Buchumschlag

The nucleoporin RanBP2 has SUMO1 E3 ligase activity

Posttranslational modification with SUMO1 regulates protein/protein interactions, localization, and stability. SUMOylation requires the E1 enzyme Aos1/Uba2 and the E2 enzyme Ubc9. A family of E3-like factors, PIAS proteins, was discovered recently. Here we show that the nucleoporin RanBP2/Nup358 als...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Pichler, Andrea (VerfasserIn) , Gast, Andreas (VerfasserIn) , Seeler, Jacob S. (VerfasserIn) , Dejean, Anne (VerfasserIn) , Melchior, Frauke (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 11 January 2002
In: Cell
Year: 2002, Jahrgang: 108, Heft: 1, Pages: 109-120
ISSN:1097-4172
Online-Zugang: Volltext
Verfasserangaben:Andrea Pichler, Andreas Gast, Jacob S. Seeler, Anne Dejean, Frauke Melchior

MARC

LEADER 00000caa a2200000 c 4500
001 1838989803
003 DE-627
005 20230710173346.0
007 cr uuu---uuuuu
008 230313s2002 xx |||||o 00| ||eng c
024 7 |a 10.1016/s0092-8674(01)00633-x  |2 doi 
035 |a (DE-627)1838989803 
035 |a (DE-599)KXP1838989803 
035 |a (OCoLC)1389825626 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Pichler, Andrea  |d 1966-  |e VerfasserIn  |0 (DE-588)1107797195  |0 (DE-627)863624960  |0 (DE-576)475134044  |4 aut 
245 1 4 |a The nucleoporin RanBP2 has SUMO1 E3 ligase activity  |c Andrea Pichler, Andreas Gast, Jacob S. Seeler, Anne Dejean, Frauke Melchior 
264 1 |c 11 January 2002 
300 |a 12 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 13.03.2023 
520 |a Posttranslational modification with SUMO1 regulates protein/protein interactions, localization, and stability. SUMOylation requires the E1 enzyme Aos1/Uba2 and the E2 enzyme Ubc9. A family of E3-like factors, PIAS proteins, was discovered recently. Here we show that the nucleoporin RanBP2/Nup358 also has SUMO1 E3-like activity. RanBP2 directly interacts with the E2 enzyme Ubc9 and strongly enhances SUMO1-transfer from Ubc9 to the SUMO1 target Sp100. The E3-like activity is contained within a 33 kDa domain of RanBP2 that lacks RING finger motifs and does not resemble PIAS family proteins. Our findings place SUMOylation at the cytoplasmic filaments of the NPC and suggest that, at least for some substrates, modification and nuclear import are linked events. 
650 4 |a Animals 
650 4 |a Antibodies 
650 4 |a Antigens, Nuclear 
650 4 |a Autoantigens 
650 4 |a Bacterial Proteins 
650 4 |a Cell Nucleus 
650 4 |a Cytoplasm 
650 4 |a Goats 
650 4 |a GTPase-Activating Proteins 
650 4 |a Ligases 
650 4 |a Luminescent Proteins 
650 4 |a Molecular Chaperones 
650 4 |a Nuclear Pore Complex Proteins 
650 4 |a Nuclear Proteins 
650 4 |a Protein Processing, Post-Translational 
650 4 |a Rabbits 
650 4 |a SUMO-1 Protein 
650 4 |a Ubiquitin-Conjugating Enzymes 
650 4 |a Ubiquitin-Protein Ligases 
650 4 |a Zinc Fingers 
700 1 |a Gast, Andreas  |e VerfasserIn  |0 (DE-588)1226448496  |0 (DE-627)1747463841  |4 aut 
700 1 |a Seeler, Jacob S.  |e VerfasserIn  |4 aut 
700 1 |a Dejean, Anne  |e VerfasserIn  |4 aut 
700 1 |a Melchior, Frauke  |d 1962-  |e VerfasserIn  |0 (DE-588)1044947144  |0 (DE-627)773153624  |0 (DE-576)370602919  |4 aut 
773 0 8 |i Enthalten in  |t Cell  |d [Cambridge, Mass.] : Cell Press, 1974  |g 108(2002), 1, Seite 109-120  |h Online-Ressource  |w (DE-627)320416127  |w (DE-600)2001951-8  |w (DE-576)090881370  |x 1097-4172  |7 nnas  |a The nucleoporin RanBP2 has SUMO1 E3 ligase activity 
773 1 8 |g volume:108  |g year:2002  |g number:1  |g pages:109-120  |g extent:12  |a The nucleoporin RanBP2 has SUMO1 E3 ligase activity 
951 |a AR 
992 |a 20230313 
993 |a Article 
994 |a 2002 
998 |g 1044947144  |a Melchior, Frauke  |m 1044947144:Melchior, Frauke  |p 5  |y j 
998 |g 1226448496  |a Gast, Andreas  |m 1226448496:Gast, Andreas  |d 140000  |e 140000PG1226448496  |k 0/140000/  |p 2 
999 |a KXP-PPN1838989803  |e 4288534097 
BIB |a Y 
SER |a journal 
JSO |a {"note":["Gesehen am 13.03.2023"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1838989803","language":["eng"],"person":[{"roleDisplay":"VerfasserIn","display":"Pichler, Andrea","role":"aut","family":"Pichler","given":"Andrea"},{"display":"Gast, Andreas","roleDisplay":"VerfasserIn","role":"aut","family":"Gast","given":"Andreas"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Seeler, Jacob S.","given":"Jacob S.","family":"Seeler"},{"family":"Dejean","given":"Anne","roleDisplay":"VerfasserIn","display":"Dejean, Anne","role":"aut"},{"role":"aut","display":"Melchior, Frauke","roleDisplay":"VerfasserIn","given":"Frauke","family":"Melchior"}],"title":[{"title_sort":"nucleoporin RanBP2 has SUMO1 E3 ligase activity","title":"The nucleoporin RanBP2 has SUMO1 E3 ligase activity"}],"physDesc":[{"extent":"12 S."}],"relHost":[{"disp":"The nucleoporin RanBP2 has SUMO1 E3 ligase activityCell","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 11.03.24"],"language":["eng"],"recId":"320416127","pubHistory":["1.1974 -"],"part":{"extent":"12","volume":"108","text":"108(2002), 1, Seite 109-120","issue":"1","pages":"109-120","year":"2002"},"title":[{"title":"Cell","title_sort":"Cell"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"[Cambridge, Mass.] ; New York, NY","dateIssuedDisp":"1974-","publisher":"Cell Press ; Elsevier","dateIssuedKey":"1974"}],"id":{"eki":["320416127"],"zdb":["2001951-8"],"issn":["1097-4172"]}}],"name":{"displayForm":["Andrea Pichler, Andreas Gast, Jacob S. Seeler, Anne Dejean, Frauke Melchior"]},"origin":[{"dateIssuedKey":"2002","dateIssuedDisp":"11 January 2002"}],"id":{"doi":["10.1016/s0092-8674(01)00633-x"],"eki":["1838989803"]}} 
SRT |a PICHLERANDNUCLEOPORI1120 

Ähnliche Einträge