Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids
Analyses of G-protein-mediated contraction and relaxation of vascular smooth muscle cells (VSMCs) are usually hampered by a rigid growth surface and culture conditions promoting cell proliferation and a less contractile phenotype. Our studies indicated that mouse aortic VSMCs cultured in three-dimen...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2023
|
| In: |
Cells
Year: 2023, Jahrgang: 12, Heft: 1, Pages: 1-15 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells12010128 |
| Online-Zugang: | Resolving-System, Volltext: https://doi.org/10.3390/cells12010128 Verlag, Volltext: https://www.mdpi.com/2073-4409/12/1/128 
|
| Verfasserangaben: | Jaspal Garg, Alexandra Sporkova, Markus Hecker and Thomas Korff |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 183290959X | ||
| 003 | DE-627 | ||
| 005 | 20230706221439.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230201s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cells12010128 |2 doi | |
| 035 | |a (DE-627)183290959X | ||
| 035 | |a (DE-599)KXP183290959X | ||
| 035 | |a (OCoLC)1389533233 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Garg, Jaspal |e VerfasserIn |0 (DE-588)1136090185 |0 (DE-627)891232001 |0 (DE-576)490306845 |4 aut | |
| 245 | 1 | 0 | |a Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids |c Jaspal Garg, Alexandra Sporkova, Markus Hecker and Thomas Korff |
| 264 | 1 | |c 2023 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht: 28 December 2022 | ||
| 500 | |a Gesehen am 01.02.2023 | ||
| 520 | |a Analyses of G-protein-mediated contraction and relaxation of vascular smooth muscle cells (VSMCs) are usually hampered by a rigid growth surface and culture conditions promoting cell proliferation and a less contractile phenotype. Our studies indicated that mouse aortic VSMCs cultured in three-dimensional spheroids acquire a quiescent contractile status while decreasing the baseline G-protein-dependent inositolphosphate formation and increasing the expression of endothelin receptor type A (Ednra). Endothelin-1 (ET-1) promoted inositolphosphate formation in VSMC spheroids, but not in VSMCs cultured under standard conditions. To trace ET-1-mediated contraction of VSMC spheroids, we developed an assay by adhering them to collagen hydrogels and recording structural changes by time-lapse microscopy. Under these conditions, mouse and human VSMC spheroids contracted upon treatment with ET-1 and potassium chloride or relaxed in response to caffeine and the prostacyclin analogue Iloprost. ET-1 activated AKT-, MKK1-, and MKK3/6-dependent signaling cascades, which were inhibited by an overexpressing regulator of G-protein signaling 5 (Rgs5) to terminate the activity of Gα subunits. In summary, culture of VSMCs in three-dimensional spheroids lowers baseline G-protein activity and enables analyses of both contraction and relaxation of mouse and human VSMCs. This model serves as a simple and versatile tool for drug testing and investigating G-protein-depending signaling. | ||
| 650 | 4 | |a contraction | |
| 650 | 4 | |a G-protein signaling | |
| 650 | 4 | |a MAPK | |
| 650 | 4 | |a relaxation | |
| 650 | 4 | |a RGS | |
| 650 | 4 | |a VSMC phenotype | |
| 700 | 1 | |a Sporkova, Alexandra |e VerfasserIn |0 (DE-588)1247776506 |0 (DE-627)1782386076 |4 aut | |
| 700 | 1 | |a Hecker, Markus |d 1960- |e VerfasserIn |0 (DE-588)1071693344 |0 (DE-627)826266037 |0 (DE-576)433260319 |4 aut | |
| 700 | 1 | |a Korff, Thomas |e VerfasserIn |0 (DE-588)1048734951 |0 (DE-627)781211824 |0 (DE-576)386952604 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cells |d Basel : MDPI, 2012 |g 12(2023), 1, Artikel-ID 128, Seite 1-15 |h Online-Ressource |w (DE-627)718622081 |w (DE-600)2661518-6 |w (DE-576)365412112 |x 2073-4409 |7 nnas |a Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids |
| 773 | 1 | 8 | |g volume:12 |g year:2023 |g number:1 |g elocationid:128 |g pages:1-15 |g extent:15 |a Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cells12010128 |x Resolving-System |x Verlag |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2073-4409/12/1/128 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230201 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1048734951 |a Korff, Thomas |m 1048734951:Korff, Thomas |d 50000 |d 53200 |d 50000 |e 50000PK1048734951 |e 53200PK1048734951 |e 50000PK1048734951 |k 0/50000/ |k 1/50000/53200/ |k 0/50000/ |p 4 |y j | ||
| 998 | |g 1071693344 |a Hecker, Markus |m 1071693344:Hecker, Markus |d 50000 |d 53200 |e 50000PH1071693344 |e 53200PH1071693344 |k 0/50000/ |k 1/50000/53200/ |p 3 | ||
| 998 | |g 1247776506 |a Sporkova, Alexandra |m 1247776506:Sporkova, Alexandra |d 50000 |d 53200 |e 50000PS1247776506 |e 53200PS1247776506 |k 0/50000/ |k 1/50000/53200/ |p 2 | ||
| 998 | |g 1136090185 |a Garg, Jaspal |m 1136090185:Garg, Jaspal |d 50000 |d 53200 |e 50000PG1136090185 |e 53200PG1136090185 |k 0/50000/ |k 1/50000/53200/ |p 1 |x j | ||
| 999 | |a KXP-PPN183290959X |e 4261092719 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids","title_sort":"Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids"}],"person":[{"family":"Garg","display":"Garg, Jaspal","roleDisplay":"VerfasserIn","role":"aut","given":"Jaspal"},{"given":"Alexandra","role":"aut","display":"Sporkova, Alexandra","family":"Sporkova","roleDisplay":"VerfasserIn"},{"given":"Markus","role":"aut","family":"Hecker","display":"Hecker, Markus","roleDisplay":"VerfasserIn"},{"role":"aut","given":"Thomas","roleDisplay":"VerfasserIn","family":"Korff","display":"Korff, Thomas"}],"note":["Online veröffentlicht: 28 December 2022","Gesehen am 01.02.2023"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"15 S."}],"language":["eng"],"id":{"eki":["183290959X"],"doi":["10.3390/cells12010128"]},"recId":"183290959X","relHost":[{"origin":[{"publisherPlace":"Basel","dateIssuedKey":"2012","dateIssuedDisp":"2012-","publisher":"MDPI"}],"recId":"718622081","pubHistory":["1.2012 -"],"disp":"Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroidsCells","id":{"eki":["718622081"],"issn":["2073-4409"],"zdb":["2661518-6"]},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 09.05.12"],"part":{"volume":"12","pages":"1-15","year":"2023","issue":"1","extent":"15","text":"12(2023), 1, Artikel-ID 128, Seite 1-15"},"title":[{"title_sort":"Cells","title":"Cells","subtitle":"open access journal"}]}],"name":{"displayForm":["Jaspal Garg, Alexandra Sporkova, Markus Hecker and Thomas Korff"]},"origin":[{"dateIssuedDisp":"2023","dateIssuedKey":"2023"}]} | ||
| SRT | |a GARGJASPALTRACINGGPR2023 | ||