Transplantation of TAT-Bcl-xL-transduced neural precursor cells: long-term neuroprotection after stroke
Neural precursor cells (NPC) are an interesting tool in experimental stroke research, but their therapeutic potential is limited due to poor long-term survival. We therefore in vitro transduced subventricular zone-(SVZ)-derived NPC with the anti-apoptotic fusion protein TAT-Bcl-xL and analyzed NPC s...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 June 2010
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| In: |
Neurobiology of disease
Year: 2010, Jahrgang: 40, Heft: 1, Pages: 265-276 |
| ISSN: | 1095-953X |
| DOI: | 10.1016/j.nbd.2010.05.033 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.nbd.2010.05.033 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0969996110001956 
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| Verfasserangaben: | Thorsten R. Doeppner, Mimount El Aanbouri, Gunnar P.H. Dietz, Jens Weise, Sönke Schwarting, Mathias Bähr |
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| 245 | 1 | 0 | |a Transplantation of TAT-Bcl-xL-transduced neural precursor cells |b long-term neuroprotection after stroke |c Thorsten R. Doeppner, Mimount El Aanbouri, Gunnar P.H. Dietz, Jens Weise, Sönke Schwarting, Mathias Bähr |
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| 520 | |a Neural precursor cells (NPC) are an interesting tool in experimental stroke research, but their therapeutic potential is limited due to poor long-term survival. We therefore in vitro transduced subventricular zone-(SVZ)-derived NPC with the anti-apoptotic fusion protein TAT-Bcl-xL and analyzed NPC survival, differentiation, and post-stroke functional deficits after experimental ischemia in mice. Survival of TAT-Bcl-xL-transduced NPC, which were injected at day 7 post-stroke into the ischemic striatum, was significantly increased at 4weeks after stroke. Increased survival of NPC was associated with reduced infarct injury and decreased post-stroke functional deficits. Animals grafted with TAT-Bcl-xL-transduced NPC showed an increased number of immature cells expressing the neuronal marker doublecortin. Since mature neuronal differentiation of NPC was not observed, reduced post-stroke injury cannot be attributed to enhanced neuronal regeneration, but rather to indirect by-stander effects of grafted NPC. In line with this, NPC-mediated neuroprotection of cortical neurons in vitro was associated with increased secretion of growth factors. Thus, in vitro transduction of cultivated NPC with TAT-Bcl-xL results in enhanced resistance of transplanted NPC followed by long-term neuroprotection and ameliorated functional deficits after transient focal cerebral ischemia in mice. | ||
| 650 | 4 | |a Cerebral ischemia | |
| 650 | 4 | |a Neural precursor cells | |
| 650 | 4 | |a Neurogenesis | |
| 650 | 4 | |a TAT-Bcl-x | |
| 650 | 4 | |a Ttroke | |
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| 700 | 1 | |a Dietz, Gunnar P. H. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Weise, Jens |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Bähr, Mathias |e VerfasserIn |4 aut | |
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