cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins
The biosynthesis of most membrane proteins is directly coupled to membrane insertion, and therefore, molecular chaperones are not required. The light-harvesting chlorophyll a,b-binding proteins (LHCPs) present a prominent exception as they are synthesized in the cytoplasm, and after import into the...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 24, 2010
|
| In: |
The journal of biological chemistry
Year: 2010, Jahrgang: 285, Heft: 28, Pages: 21655-21661 |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.C110.132746 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1074/jbc.C110.132746 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0021925820601934 
|
| Verfasserangaben: | Sebastian Falk, Irmgard Sinning |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1834594456 | ||
| 003 | DE-627 | ||
| 005 | 20230710160337.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230213s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/jbc.C110.132746 |2 doi | |
| 035 | |a (DE-627)1834594456 | ||
| 035 | |a (DE-599)KXP1834594456 | ||
| 035 | |a (OCoLC)1389821740 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Falk, Sebastian |e VerfasserIn |0 (DE-588)1018087737 |0 (DE-627)690513798 |0 (DE-576)353800082 |4 aut | |
| 245 | 1 | 0 | |a cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins |c Sebastian Falk, Irmgard Sinning |
| 264 | 1 | |c May 24, 2010 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 13.02.2023 | ||
| 520 | |a The biosynthesis of most membrane proteins is directly coupled to membrane insertion, and therefore, molecular chaperones are not required. The light-harvesting chlorophyll a,b-binding proteins (LHCPs) present a prominent exception as they are synthesized in the cytoplasm, and after import into the chloroplast, they are targeted and inserted into the thylakoid membrane. Upon arrival in the stroma, LHCPs form a soluble transit complex with the chloroplast signal recognition particle (cpSRP) consisting of an SRP54 homolog and the unique cpSRP43 composed of three chromodomains and four ankyrin repeats. Here we describe that cpSRP43 alone prevents aggregation of LHCP by formation of a complex with nanomolar affinity, whereas cpSRP54 is not required for this chaperone activity. Other stromal chaperones like trigger factor cannot replace cpSRP43, which implies that LHCPs require a specific chaperone. Although cpSRP43 does not have an ATPase activity, it can dissolve aggregates of LHCPs similar to chaperones of the Hsp104/ClpB family. We show that the LHCP-cpSRP43 interaction is predominantly hydrophobic but strictly depends on an intact DPLG motif between the second and third transmembrane region. The cpSRP43 ankyrin repeats that provide the binding site for the DPLG motif are sufficient for the chaperone function, whereas the chromodomains are dispensable. Taken together, we define cpSRP43 as a highly specific chaperone for LHCPs in addition to its established function as a targeting factor for this family of membrane protein. | ||
| 650 | 4 | |a Amino Acid Motifs | |
| 650 | 4 | |a Ankyrins | |
| 650 | 4 | |a Binding Sites | |
| 650 | 4 | |a Cell Membrane | |
| 650 | 4 | |a Chlorophyll | |
| 650 | 4 | |a Chloroplast Proteins | |
| 650 | 4 | |a Chloroplasts | |
| 650 | 4 | |a Heat-Shock Proteins | |
| 650 | 4 | |a Light-Harvesting Protein Complexes | |
| 650 | 4 | |a Molecular Chaperones | |
| 650 | 4 | |a Photosynthesis | |
| 650 | 4 | |a Plant Proteins | |
| 650 | 4 | |a Protein Binding | |
| 650 | 4 | |a Protein Folding | |
| 650 | 4 | |a Protein Structure, Tertiary | |
| 650 | 4 | |a Signal Recognition Particle | |
| 700 | 1 | |a Sinning, Irmgard |d 1960- |e VerfasserIn |0 (DE-588)1027598617 |0 (DE-627)72945133X |0 (DE-576)166290580 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of biological chemistry |d [Amsterdam] : Elsevier B.V., 1905 |g 285(2010), 28, Seite 21655-21661 |h Online-Ressource |w (DE-627)269247025 |w (DE-600)1474604-9 |w (DE-576)077883837 |x 1083-351X |7 nnas |a cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins |
| 773 | 1 | 8 | |g volume:285 |g year:2010 |g number:28 |g pages:21655-21661 |g extent:7 |a cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins |
| 856 | 4 | 0 | |u https://doi.org/10.1074/jbc.C110.132746 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0021925820601934 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230213 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1027598617 |a Sinning, Irmgard |m 1027598617:Sinning, Irmgard |d 700000 |d 711000 |e 700000PS1027598617 |e 711000PS1027598617 |k 0/700000/ |k 1/700000/711000/ |p 2 |y j | ||
| 998 | |g 1018087737 |a Falk, Sebastian |m 1018087737:Falk, Sebastian |d 140000 |e 140000PF1018087737 |k 0/140000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1834594456 |e 4271149071 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins","title":"cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1074/jbc.C110.132746"],"eki":["1834594456"]},"physDesc":[{"extent":"7 S."}],"relHost":[{"title":[{"title":"The journal of biological chemistry","subtitle":"JBC","title_sort":"journal of biological chemistry"}],"id":{"zdb":["1474604-9"],"eki":["269247025"],"issn":["1083-351X"]},"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisher":"Elsevier B.V. ; American Soc. of Biological Chemists ; ASBMB Publications","dateIssuedDisp":"2021-","dateIssuedKey":"2021","publisherPlace":"[Amsterdam] ; Baltimore, Md. [u.a.] ; Bethesda, Md."}],"name":{"displayForm":["publ. by the American Society for Biochemistry and Molecular Biology"]},"recId":"269247025","corporate":[{"display":"American Society for Biochemistry and Molecular Biology","role":"isb"}],"disp":"cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteinsThe journal of biological chemistry","type":{"bibl":"periodical","media":"Online-Ressource"},"titleAlt":[{"title":"JBC online"},{"title":"JBC"}],"language":["eng"],"part":{"text":"285(2010), 28, Seite 21655-21661","extent":"7","pages":"21655-21661","issue":"28","year":"2010","volume":"285"},"note":["Gesehen am 19.05.2021"],"pubHistory":["1.1905/06 -"]}],"origin":[{"dateIssuedKey":"2010","dateIssuedDisp":"May 24, 2010"}],"name":{"displayForm":["Sebastian Falk, Irmgard Sinning"]},"language":["eng"],"recId":"1834594456","person":[{"role":"aut","family":"Falk","display":"Falk, Sebastian","given":"Sebastian"},{"role":"aut","family":"Sinning","display":"Sinning, Irmgard","given":"Irmgard"}],"note":["Gesehen am 13.02.2023"]} | ||
| SRT | |a FALKSEBASTCPSRP43ISA2420 | ||