. Plinkert, P.E. Huber, A. Abdollahi and C. Simon

MARC

LEADER 00000caa a2200000 c 4500
001 1830365509
003 DE-627
005 20230710161926.0
007 cr uuu---uuuuu
008 230104s2010 xx |||||o 00| ||eng c
024 7 |a 10.1038/onc.2009.436  |2 doi 
035 |a (DE-627)1830365509 
035 |a (DE-599)KXP1830365509 
035 |a (OCoLC)1389822174 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Behren, Andreas  |d 1977-  |e VerfasserIn  |0 (DE-588)132430231  |0 (DE-627)522694527  |0 (DE-576)256600511  |4 aut 
245 1 0 |a Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion  |c A. Behren, S. Mühlen, G.A. Acuna Sanhueza, C. Schwager, P.K. Plinkert, P.E. Huber, A. Abdollahi and C. Simon 
264 1 |c 2010 
300 |a 12 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Published online 21 December 2009 
500 |a Gesehen am 04.01.2023 
520 |a The Ras oncogene is known to activate three major MAPK pathways, ERK, JNK, p38 and exert distinct cellular phenotypes, that is, apoptosis and invasion through the Ras-MKK3-p38-signaling cascade. We attempted to identify the molecular targets of this pathway that selectively govern the invasive phenotype. Stable transfection of NIH3T3 fibroblasts with MKK3act cDNA construct revealed similar p38-dependent in vitro characteristics observed in Ha-RasEJ-transformed NIH3T3 cells, including enhanced invasiveness and anchorage-independent growth correlating with p38 phosphorylation status. To identify the consensus downstream targets of the Ras-MKK3-p38 cascade involved in invasion, in vitro invasion assays were used to isolate highly invasive cells from both, MKK3 and Ha-RasEJ transgenic cell lines. Subsequently a genome-wide transcriptome analysis was employed to investigate differentially regulated genes in invasive Ha-RasEJ- and MKK3act-transfected NIH3T3 fibroblasts. Using this phenotype-assisted approach combined with system level protein-interaction network analysis, we identified FOXM1, PLK1 and CDK1 to be differentially regulated in invasive Ha-RasEJ-NIH3T3 and MKK3act-NIH3T3 cells. Finally, a FOXM1 RNA-knockdown approach revealed its requirement for both invasion and anchorage-independent growth of Ha-RasEJ- and MKK3act-NIH3T3 cells. Together, we identified FOXM1 as a key downstream target of Ras and MKK3-induced cellular in vitro invasion and anchorage-independent growth signaling. 
650 4 |a Apoptosis 
650 4 |a Cell Biology 
650 4 |a general 
650 4 |a Human Genetics 
650 4 |a Internal Medicine 
650 4 |a Medicine/Public Health 
650 4 |a Oncology 
700 1 |a Mühlen, Sabrina  |e VerfasserIn  |0 (DE-588)137004052  |0 (DE-627)589006827  |0 (DE-576)301386161  |4 aut 
700 1 |a Acuña Sanhueza, Gustavo A.  |d 1976-  |e VerfasserIn  |0 (DE-588)137926049  |0 (DE-627)598157298  |0 (DE-576)305875647  |4 aut 
700 1 |a Schwager, Christian  |e VerfasserIn  |0 (DE-588)1156925916  |0 (DE-627)1019872837  |0 (DE-576)502507187  |4 aut 
700 1 |a Plinkert, Peter K.  |e VerfasserIn  |0 (DE-588)1032658509  |0 (DE-627)739933043  |0 (DE-576)169008908  |4 aut 
700 1 |a Huber, Peter E.  |d 1965-  |e VerfasserIn  |0 (DE-588)1074678869  |0 (DE-627)832350109  |0 (DE-576)442767803  |4 aut 
700 1 |a Abdollahi, Amir  |e VerfasserIn  |0 (DE-588)129612715  |0 (DE-627)474757765  |0 (DE-576)297748874  |4 aut 
700 1 |a Simon, Christian  |e VerfasserIn  |0 (DE-588)1076385427  |0 (DE-627)834823616  |0 (DE-576)445275553  |4 aut 
773 0 8 |i Enthalten in  |t Oncogene  |d London : Springer Nature, 1997  |g 29(2010), 10, Seite 1519-1530  |h Online-Ressource  |w (DE-627)320469964  |w (DE-600)2008404-3  |w (DE-576)096188715  |x 1476-5594  |7 nnas  |a Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion 
773 1 8 |g volume:29  |g year:2010  |g number:10  |g pages:1519-1530  |g extent:12  |a Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion 
856 4 0 |u https://doi.org/10.1038/onc.2009.436  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.nature.com/articles/onc2009436  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20230104 
993 |a Article 
994 |a 2010 
998 |g 1076385427  |a Simon, Christian  |m 1076385427:Simon, Christian  |d 60000  |d 62100  |e 60000PS1076385427  |e 62100PS1076385427  |k 0/60000/  |k 1/60000/62100/  |p 8  |y j 
998 |g 129612715  |a Abdollahi, Amir  |m 129612715:Abdollahi, Amir  |d 910000  |d 911400  |e 910000PA129612715  |e 911400PA129612715  |k 0/910000/  |k 1/910000/911400/  |p 7 
998 |g 1074678869  |a Huber, Peter E.  |m 1074678869:Huber, Peter E.  |d 50000  |e 50000PH1074678869  |k 0/50000/  |p 6 
998 |g 1032658509  |a Plinkert, Peter K.  |m 1032658509:Plinkert, Peter K.  |d 60000  |d 62100  |e 60000PP1032658509  |e 62100PP1032658509  |k 0/60000/  |k 1/60000/62100/  |p 5 
998 |g 1156925916  |a Schwager, Christian  |m 1156925916:Schwager, Christian  |d 910000  |d 950000  |d 950400  |e 910000PS1156925916  |e 950000PS1156925916  |e 950400PS1156925916  |k 0/910000/  |k 1/910000/950000/  |k 2/910000/950000/950400/  |p 4 
999 |a KXP-PPN1830365509  |e 4244195483 
BIB |a Y 
SER |a journal 
JSO |a {"id":{"eki":["1830365509"],"doi":["10.1038/onc.2009.436"]},"recId":"1830365509","type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedKey":"2010","dateIssuedDisp":"2010"}],"name":{"displayForm":["A. Behren, S. Mühlen, G.A. Acuna Sanhueza, C. Schwager, P.K. Plinkert, P.E. Huber, A. Abdollahi and C. Simon"]},"note":["Published online 21 December 2009","Gesehen am 04.01.2023"],"language":["eng"],"person":[{"given":"Andreas","role":"aut","family":"Behren","display":"Behren, Andreas"},{"given":"Sabrina","display":"Mühlen, Sabrina","role":"aut","family":"Mühlen"},{"given":"Gustavo A.","display":"Acuña Sanhueza, Gustavo A.","family":"Acuña Sanhueza","role":"aut"},{"family":"Schwager","role":"aut","display":"Schwager, Christian","given":"Christian"},{"given":"Peter K.","display":"Plinkert, Peter K.","role":"aut","family":"Plinkert"},{"display":"Huber, Peter E.","role":"aut","family":"Huber","given":"Peter E."},{"family":"Abdollahi","role":"aut","display":"Abdollahi, Amir","given":"Amir"},{"display":"Simon, Christian","family":"Simon","role":"aut","given":"Christian"}],"relHost":[{"pubHistory":["Nachgewiesen 14.1997 -"],"note":["Gesehen am 18.03.05"],"language":["eng"],"disp":"Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasionOncogene","origin":[{"publisher":"Springer Nature ; Nature Publ. Group","dateIssuedKey":"1997","publisherPlace":"London ; Basingstoke","dateIssuedDisp":"1997-"}],"recId":"320469964","id":{"issn":["1476-5594"],"zdb":["2008404-3"],"eki":["320469964"]},"type":{"bibl":"periodical","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"including Oncogene reviews","title_sort":"Oncogene","title":"Oncogene"}],"part":{"text":"29(2010), 10, Seite 1519-1530","volume":"29","year":"2010","pages":"1519-1530","issue":"10","extent":"12"}}],"physDesc":[{"extent":"12 S."}],"title":[{"title":"Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion","title_sort":"Phenotype-assisted transcriptome analysis identifies FOXM1 downstream from Ras-MKK3-p38 to regulate in vitro cellular invasion"}]} 
SRT |a BEHRENANDRPHENOTYPEA2010 

Similar Items