Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives
The use of in vitro 3-D cell culture models in cancer research has yielded substantial gains in knowledge on various aspects of tumour biology. Such cell culture models could be useful in the study of head and neck squamous cell carcinoma (HNSCC), where mimicking intratumoral and intertumoral hetero...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 2022
|
| In: |
Alternatives to laboratory animals
Year: 2022, Volume: 50, Issue: 6, Pages: 414-422 |
| ISSN: | 2632-3559 |
| DOI: | 10.1177/02611929221135042 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1177/02611929221135042
|
| Author Notes: | Esther Tenschert, Johann Kern, Annette Affolter, Nicole Rotter, and Anne Lammert |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1839065265 | ||
| 003 | DE-627 | ||
| 005 | 20230706230728.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230313s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1177/02611929221135042 |2 doi | |
| 035 | |a (DE-627)1839065265 | ||
| 035 | |a (DE-599)KXP1839065265 | ||
| 035 | |a (OCoLC)1389534386 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Tenschert, Esther |d 1994- |e VerfasserIn |0 (DE-588)1227495234 |0 (DE-627)1748493167 |4 aut | |
| 245 | 1 | 0 | |a Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives |c Esther Tenschert, Johann Kern, Annette Affolter, Nicole Rotter, and Anne Lammert |
| 264 | 1 | |c November 2022 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 13.03.2023 | ||
| 520 | |a The use of in vitro 3-D cell culture models in cancer research has yielded substantial gains in knowledge on various aspects of tumour biology. Such cell culture models could be useful in the study of head and neck squamous cell carcinoma (HNSCC), where mimicking intratumoral and intertumoral heterogeneity is especially challenging. Our research aims to establish 3-D spheroid models for HNSCC that reproduce in vitro the connections between tumour cells and the surrounding microenvironment. The aims of this study were to determine the optimal conditions for the culture and use of spheroids from HNSCC cell lines and optimal timepoint for using the spheroids obtained, to evaluate the effects of coculture with tumour-specific fibroblasts on spheroid formation, and to investigate spheroid responses to cisplatin treatment. Four HNSCC cell lines (UMSCC-11A, UMSCC-11B, UMSCC-22B and UD-SCC-01) were seeded in flat or round bottom well ultra-low attachment spheroid plates, and spheroid formation was evaluated. The HNSCC cell lines were then cocultured with stromal cells of the tumour microenvironment, producing an accelerated formation of dense spheroids. The viability of cells within the spheroids was assessed during cell culture by using a fluorescent dye. Our results suggest that: three out of the four cell lines tested could form usable spheroids with acceptable viability; the addition of stromal cells did not improve the number of viable cells; and the use of round bottom well plates supported the formation of a single spheroid, whereas flat bottom well plates led to the formation of multiple spheroids of different sizes. | ||
| 650 | 4 | |a cancer | |
| 650 | 4 | |a cancer cells | |
| 650 | 4 | |a cell lines | |
| 650 | 4 | |a coculture | |
| 650 | 4 | |a culture models | |
| 650 | 4 | |a drug discovery | |
| 650 | 4 | |a heterogeneity | |
| 650 | 4 | |a in vitro | |
| 650 | 4 | |a microenvironment | |
| 650 | 4 | |a spheroid | |
| 650 | 4 | |a HNSCC | |
| 700 | 1 | |a Kern, Johann |d 1974- |e VerfasserIn |0 (DE-588)122749632X |0 (DE-627)1748494171 |4 aut | |
| 700 | 1 | |a Affolter, Annette |d 1977- |e VerfasserIn |0 (DE-588)124918417 |0 (DE-627)368073726 |0 (DE-576)294568190 |4 aut | |
| 700 | 1 | |a Rotter, Nicole |d 1970- |e VerfasserIn |0 (DE-588)118085638 |0 (DE-627)079235514 |0 (DE-576)178653888 |4 aut | |
| 700 | 1 | |a Lammert, Anne |d 1983- |e VerfasserIn |0 (DE-588)137533365 |0 (DE-627)695845284 |0 (DE-576)303406097 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Alternatives to laboratory animals |d London : SAGE Publications Ltd, 1981 |g 50(2022), 6 vom: Nov., Seite 414-422 |h Online-Ressource |w (DE-627)54650437X |w (DE-600)2390905-5 |w (DE-576)476810485 |x 2632-3559 |7 nnas |a Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives |
| 773 | 1 | 8 | |g volume:50 |g year:2022 |g number:6 |g month:11 |g pages:414-422 |g extent:9 |a Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives |
| 856 | 4 | 0 | |u https://doi.org/10.1177/02611929221135042 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230313 | ||
| 993 | |a Article | ||
| 994 | |a 2022 | ||
| 998 | |g 137533365 |a Lammert, Anne |m 137533365:Lammert, Anne |d 60000 |d 62100 |e 60000PL137533365 |e 62100PL137533365 |k 0/60000/ |k 1/60000/62100/ |p 5 |y j | ||
| 998 | |g 118085638 |a Rotter, Nicole |m 118085638:Rotter, Nicole |d 60000 |d 62100 |e 60000PR118085638 |e 62100PR118085638 |k 0/60000/ |k 1/60000/62100/ |p 4 | ||
| 998 | |g 124918417 |a Affolter, Annette |m 124918417:Affolter, Annette |d 60000 |d 62100 |e 60000PA124918417 |e 62100PA124918417 |k 0/60000/ |k 1/60000/62100/ |p 3 | ||
| 998 | |g 122749632X |a Kern, Johann |m 122749632X:Kern, Johann |d 60000 |d 62100 |e 60000PK122749632X |e 62100PK122749632X |k 0/60000/ |k 1/60000/62100/ |p 2 | ||
| 998 | |g 1227495234 |a Tenschert, Esther |m 1227495234:Tenschert, Esther |d 60000 |d 62100 |e 60000PT1227495234 |e 62100PT1227495234 |k 0/60000/ |k 1/60000/62100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1839065265 |e 4289058574 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"origin":[{"publisher":"SAGE Publications Ltd ; FRAME, Fund for the Replacement of Animals in Medical Experiments","dateIssuedDisp":"1981-","dateIssuedKey":"1981","publisherPlace":"London ; Nottingham"}],"id":{"eki":["54650437X"],"zdb":["2390905-5"],"issn":["2632-3559"]},"note":["Gesehen am 19.08.2021"],"name":{"displayForm":["published in association with FRAME, Fund for the Replacement of Animals in Medical Experiments"]},"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"title":[{"title_sort":"Alternatives to laboratory animals","title":"Alternatives to laboratory animals","subtitle":"ATLA"}],"recId":"54650437X","titleAlt":[{"title":"ATLA"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"pubHistory":["9.1981 -"],"corporate":[{"display":"Fund for the Replacement of Animals in Medical Experiments","role":"isb"}],"part":{"volume":"50","pages":"414-422","year":"2022","extent":"9","issue":"6","text":"50(2022), 6 vom: Nov., Seite 414-422"},"disp":"Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternativesAlternatives to laboratory animals"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"given":"Esther","role":"aut","display":"Tenschert, Esther","family":"Tenschert"},{"given":"Johann","role":"aut","family":"Kern","display":"Kern, Johann"},{"given":"Annette","role":"aut","display":"Affolter, Annette","family":"Affolter"},{"display":"Rotter, Nicole","family":"Rotter","role":"aut","given":"Nicole"},{"given":"Anne","family":"Lammert","display":"Lammert, Anne","role":"aut"}],"id":{"eki":["1839065265"],"doi":["10.1177/02611929221135042"]},"recId":"1839065265","origin":[{"dateIssuedKey":"2022","dateIssuedDisp":"November 2022"}],"name":{"displayForm":["Esther Tenschert, Johann Kern, Annette Affolter, Nicole Rotter, and Anne Lammert"]},"note":["Gesehen am 13.03.2023"],"physDesc":[{"extent":"9 S."}],"language":["eng"],"title":[{"title_sort":"Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives","title":"Optimisation of conditions for the formation of spheroids of head and neck squamous cell carcinoma cell lines for use as animal alternatives"}]} | ||
| SRT | |a TENSCHERTEOPTIMISATI2022 | ||