Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography
The application of optical projection tomography to in-vivo experiments is limited by specimen movement during the acquisition. We present a set of mathematical correction methods applied to the acquired data stacks to correct for movement in both directions of the image plane. These methods have be...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
14 Jul 2010
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| In: |
Biomedical optics express
Year: 2010, Volume: 1, Issue: 1, Pages: 87-96 |
| ISSN: | 2156-7085 |
| Online Access: |
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| Author Notes: | Udo Jochen Birk, Matthias Rieckher, Nikos Konstantinides, Alex Darrell, Ana Sarasa-Renedo, Heiko Meyer, Nektarios Tavernarakis, and Jorge Ripoll |
| Summary: | The application of optical projection tomography to in-vivo experiments is limited by specimen movement during the acquisition. We present a set of mathematical correction methods applied to the acquired data stacks to correct for movement in both directions of the image plane. These methods have been applied to correct experimental data taken from in-vivo optical projection tomography experiments in Caenorhabditis elegans. Successful reconstructions for both fluorescence and white light (absorption) measurements are shown. Since no difference between movement of the animal and movement of the rotation axis is made, this approach at the same time removes artifacts due to mechanical drifts and errors in the assumed center of rotation. |
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| Item Description: | Gesehen am 06.02.2023 |
| Physical Description: | Online Resource |
| ISSN: | 2156-7085 |