Efficacy of an antibiotic loaded ceramic-based bone graft substitute for the treatment of infected non-unions
The treatment of non-unions is often complicated by segmental bone defects and bacterial colonization. Because of the limited availability of autologous bone grafts, tissue engineering focuses on antibiotic-loaded bone graft substitutes. HACaS+G is a resorbable calcium sulphate-hydroxyapatite loaded...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
8 October 2022
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| In: |
Biomedicines
Year: 2022, Volume: 10, Issue: 10, Pages: 1-17 |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines10102513 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/biomedicines10102513 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2227-9059/10/10/2513 
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| Author Notes: | Holger Freischmidt, Jonas Armbruster, Catharina Rothhaas, Nadine Titze, Thorsten Guehring, Dennis Nurjadi, Jan Philippe Kretzer, Gerhard Schmidmaier, Paul Alfred Grützner and Lars Helbig |
| Summary: | The treatment of non-unions is often complicated by segmental bone defects and bacterial colonization. Because of the limited availability of autologous bone grafts, tissue engineering focuses on antibiotic-loaded bone graft substitutes. HACaS+G is a resorbable calcium sulphate-hydroxyapatite loaded with gentamicin. The osteoinductive, osteoconductive, and anti-infective effect of HACaS+G has already been demonstrated in clinical studies on patients with chronic osteomyelitis. However, especially for the treatment of infected non-unions with segmental bone defects by HACaS+G, reliable clinical testing is difficult and sufficient experimental data are lacking. We used an already established sequential animal model in infected and non-infected rat femora to investigate the osteoinductive, osteoconductive, and anti-infective efficacy of HACaS+G for the treatment of infected non-unions. In biomechanical testing, bone consolidation could not be observed under infected and non-infected conditions. Only a prophylactic effect against infections, but no eradication, could be verified in the microbiological analysis. Using µ-CT scans and histology, osteoinduction was detected in both the infected and non-infected bone, whereas osteoconduction occurred only in the non-infected setting. Our data showed that HACaS+G is osteoinductive, but does not have added benefits in infected non-unions in terms of osteoconduction and mechanical bone stability, especially in those with segmental bone defects. |
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| Item Description: | Gesehen am 17.01.2023 |
| Physical Description: | Online Resource |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines10102513 |