Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas
Aberrant wnt signaling caused by mutations in CTNNB1 occurs in about 15% of Wilms tumors, and these mutations appear to be dependent on the concomitant mutational inactivation of the zinc-finger protein WT1. Nuclear ?-catenin protein, a substitute marker of active wnt signaling, has been detected in...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2010
|
| In: |
Pediatric and developmental pathology
Year: 2010, Volume: 13, Issue: 1, Pages: 1-8 |
| ISSN: | 1615-5742 |
| DOI: | 10.2350/08-03-0443.1 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2350/08-03-0443.1
|
| Author Notes: | David Ehrlich, Elisabeth Bruder, Martin A. Thome, Carsten N. Gutt, Magnus von Knebel Doeberitz, Felix Niggli, Alan O. Perantoni, and Robert Koesters |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1837940843 | ||
| 003 | DE-627 | ||
| 005 | 20230710154512.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230302s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.2350/08-03-0443.1 |2 doi | |
| 035 | |a (DE-627)1837940843 | ||
| 035 | |a (DE-599)KXP1837940843 | ||
| 035 | |a (OCoLC)1389821270 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Ehrlich, David Hans Wolf |d 1977- |e VerfasserIn |0 (DE-588)140974156 |0 (DE-627)703846108 |0 (DE-576)321618718 |4 aut | |
| 245 | 1 | 0 | |a Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas |c David Ehrlich, Elisabeth Bruder, Martin A. Thome, Carsten N. Gutt, Magnus von Knebel Doeberitz, Felix Niggli, Alan O. Perantoni, and Robert Koesters |
| 246 | 3 | 3 | |a Nuclear accumulation of beta-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas |
| 264 | 1 | |c 2010 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published online: April 6, 2009 | ||
| 500 | |a Gesehen am 02.03.2023 | ||
| 520 | |a Aberrant wnt signaling caused by mutations in CTNNB1 occurs in about 15% of Wilms tumors, and these mutations appear to be dependent on the concomitant mutational inactivation of the zinc-finger protein WT1. Nuclear ?-catenin protein, a substitute marker of active wnt signaling, has been detected in an even higher proportion (>50%) of Wilms tumors, suggesting alternative genetic pathways leading to ?-catenin activation. Thus, targeting wnt signaling may become an important future therapeutic strategy in Wilms tumor patients. Currently, chemically induced rat nephroblastomas provide the only available rodent model for this tumor. To determine the contribution of active wnt signaling in this model, we investigated 24 chemically induced rat nephroblastomas for ?-catenin protein expression and for Ctnnb1 and WT1 mutations. Immunohistochemistry showed focal strong nuclear accumulation of ?-catenin protein in 18 of 24 tumors, although in a heterogenous pattern. Blastemal and mesenchymal compartments displayed nuclear-positive cells more frequently than areas of epithelial differentiation. Interestingly, we found no mutation of exon 3 of Ctnnb1 and no mutation within the zinc-finger region of WT1 in any of the 24 tumors analyzed. In conclusion, our findings suggest activation of wnt signaling in the majority (63%) of chemically induced rat nephroblastomas. Nuclear expression of ?-catenin in the absence of Ctnnb1 mutations implies, however, alternate mutational targets in rat nephroblastomas. | ||
| 700 | 1 | |a Bruder, Elisabeth |e VerfasserIn |4 aut | |
| 700 | 1 | |a Thome, Martin Andreas |d 1976- |e VerfasserIn |0 (DE-588)136362419 |0 (DE-627)581102118 |0 (DE-576)300981619 |4 aut | |
| 700 | 1 | |a Gutt, Carsten N. |e VerfasserIn |0 (DE-588)1043329862 |0 (DE-627)770455948 |0 (DE-576)394646789 |4 aut | |
| 700 | 1 | |a Knebel Doeberitz, Magnus von |e VerfasserIn |0 (DE-588)1022165291 |0 (DE-627)71693289X |0 (DE-576)364934239 |4 aut | |
| 700 | 1 | |a Niggli, Felix |e VerfasserIn |4 aut | |
| 700 | 1 | |a Perantoni, Alan O. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Koesters, Robert |e VerfasserIn |0 (DE-588)1076838081 |0 (DE-627)835382222 |0 (DE-576)17111342X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pediatric and developmental pathology |d Thousand Oaks, CA : Sage Publishing, 1998 |g 13(2010), 1, Seite 1-8 |h Online-Ressource |w (DE-627)27159666X |w (DE-600)1480654-X |w (DE-576)088704343 |x 1615-5742 |7 nnas |a Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas |
| 773 | 1 | 8 | |g volume:13 |g year:2010 |g number:1 |g pages:1-8 |g extent:8 |a Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas |
| 856 | 4 | 0 | |u https://doi.org/10.2350/08-03-0443.1 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230302 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1022165291 |a Knebel Doeberitz, Magnus von |m 1022165291:Knebel Doeberitz, Magnus von |d 910000 |d 912000 |e 910000PK1022165291 |e 912000PK1022165291 |k 0/910000/ |k 1/910000/912000/ |p 5 | ||
| 998 | |g 140974156 |a Ehrlich, David Hans Wolf |m 140974156:Ehrlich, David Hans Wolf |d 50000 |e 50000PE140974156 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1837940843 |e 4282518952 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1837940843","language":["eng"],"note":["Published online: April 6, 2009","Gesehen am 02.03.2023"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"titleAlt":[{"title":"Nuclear accumulation of beta-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas"}],"person":[{"given":"David Hans Wolf","family":"Ehrlich","role":"aut","roleDisplay":"VerfasserIn","display":"Ehrlich, David Hans Wolf"},{"given":"Elisabeth","family":"Bruder","role":"aut","roleDisplay":"VerfasserIn","display":"Bruder, Elisabeth"},{"given":"Martin Andreas","family":"Thome","role":"aut","roleDisplay":"VerfasserIn","display":"Thome, Martin Andreas"},{"roleDisplay":"VerfasserIn","display":"Gutt, Carsten N.","role":"aut","family":"Gutt","given":"Carsten N."},{"display":"Knebel Doeberitz, Magnus von","roleDisplay":"VerfasserIn","role":"aut","family":"Knebel Doeberitz","given":"Magnus von"},{"given":"Felix","family":"Niggli","role":"aut","roleDisplay":"VerfasserIn","display":"Niggli, Felix"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Perantoni, Alan O.","given":"Alan O.","family":"Perantoni"},{"family":"Koesters","given":"Robert","display":"Koesters, Robert","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas","title_sort":"Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomas"}],"relHost":[{"origin":[{"publisherPlace":"Thousand Oaks, CA ; New York, NY ; Lawrence, KS","dateIssuedKey":"1998","publisher":"Sage Publishing ; Springer ; Allen Press","dateIssuedDisp":"1998-"}],"id":{"eki":["27159666X"],"zdb":["1480654-X"],"issn":["1615-5742"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"PDP : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society","title":"Pediatric and developmental pathology","title_sort":"Pediatric and developmental pathology"}],"note":["Erscheint alle 2 Monate","Fortsetzung der Druck-Ausgabe","Gesehen am 15.07.2023"],"disp":"Nuclear accumulation of β-catenin protein indicates activation of wnt signaling in chemically induced rat nephroblastomasPediatric and developmental pathology","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"recId":"27159666X","pubHistory":["1.1998 -"],"titleAlt":[{"title":"PDP"}],"part":{"volume":"13","text":"13(2010), 1, Seite 1-8","extent":"8","year":"2010","issue":"1","pages":"1-8"}}],"physDesc":[{"extent":"8 S."}],"name":{"displayForm":["David Ehrlich, Elisabeth Bruder, Martin A. Thome, Carsten N. Gutt, Magnus von Knebel Doeberitz, Felix Niggli, Alan O. Perantoni, and Robert Koesters"]},"id":{"eki":["1837940843"],"doi":["10.2350/08-03-0443.1"]},"origin":[{"dateIssuedKey":"2010","dateIssuedDisp":"2010"}]} | ||
| SRT | |a EHRLICHDAVNUCLEARACC2010 | ||